The Combined Impact Of IgLON Family Proteins Lsamp And Neurotrimin On Developing Neurons And Behavioral Profiles In Mouse

Cell surface neural adhesion proteins are critical components in the complex orchestration of cell proliferation, apoptosis, and neuritogenesis essential for proper brain construction and behavior. We focused on the impact of two plasticity-associated IgLON family neural adhesion molecules, Neurotrimin (Ntm) and Limbic system associated membrane protein (Lsamp), on mouse behavior and its underlying neural development. Phenotyping neurons derived from the hippocampi of Lsamp−/−, Ntm−/− and Lsamp−/−Ntm−/− mice was performed in parallel with behavioral testing. While the anatomy of mutant brains revealed no gross changes, the Ntm−/− hippocampal neurons exhibited premature sprouting of neurites and manifested accelerated neurite elongation and branching. We propose that Ntm exerts an inhibitory impact on neurite outgrowth, whereas Lsamp appears to be an enhancer of the said process as premature neuritogenesis in Ntm−/− neurons is apparent only in the presence of Lsamp. We also show interplay between Lsamp and Ntm in regulating tissue homeostasis: the impact of Ntm on cellular proliferation was dependent on Lsamp, and Lsamp appeared to be a positive regulator of apoptosis in the presence of Ntm. Behavioral phenotyping indicated test-specific interactions between Lsamp and Ntm. The phenotypes of single mutant lines, such as reduced swimming speed in Morris water maze and increased activity in the elevated plus maze, were magnified in Lsamp−/−Ntm−/− mice. Altogether, evidence both from behavioral experiments and cultured hippocampal cells show combined and differential interactions between Ntm and Lsamp in the formation of hippocampal circuits and behavioral profiles. We demonstrate that mutual interactions between IgLON molecules regulate the initiation of neurite sprouting at very early ages, and even cell-autonomously, independent of their regulation of cell-cell adhesion

In Vitro Study of MefenamateStarch as Drug Delivery System

Mefenamic acid was esterified with starchwith[1:1] Molar ratio, as drug substituted with natural polymer, to prolongthe period of hydrolysis of drug polymer with other advantages. The new prodrug starch was characterized by FT-IR and UV-Visible and 1H-NMR spectroscopies. The physical properties were studied and controlled drug release was studied in different pH values at 37oC. The stability of drug was carried out by measuring the absorbance of mefenamic starch which hydrolyzed in HCl solution of pH 1.1 (artificial gastric fluid) and phosphate buffer of pH 7.4 (simulating intestinal fluid SIF) at 37oC for several days. The thermal analysis such as DSC was studied

Classical Casimir interaction in the plane-sphere geometry

We study the Casimir interaction in the plane-sphere geometry in the classical limit of high temperatures. In this limit, the finite conductivity of the metallic plates needs to be taken into account. For the Drude model, the classical Casimir interaction is nevertheless found to be independent of the conductivity so that it can be described by a single universal function depending only on the aspect ratio $x=L/R$ where $L$ is the interplate distance and $R$ the sphere radius. This universal function differs from the one found for perfect reflectors and is in principle amenable to experimental tests. The asymptotic approach of the exact result to the Proximity Force Approximation appears to be well fitted by polynomial expansions in $\ln x$.Comment: Updated version with minor modifications and addition of a referenc

Fluctuation induced quantum interactions between compact objects and a plane mirror

The interaction of compact objects with an infinitely extended mirror plane due to quantum fluctuations of a scalar or electromagnetic field that scatters off the objects is studied. The mirror plane is assumed to obey either Dirichlet or Neumann boundary conditions or to be perfectly reflecting. Using the method of images, we generalize a recently developed approach for compact objects in unbounded space [1,2] to show that the Casimir interaction between the objects and the mirror plane can be accurately obtained over a wide range of separations in terms of charge and current fluctuations of the objects and their images. Our general result for the interaction depends only on the scattering matrices of the compact objects. It applies to scalar fields with arbitrary boundary conditions and to the electromagnetic field coupled to dielectric objects. For the experimentally important electromagnetic Casimir interaction between a perfectly conducting sphere and a plane mirror we present the first results that apply at all separations. We obtain both an asymptotic large distance expansion and the two lowest order correction terms to the proximity force approximation. The asymptotic Casimir-Polder potential for an atom and a mirror is generalized to describe the interaction between a dielectric sphere and a mirror, involving higher order multipole polarizabilities that are important at sub-asymptotic distances.Comment: 19 pages, 7 figure

Geometry and material effects in Casimir physics - Scattering theory

We give a comprehensive presentation of methods for calculating the Casimir force to arbitrary accuracy, for any number of objects, arbitrary shapes, susceptibility functions, and separations. The technique is applicable to objects immersed in media other than vacuum, to nonzero temperatures, and to spatial arrangements in which one object is enclosed in another. Our method combines each object's classical electromagnetic scattering amplitude with universal translation matrices, which convert between the bases used to calculate scattering for each object, but are otherwise independent of the details of the individual objects. This approach, which combines methods of statistical physics and scattering theory, is well suited to analyze many diverse phenomena. We illustrate its power and versatility by a number of examples, which show how the interplay of geometry and material properties helps to understand and control Casimir forces. We also examine whether electrodynamic Casimir forces can lead to stable levitation. Neglecting permeabilities, we prove that any equilibrium position of objects subject to such forces is unstable if the permittivities of all objects are higher or lower than that of the enveloping medium; the former being the generic case for ordinary materials in vacuum.Comment: 44 pages, 11 figures, to appear in upcoming Lecture Notes in Physics volume in Casimir physic

Study of Optimal Perimetric Testing In Children (OPTIC): Development and feasibility of the kinetic perimetry reliability measure (KPRM)

INTRODUCTION: Interpretation of perimetric findings, particularly in children, relies on accurate assessment of test reliability, yet no objective measures of reliability exist for kinetic perimetry. We developed the kinetic perimetry reliability measure (KPRM), a quantitative measure of perimetric test reproducibility/reliability and report here its feasibility and association with subjective assessment of reliability. METHODS: Children aged 5-15 years, without an ophthalmic condition that affects the visual field, were recruited from Moorfields Eye Hospital and underwent Goldmann perimetry as part of a wider research programme on perimetry in children. Subjects were tested with two isopters and the blind spot was plotted, followed by a KPRM. Test reliability was also scored qualitatively using our examiner-based assessment of reliability (EBAR) scoring system, which standardises the conventional clinical approach to assessing test quality. The relationship between KPRM and EBAR was examined to explore the use of KPRM in assessing reliability of kinetic fields. RESULTS: A total of 103 children (median age 8.9 years; IQR: 7.1 to 11.8 years) underwent Goldmann perimetry with KPRM and EBAR scoring. A KPRM was achieved by all children. KPRM values increased with reducing test quality (Kruskal-Wallis, p=0.005), indicating greater testretest variability, and reduced with age (linear regression, p=0.015). One of 103 children (0.97%) demonstrated discordance between EBAR and KPRM. CONCLUSION: KPRM and EBAR are distinct but complementary approaches. Though scores show excellent agreement, KPRM is able to quantify withintest variability, providing data not captured by subjective assessment. Thus, we suggest combining KPRM with EBAR to aid interpretation of kinetic perimetry test reliability in children

On the accuracy of the PFA: analogies between Casimir and electrostatic forces

We present an overview of the validity of the Proximity Force Approximation (PFA) in the calculation of Casimir forces between perfect conductors for different geometries, with particular emphasis for the configuration of a cylinder in front of a plane. In all cases we compare the exact numerical results with those of PFA, and with asymptotic expansions that include the next to leading order corrections. We also discuss the similarities and differences between the results for Casimir and electrostatic forces.Comment: 17 pages, 5 figures, Proceedings of the meeting "60 years of Casimir effect", Brasilia, 200

Molecular characterization of Leishmania species from stray dogs and human patients in Saudi Arabia

Leishmania major and Leishmania tropica cause cutaneous leishmaniasis in humans and dogs in several parts of the world, with a large number of cases recorded in the Middle East. However, when they occur in sympatry, the role of each species of Leishmania in the epidemiology of cutaneous leishmaniasis (CL) is not clear. To assess the frequency and to identify the species of Leishmania that infect humans and stray dogs in Riyadh and Al-Qaseem (Saudi Arabia), 311 stray dogs and 27 human patients who were suspected for Leishmania infection were examined for CL by a nested polymerase chain reaction (nPCR). Seven (25.9%) out of 27 human patients scored positive for Leishmania spp. (i.e., L. major in five patients from Riyadh and L. tropica in two patients from Al-Qaseem). Out of 311 dogs, five (1.6%) were infected by L. tropica. Data herein presented demonstrate the occurrence of L. tropica in dogs and humans in Saudi Arabia, as well as the occurrence of L. major in humans

Casimir forces between arbitrary compact objects: Scalar and electromagnetic field

We develop an exact method for computing the Casimir energy between arbitrary compact objects, both with boundary conditions for a scalar field and dielectrics or perfect conductors for the electromagnetic field. The energy is obtained as an interaction between multipoles, generated by quantum source or current fluctuations. The objects' shape and composition enter only through their scattering matrices. The result is exact when all multipoles are included, and converges rapidly. A low frequency expansion yields the energy as a series in the ratio of the objects' size to their separation. As examples, we obtain this series for two spheres with Robin boundary conditions for a scalar field and dielectric spheres for the electromagnetic field. The full interaction at all separations is obtained for spheres with Robin boundary conditions and for perfectly conducting spheres.Comment: 24 pages, 3 figures, contribution to QFEXT07 proceeding