Leveraging technology-driven strategies to untangle omics big data: circumventing roadblocks in clinical facets of oral cancer

Oral cancer is one of the 19most rapidly progressing cancers associated with significant mortality, owing to its extreme degree of invasiveness and aggressive inclination. The early occurrences of this cancer can be clinically deceiving leading to a poor overall survival rate. The primary concerns from a clinical perspective include delayed diagnosis, rapid disease progression, resistance to various chemotherapeutic regimens, and aggressive metastasis, which collectively pose a substantial threat to prognosis. Conventional clinical practices observed since antiquity no longer offer the best possible options to circumvent these roadblocks. The world of current cancer research has been revolutionized with the advent of state-of-the-art technology-driven strategies that offer a ray of hope in confronting said challenges by highlighting the crucial underlying molecular mechanisms and drivers. In recent years, bioinformatics and Machine Learning (ML) techniques have enhanced the possibility of early detection, evaluation of prognosis, and individualization of therapy. This review elaborates on the application of the aforesaid techniques in unraveling potential hints from omics big data to address the complexities existing in various clinical facets of oral cancer. The first section demonstrates the utilization of omics data and ML to disentangle the impediments related to diagnosis. This includes the application of technology-based strategies to optimize early detection, classification, and staging via uncovering biomarkers and molecular signatures. Furthermore, breakthrough concepts such as salivaomics-driven non-invasive biomarker discovery and omics-complemented surgical interventions are articulated in detail. In the following part, the identification of novel disease-specific targets alongside potential therapeutic agents to confront oral cancer via omics-based methodologies is presented. Additionally, a special emphasis is placed on drug resistance, precision medicine, and drug repurposing. In the final section, we discuss the research approaches oriented toward unveiling the prognostic biomarkers and constructing prediction models to capture the metastatic potential of the tumors. Overall, we intend to provide a bird’s eye view of the various omics, bioinformatics, and ML approaches currently being used in oral cancer research through relevant case studies

Impact of Coconut Husk Microcrystalline Cellulose on the Properties of Geopolymer Lightweight Concrete

Geopolymer composite is an alternative to ordinary Portland cement. It has potential to avoid CO2 emissions to the atmosphere and to save raw materials during its manufacture. Flyash-based geopolymer concrete is altered by adding ground granulated bast-furnace slag (GGBS) to improve its fresh and hardened properties. Thermal ash aggregate is used as coarse aggregate to reduce geopolymer concrete density, improve strength, and conserve natural aggregate. Along with this matrix, coconut husk microcrystalline cellulose (MCC) is added to enhance its performance. In a M40 grade flyash and GGBS-based geopolymer concrete, MCC was used to replace fly ash at 1% to 5% levels. The geopolymer composites were tested for slump, compression, split tensile, water absorption, and acid resistance to determine the way coconut husk MCC interacts with lightweight concrete. An inclusion of 3% MCC with geopolymer composites improved 2% slump, 6% of compressive and split tensile strength. About 1.6% of water absorption was reduced in GPC matrix with 3% of MCC. Meanwhile 3% of MCC in geopolymer concrete improved, 4% of weight and 7% of strength under acid exposure. The research strongly supported utilizing MCC in geopolymer concrete to render it more sustainable and eco-friendlier

Impact of Coconut Husk Microcrystalline Cellulose on the Properties of Geopolymer Lightweight Concrete

Geopolymer composite is an alternative to ordinary Portland cement. It has potential to avoid CO2 emissions to the atmosphere and to save raw materials during its manufacture. Flyash-based geopolymer concrete is altered by adding ground granulated bast-furnace slag (GGBS) to improve its fresh and hardened properties. Thermal ash aggregate is used as coarse aggregate to reduce geopolymer concrete density, improve strength, and conserve natural aggregate. Along with this matrix, coconut husk microcrystalline cellulose (MCC) is added to enhance its performance. In a M40 grade flyash and GGBS-based geopolymer concrete, MCC was used to replace fly ash at 1% to 5% levels. The geopolymer composites were tested for slump, compression, split tensile, water absorption, and acid resistance to determine the way coconut husk MCC interacts with lightweight concrete. An inclusion of 3% MCC with geopolymer composites improved 2% slump, 6% of compressive and split tensile strength. About 1.6% of water absorption was reduced in GPC matrix with 3% of MCC. Meanwhile 3% of MCC in geopolymer concrete improved, 4% of weight and 7% of strength under acid exposure. The research strongly supported utilizing MCC in geopolymer concrete to render it more sustainable and eco-friendlier

E-Governance Services Through Telecenters: The Role of Human Intermediary and Issues of Trust

Telecenter studies have shown that the centers become socially relevant only when they provide services in accordance with the needs of the local community. Earlier studies have highlighted the importance of a local intermediary in making telecenters successful. This article shows how trust between citizens and intermediaries at various levels affects the way e-governance services are delivered through telecenters. Drawing on the theoretical framework of the sociology of governance and taking an institutionalist perspective, this article highlights how the institutional membership of the intermediary is critical for effective e-governance service delivery. The article is based on an empirical study of the Akshaya telecenter project in Kerala. (c) 2007 by The Massachusetts Institute of Technology.

Full dynamic brain simulation using GATE in a high-performance computer

Dynamic PET brain studies are common in research and are becoming common in clinical applications. Simulation of dynamic PET is an important step to validate techniques and methods. This study compares a GATE simulation running on a CPU versus running on a GPU. We simulated the 3T Siemens MR-Brain PET with dynamic brain activity. The results show close agreement in the number of coincidence events, including phantom scatter. The code will be made available to the GATE community and the simulation data is available to the interested researchers

Hematopoietic Signaling Mechanism Revealed from a Stem/Progenitor Cell Cistrome

Thousands of cis-elements in genomes are predicted to have vital functions. Although conservation, activity in surrogate assays, polymorphisms, and disease mutations provide functional clues, deletion from endogenous loci constitutes the gold-standard test. A GATA-2-binding, Gata2 intronic cis-element (+9.5) required for hematopoietic stem cell genesis in mice is mutated in a human immunodeficiency syndrome. Because +9.5 is the only cis-element known to mediate stem cell genesis, we devised a strategy to identify functionally comparable enhancers (‘‘+9.5-like’’) genome-wide. Gene editing revealed +9.5-like activity to mediate GATA-2 occupancy, chromatin opening, and transcriptional activation. A +9.5-like element resided in Samd14, which encodes a protein of unknown function. Samd14 increased hematopoietic progenitor levels/activity and promoted signaling by a pathway vital for hematopoietic stem/progenitor cell regulation (stem cell factor/c-Kit), and c-Kit rescued Samd14 lossof- function phenotypes. Thus, the hematopoietic stem/progenitor cell cistrome revealed a mediator of a signaling pathway that has broad importance for stem/progenitor cell biology. (c) 2015 Elsevier Inc.161611sciescopu

Hematopoietic signaling mechanism revealed from a stem/progenitor cell cistrome

Thousands of cis-elements in genomes are predicted to have vital functions. Although conservation, activity in surrogate assays, polymorphisms, and disease mutations provide functional clues, deletion from endogenous loci constitutes the gold-standard test. A GATA-2-binding, Gata2 intronic cis-element (+9.5) required for hematopoietic stem cell genesis in mice is mutated in a human immunodeficiency syndrome. Because +9.5 is the only cis-element known to mediate stem cell genesis, we devised a strategy to identify functionally comparable enhancers ("+9.5-like'') genome-wide. Gene editing revealed +9.5-like activity to mediate GATA-2 occupancy, chromatin opening, and transcriptional activation. A +9.5-like element resided in Samd14, which encodes a protein of unknown function. Samd14 increased hematopoietic progenitor levels/activity and promoted signaling by a pathway vital for hematopoietic stem/progenitor cell regulation (stem cell factor/c-Kit), and c-Kit rescued Samd14 loss-of-function phenotypes. Thus, the hematopoietic stem/progenitor cell cistrome revealed a mediator of a signaling pathway that has broad importance for stem/progenitor cell biology.