Spain and European Union Constitution-building

The broad objective of this paper is to better understand how national governments form their policy position on the Draft Constitutional proposal by analysing developments in Spain. It examines the Spanish position on various parts of the European Convention based on data from expert interviews carried out in November 2003. It then evaluates the internal coordination process, focusing on the few domestic-level actors (from the Prime Ministers’ Office and the Ministries of Foreign Affairs and Economy) that have been most influential in shaping Spain’s position. Finally, it ponders the potential shift in the Spanish position given the recent Socialist victory in March 2004

Stabilization of HPV16 E6 protein by PDZ proteins, and potential implications for genome maintenance

AbstractThe E6 protein from high-risk human papillomaviruses appears necessary for persistence of viral episomes in cells but the underlying mechanism is unclear. E6 has many activities, including its ability to bind and degrade PDZ domain-containing proteins, such as hScrib. However little is known about the role of these interactions for E6 function and the viral life cycle. We now show that the levels of expression of wild-type E6 are increased in the presence of hScrib whilst a mutant E6 protein lacking the PDZ-binding motif is found at lower levels as it is turned over more rapidly by the proteasome. This correlates with an inability of genomes containing this mutation to be maintained as episomes. These results show that E6 association with certain PDZ domain-containing proteins can stabilize the levels of E6 expression and provides one explanation as to how the PDZ-binding capacity of E6 might contribute to genome episomal maintenance

Cell-free Embryonic Stem Cell Extract-mediated Derivation of Multi-potent Stem Cells from NIH3T3 Fibroblasts for Functional and Anatomical Ischemic Tissue Repair

The oocyte-independent generation of multipotent stem cells is one of the goals in regenerative medicine. We report that upon exposure to mouse ES cell (ESC) extracts, reversibly permeabilized NIH3T3 cells undergo de-differentiation followed by stimulus-induced re-differentiation into multiple lineage cell types. Genome-wide expression profiling revealed significant differences between NIH3T3 and ESC-extract treated NIH3T3 cells including re-activation of ESC specific transcripts. Epigenetically, ESC extracts induced CpG de-methylation of Oct4 promoter, hyper-acetylation of histones 3 and 4 and decreased lysine 9 (K-9) dimethylation of histone 3. In mouse models of surgically-induced hind limb ischemia (HLI) or acute myocardial infarction (AMI) transplantation of reprogrammed NIH3T3 cells significantly improved post-injury physiological functions and showed antomical evidence of engraftment and trans-differentiation into skeletal muscle, endothelial cell and cardiomyocytes. These data provide evidence for the generation of functional multi-potent stem like cells from terminally differentiated somatic cells without the introduction of trans-genes or ESC fusion

Performance of a Pilot-Scale Air Sparged Continuous Flow Reactor and Hydrocyclone for Struvite Precipitation and Removal from Liquid Swine Manure

The objective of this research was to test a pilot-scale air sparged tank reactor (ASTR) and the ASTR in combination with a hydrocyclone (called the pilot-scale ASTR-hydrocyclone system) on two swine manure slurries for struvite-based (MgNH4PO4-6H2O) phosphorus removal and recovery. The pilot-scale ASTR system operated at flow rates of 80 to 115 L/min and was based on the bench-scale design from Shepherd et al. (2007). The ASTR effluent was processed using a hydrocyclone separator for struvite separation and total phosphorus (TP) recovery. The pilot-scale ASTR-hydrocyclone system provided a 92% reduction of dissolved reactive phosphorus (DRP) in manure slurry from a swine finishing facility concrete storage tank and a 91% reduction of DRP in manure slurry collected from a swine finishing facility deep-pit under floor collection system. The pilot-scale ASTR-hydrocyclone system removed 18% of TP in swine manure from a concrete storage tank and 9% to 14% of TP in swine manure slurry from a deep-pit under floor collection system. The low TP recovery was attributed to the hydrocyclones inability to provide effective struvite separation as operated. Full-scale economics and implementation of the tested struvite-based phosphorus removal is discussed. A case study of a typical Iowa deep-pit swine production facility (10,000 head/year) indicated that the annual cost of struvite-based phosphorus removal using this system would be approximately $8.88/finished pig or$0.035/L manure slurry treated ($ 0.134/gal). This cost often exceeds producer\u27s profit margins; this indicates that struvite-based phosphorus removal using this ASTR-hydrocyclone system in swine finisher manure slurries is not currently economically viable

Simulations and Measurements of the Background Encountered by a High-Altitude Balloon-Borne Experiment for Hard X-ray Astronomy

We have modelled the hard X-ray background expected for a high-altitude balloon flight of the Energetic X-ray Telescope Experiment (EXITE2), an imaging phoswich detector/telescope for the 20--600 keV energy range. Photon and neutron-induced contributions to the background are considered. We describe the code and the results of a series of simulations with different shielding configurations. The simulated hard X-ray background for the actual flight configuration agrees reasonably well (within a factor of $\sim$ 2) with the results measured on the first flight of EXITE2 from Palestine, Texas. The measured background flux at 100 keV is $\sim$ 4 $\times$ 10$^{-4}$ counts cm$^{-2}$ s$^{-1}$ keV$^{-1}$.Comment: 17 pages Latex (uses aaspp4.sty) plus 7 postscript figures: available in file figs.tar.g