EFFECT OF NOISE STRESS-INDUCED NEUROBEHAVIORAL CHANGES ON WISTAR ALBINO RATS

Objective: The study aims to investigate the effect of acute noise stress on cognitive functions in male Wistar albino rats. Methods: Adult albino rats were randomly divided into two groups. Each group contains six animals. Rats exposed to acute noise stress (100 dB/4 h) were compared with control animal and assessed for cognition using T-maze, hole-board test, open-field test, marble burying test, and social interaction behavior. Results: The rats exposed to acute noise stress showed the significance (p<0.05) of behavioral alterations such as impaired learning and memory, memory retention, increased fear and anxiety, obsessive–compulsive behavior, social avoidance, and decreased social interaction. Conclusion: The results report that acute noise stress affects the cognition, and it became chronic may confer the increased risk of neurodegenerative disorders

EXPLORE THE IMPACT OF NOISE STRESS INDUCED BRAIN WAVE PATTERN AND BEHAVIOUR ALTERATIONS IN WISTAR ALBINO RATS

Objective: The aim of the study is to investigate the impact of noise-induced stress and electroencephalogram (EEG) with behavioral alteration in male Wistar albino Rats. Methods: Adult albino rats were randomly divided into three groups. Each group contains six animals. Rats exposed to acute and sub-acute noise, stress (100 dB/4 h) were compared with control animals and assessed for learning and memory using an Eight-arm radial maze, Y-maze, T-maze and also monitoring of brain electrical activity showed by the electro encephalography. Results: The reference memory and working memory error increases, in acute and sub-acute noise stress. The amplitude and frequency also increase in frontal and occipital lobar when compared to control animals. Conclusion: Animals were exposed to noise stress showed learning and memory impairment and also changes in EEG wave pattern

IMPACT OF CHRONIC NOISE ON HIPPOCAMPAL MORPHOLOGY AND ITS FUNCTIONS IN WISTAR ALBINO RATS

Objective: The study aims to investigate the effect of chronic noise stress on hippocampal morphology and its functions in male Wistar albino rats. Methods: Adult albino rats were randomly divided into two groups. Each group contained six animals. Rats exposed to chronic noise stress (100 dB/4 h–30 days) were compared with control animal and assessed for behavior using hole-board test, marble burying test, and morphology of hippocampus by histology. Results: The rats exposed to chronic noise stress showed significance (P < 0.05) of behavioral alterations such as increased fear and anxiety, obsessive-compulsive behavior, enlarged lateral ventricle, and reduced hippocampal volume. Conclusion: The results reported that chronic noise stress affects neurobehavioral due to reduced hippocampal volume

ROLE OF MICHELIA CHAMPACA IN MEMORY ENHANCEMENT AND ACUTE NOISE STRESSED MALE WISTAR ALBINO RATS

Objective: To identify the memory enhancing role of Michelia champaca in acute noise stressed animals. Methods: Male Wistar albino rats were used in this study. Animals were exposed to noise for 4 h before testing for memory. Thereafter, the plasma corticosterone level and acetylcholinesterase activity were estimated in the discrete regions of the brain, and the memory related behavior were assessed by eight arm radial maze.Results: Our results showed that Michelia champaca enhances the memory activity and decreases the corticosterone concentrations in acute noise stress animals treated with M. champaca. Moreover, it also decreased brain acetylcholinesterase activity when compared with the acute stress group (p<0.05). Furthermore, behavioral tests indicate that working memory, is enhanced by acute stress and decreases the error levels in all the parameters studied in the behavior aspects when compared to control animals.Conclusion: These findings suggest that Michelia champaca enhances the memory in albino rats and might be useful therapeutically for cognitive related dysfunctions. This could be due to the presence of memory boosting compounds and its antistressor and anti-acetylcholinesterase activity, thereby reduces the levels of serum corticosterone and inhibition of cholinesterase enzyme significantly

A United Kingdom survey of surgical technique and handling practice of inguinal canal structures during hernia surgery

Background: Recent trials have assessed the impact of elective nerve division on patient outcome after inguinal herniorrhaphy. The aim of this study was to establish UK surgical practice of handling of structures in the inguinal canal during herniorrhaphy. Methods: A cross-sectional survey of all Fellows (n = 1113) of the Association of Surgeons of Great Britain and Ireland (ASGBI) was performed. The main outcomes were to determine method of inguinal hernia repair and routine practice for intra-operative handling of structures in the inguinal canal. Results: A total of 852 (77%) questionnaires were returned, of which 784 (92%) surgeons performed inguinal herniorrhaphy. Approximately two-thirds (63%) of responding surgeons performed less than 50 procedures per annum and 37% conducted more than 50 procedures annually. Mesh was the preferred method used by 90% of surgeons; 6% used non-mesh, and 4% used other (laparoscopic) methods. Routine practice in relation to the inguinal structures varied by volume of hernia surgery; surgeons who conducted more than 50 procedures annually were more likely to visualize and preserve inguinal nerve structures. However, inconsistency in the answers suggested confusion over anatomy. Conclusion: This is the first UK survey to investigate method of hernia repair and usual handling practice of inguinal canal structures. There was wide acceptance of the use of mesh in inguinal hernia repair, with the majority of UK surgeons favoring an open approach. Surgeons performing high volumes of herniorrhaphy were more likely to preserve, rather than transect, inguinal nerve structures. This variation in practice may confound assessment of long-term neuralgia and other post-herniorrhaphy pain syndromes

EFFECT OF SLEEP DEPRIVATION ON REMINISCENCE AND APPREHENSION BEHAVIOR IN WISTAR ALBINO RATS

Objective: The aim of the present study is to investigate the effect of paradoxical sleep deprivation (SD) on learning and memory impairment and anxiety-like behavior in female Wistar albino rats. Methods: Eight-arm radial maze, open-field test, and light and dark test were used to assess the animals learning and memory and anxiety-like behavior. Results: SD associated with weaker learning and memory and increased anxiety- and depressive-like behavior in animals. Conclusion: Animals were exposed to SD showed learning and memory impairment and also exhibited increased anxiety- and depressive-like behavior when compared to control animals

LARVICIDAL POTENTIAL OF INDIGOFERA TINCTORIA (FABACEAE) ON DENGUE VECTOR (AEDES AEGYPTI) AND ITS ANTIMICROBIAL ACTIVITY AGAINST CLINICAL ISOLATES

 antimicrobial efficacy against clinical isolates.Methods: The extract was tested at various concentrations 64, 128, 256, and 512 mg/ml for antimicrobial activity and 0.1 and 5 mg/L were preparedfor larvicidal activity. The numbers of dead larvae were counted after 24 hrs of exposure.Result: The lowest minimum inhibitory concentration (MIC) values of the extract were 128 mg/ml against Klebsiella spp. - 1 alone and rest of theclinical test pathogens execute MIC activity at 512 mg/ml. The extract also showed antifungal activity with MIC of 64 mg/ml against the Candidaalbicans. Larvicidal activity of I. tinctoria extract were tested against fourth instar larvae A. aegypti and larval mortality were found after 24 hrs withlethal concentration (LC50)=3.1870 and LC90=5.3991 were observed.Conclusions: These results indicated that the extract displayed larvicidal potential on A. aegypti and antimicrobial activity against clinical isolates.Keywords: Infectious disease, Indigofera tinctoria, Antimicrobial activity, Larvicidal activity

A RANDOMIZED CASE–CONTROL PILOT STUDY ON THE NEUROCHEMICAL BASIS OF PAIN MODULATION IN PATIENTS WITH MIGRAINE, WHO PRACTICED INTEGRATED AMRITA MEDITATION TECHNIQUE

Objective: The objective of the study was to determine the changes in duration and intensity of headache and associated changes in the plasma levels of neurochemicals, serotonin, glutamate, vasoactive intestinal peptide (VIP), and nitric oxide (NO) in patients with migraine after 6 months of regular practice of integrated Amrita meditation (IAM). Methods: Sixteen patients aged 18–50 with migraine were randomly assigned to 2 groups, one with standard medical care and IAM, and the other only standard medical care. Data were collected before IAM, after 3 and 6 months of IAM practice Results: After 6 months, a significant decrease in the duration of headache from 2.4±0.54 to 1.4±0.54 (p=0.034) hours and intensity of pain from 3.6±0.54 to 2.6±0.89, (p=0.035) was seen in patients who practiced IAM. Plasma levels of serotonin within the IAM group increased (47.29±26.85 to 53.85±29.73ng/ml), where as there was decrease in glutamate (38.47±8.2 to 29.68±12.57μg/ml), VIP (28.01±13.64 to 22.23±7.79pg/ml) and NO levels (642.26±167.42 to 423.18±97.96μmol/L). A correlating trend was seen in comparison with control group after 6 months showing a statistically significant difference in plasma Serotonin (p value 0.007) and NO (p value-0.023) levels. Discussion: The results of our study have been discussed with other migraine and meditation-related studies. Conclusion: Regular practice of IAM reduces the intensity and duration of headache in migraine patients and is associated with alterations in the neurotransmitter levels

Parenting Styles and Academic Achievement among Malaysian Students: Mediating Role of Parental Involvement

The goal of the study is to see how parental participation affects the relationship between parenting styles and academic success in Malaysian primary school students. A quantitative technique was applied in this investigation. Private tuition centres in an urban area were used to recruit students. The information gathered for this investigation was numerically assessed and interpreted. Families are divided into four groups by the Parenting Style Index: authoritative, authoritarian, permissive, and indulgent. The relationship between parenting styles and academic success was studied using Pearson's correlation coefficient matrix. It was discovered that it completely mediates the relationship between authoritative and authoritarian parenting styles and student academic attainment. The study's findings show that understanding and receiving knowledge about how parenting styles and parental involvement affect children's academic achievement is crucial for parents

Big-pharmaceuticalisation:Clinical trials and contract research organisations in India

The World Trade Organisation's Trade Related Intellectual Property Rights [TRIPS] agreement aimed to harmonise intellectual property rights and patent protection globally. In India, the signing of this agreement resulted in a sharp increase in clinical trials since 2005. The Indian government, along with larger Indian pharmaceutical companies, believed that they could change existing commercial research cultures through the promotion of basic research as well as attracting international clinical trials, and thus create an international level, innovation-based drug industry. The effects of the growth of these outsourced and off-shored clinical trials on local commercial knowledge production in India are still unclear. What has been the impact of the increasing scale and commercialisation of clinical research on corporate science in India? In this paper we describe Big-pharmaceuticalisation in India, whereby the local pharmaceutical industry is moving from generic manufacturing to innovative research. Using conceptual frameworks of pharmaceuticalisation and innovation, this paper analyses data from research conducted in 2010-2012 and describes how Contract Research Organisations (CROs) enable outsourcing of randomised control trials to India. Focussing on twenty-five semi-structured interviews CRO staff, we chart the changes in Indian pharmaceutical industry, and implications for local research cultures. We use Big-pharmaceuticalisation to extend the notion of pharmaceuticalisation to describe the spread of pharmaceutical research globally and illustrate how TRIPS has encouraged a concentration of capital in India, with large companies gaining increasing market share and using their market power to rewrite regulations and introduce new regulatory practices in their own interest. Contract Research Organisations, with relevant, new, epistemic skills and capacities, are both manifestations of the changes in commercial research cultures, as well as the vehicles to achieve them. These changes have reinvigorated public concerns that stress not only access to new medicines but also the 'price' of innovation on research participants