Management of lower urinary tract obstructive disease using bladder tube cystotomy in a Saanen buck

This report describes a procedure of bladder tube cystotomy to relieve progressing bladder distension in a goat diagnosed to have obstructive urolithiasis. This one-year old Saanen buck was presented with a complaint of not passing urine and being in discomfort for almost three days. On physical examination, pulsation of the urethra in the perineal region could be felt and swelling and pain along the prepuce and perineum were indicated on deep palpation. Amputation of the urethral orifice and attempt to catheterise the urethra failed to relieve bladder distension; thus, bladder tube cystotomy procedure was performed. Following this procedure, all vital parameters returned to normal and normograde cystourethrogram was performed to radiographically assess the lower urinary tract of the goat. The outcome of this case supports that the bladder tube cystotomy technique provides an effective method to allow immediate relief of a distended bladder, allowing the urethra to heal as the urine outflow is diverted through the catheter and allows normograde cystourethrography to be performed

Urinary bladder rupture secondary to obstructive urolithiasisin a Jamnapari goat

This report describes the complications of obstructive urolithiasis in the lower urinary tract causing urinary bladder rupture in a Jamnapari buck. A 3-year-old Jamnapari buck was presented with the complaint of stranguria, subsequent anuria and a progressively distended abdomen for the past three days. Upon physical examination, body temperature, pulse rate and respiration rate were increased. Uroliths could be felt within the urethra in the ventral abdomen region. A urolith was removed via amputationof the urethral process, but the patency of the urethra could not be established. Transabdominal ultrasound revealed anechoic areas around the bladder, and the bladder was relatively small for a urinary obstructed goat. The bladder wall was thickened and shadow of sludge was observed within the bladder. Abdominocentesis was done and fluid analysis revealed that it was a haemorrhagic effusion. Blood results revealed renal disease, liver disease, muscle injury and haemoconcentration. Retrograde cystourethrogram revealed no urolithswithinthe urethra but there was leakage of the contrast agent from the bladder into the peritoneal cavity. The final diagnosis was complete blockage of the lower urinary tract leading to bladder rupture. Exploratory laparotomy was done and emergency cystorraphy was planned. Due to the poor condition of the urinary bladder with presence of septic peritonitis, the goat was euthanised

Seroprevalence and molecular detection of leptospirosis from a dog shelter

A study on seroprevalence and molecular detection of canine leptospirosis was carried out in a dog population (randomly selected n=80 dogs) from an animal shelter X. All the dogs in Shelter X appeared clinically healthy. Eighty blood samples were obtained and their serum were serologically examined using Microscopic Agglutination Test (MAT) against 10 Leptospira serovars. Plasma samples obtained were subjected to Polymerase Chain Reaction (PCR) assay. Three out of 80 dogs (3.8%) tested positive for L. bataviae based on MAT at a titer of 1:80. The seroprevalence of 9 other Leptospira serovars was not evident in this study. All the dogs tested negative against leptospirosis with PCR assay. In conclusion, canine leptospirosis was detected in dogs in this animal shelter. L. bataviae was identified as the infecting serovar. To our knowledge, this is the second report of serovar Bataviae infection in dogs in Malaysia. The 3 dogs in our study could possibly be a source of leptospiral infection to other dogs and may shed the bacteria into the environment. This serovar is not available in canine vaccination programs, therefore the dogs are not protected from this disease. Further investigation is warranted to determine whether the infected dogs are carriers of this serovar

Prevalence of leptospirosis in healthy dogs and dogs with kidney disease in Klang Valley, Malaysia

Leptospirosis is under-reported and remarkably neglected in Malaysia, especially in companion animals. In recent years, dogs have become popular pets and potentially act as one of the risk factors for human leptospiral infection. The purpose of this study was to determine the serological and molecular status of leptospirosis in healthy and dogs with kidney disease in Klang Valley, Malaysia and to gain insight of the possible serovars involved in the dog population in Klang Valley, Malaysia. Blood samples were obtained from 57 dogs (19 kidney disease patient; 38 healthy dogs, respectively). Serum samples obtained from these animals were screened for leptospiral antibodies by the microscopic agglutination test (MAT). Polymerase chain reaction (PCR) assay was performed on plasma samples to detect leptospiral DNA. By MAT, three out of 19 (15.8%) dogs with kidney disease were positive for L. canicola. One out of 38 (2.6%) healthy dogs was positive for L. icterohemorrhagiae. The overall seroprevalence for leptospirosis in dogs in Klang Valley, Malaysia was 7.0% (n=4/57). Only one out of the 19 dogs (5.3%) with kidney disease was tested positive to pathogenic Leptospira by PCR assay. All the 38 healthy dogs were negative. Positive results in healthy dogs and dogs with kidney disease for leptospirosis warrant further investigation of leptospirosis in dog population in Malaysia. The prevalence and incidence of this disease in the dog population in this country need further investigation

Evaluation of leptospirosis knowledge, attitude and practice among dog handlers

Dogs could be reservoirs and carriers of Leptospira spp., hence, may play a potential role in disease transmission, exposing dog handlers to leptospirosis. This study evaluated the dog handlers’ knowledge, attitude and practice (KAP) towards leptospirosis. Four working dog and four dog shelter organisations were approached. A total of 138 dog handlers were conveniently recruited and evaluated on their level of knowledge, attitude and practice towards leptospirosis using a validated structured questionnaire. Most of the dog handlers were male aged between 30 and 40 years with a wide range of working experience of 1 - 10 years and all of them have no formal training about leptospirosis. The majority of the dog handlers from the dog shelters had poor knowledge (90%, n=34/38) and attitude (100%, n=38/38) and moderate practice (53%, n=20/38) towards leptospirosis, whereas the working dog handlers had poor knowledge (58%, n=58/100) and attitude (75%, n=75/100) but a good level of practice (77%, n=77/100). The poor attitude towards leptospirosis in both groups is alarming as it may hinder implementation plans of control and preventive measures. Although there was good hand-washing practices among the dog handlers, there was a lack of personal protective equipment (PPE) practice that may therefore expose both the dogs and dog handlers to leptospirosis. Information gained from this study could be used to implement intervention programmes and to suggest prevention measures to limit the risk of leptospirosis among the dog handlers

Clinicopathologic and radiographic features in 40 cats diagnosed with pulmonary and cutaneous Rhodococcus equi infection (2012-2018)

Objectives: This retrospective study aimed to describe clinical manifestations, diagnostic options, radiological features, therapeutic plans and outcomes for cats infected with Rhodococcus equi. Methods: Forty cats aged between 2 months and 11 years old (median 6 months) that were definitively diagnosed with rhodococcosis between 2012 and 2018 were recruited in this study. Medical records were reviewed for information on signalment, history, clinical presentation, diagnostic testing, treatment plans and clinical outcomes. Results Of the 40 cats, 36 showed the pulmonary form of the disease, with 35 (87.5%) presenting with dyspnoea, while four cats presented with only cutaneous lesions. Mean body temperature was 38.7±0.2°C. Dyspnoea was noted in 87.5% of the cats. Leukocytosis (58.3%) with band neutrophilia (83.3%), monocytosis (58.3%) and thrombocytopenia (55.5%) were prominent findings in the haematology reports. Hyperproteinaemia (61.1%) with hypoalbuminaemia (22.2%) and hyperglobulinaemia (63.8%) with a low albumin:globulin ratio (38.9%) were prominent features of blood biochemistry reports. An alveolar–interstitial pattern was noted in 75% of prethoracocentesis radiographs. Pleural effusion, hepatomegaly, thoracic lymphadenopathy and atelectasis of any lung lobe were seen in 88.9%, 75%, 41.7% and 36.1% of cats, respectively. Overall, the mortality rate was 67.5% in both forms. Conclusions and relevance Clinicians should be aware that feline rhodococcosis manifests as a pulmonary disease at a much higher rate than previously reported. Further studies are required to address the epidemiology, pathophysiology, disease management and prognosis of feline rhodococcosis. The role of immunosuppression as a predisposing factor in feline rhodococcosis requires further investigation

Serological detection of anti-leptospira antibodies among animal caretakers, dogs and cats housed in animal shelters in Peninsular Malaysia

Leptospirosis is a worldwide zoonotic disease caused by Leptospira spp. and it has emerged as one of the major public health issues worldwide. Despite extensive studies, information regarding leptospirosis in animal shelters in Malaysia is still scarce. The aim of this study was to determine anti-Leptospira antibodies and the most common circulating serogroups among humans and animals in animal shelters in west and south parts of Peninsular Malaysia. Blood samples were obtained from 58 humans, 127 dogs, and 47 cats, that were recruited from two shelters. All humans and dogs appeared healthy, except few cats showed clinical signs of mild feline upper respiratory disease. Microscopic Agglutination Test (MAT) was used to detect anti-Leptospira antibodies against 20 pathogenic serovars. Based on the cut-off antibody titre ≥ 1:100, the sero-detection of Leptospira spp. in human, dogs, and cats were as 8.62%, 20.47%, and 14.89%, respectively. Serogroup Bataviae was found in human, dogs and cats, whereas Bataviae, Javanica, and Ballum were the common serogroups among dogs and cats. Anti-Leptospira antibodies titres were in the range from 1:100 to 1:200 in human and 1:100 to 1:400 in sheltered animals. Sero-detection studies of anti-Leptospira antibodies in shelters environment worldwide and regionally is necessary to increase the public health awareness and to understand the risk of this zoonotic disease. Furthermore, data regarding the predominant serogroups is needed in a local setting for further vaccination development studies. More studies are warranted to investigate the role of sheltered animals in leptospiral transmission in its environment

Clinicopathological and Radiological Features of Cats Presented with Infectious Respiratory Disease Signs: A Focus on <i>Rhodococcus equi</i> and <i>Klebsiella pneumoniae</i>

The objective of this study was to evaluate the prevalence of involvement of common viral organisms R. equi and K. pneumoniae and their clinicopathological and radiological features in respiratory disease of Malaysian domestic cats. A total of 34 feline cases with acute/chronic infectious respiratory disease signs were followed prospectively to investigate respiratory disease due to R. equi and K. pneumoniae and their relationship with concurrent viral infections in disease manifestation. All sampled cats (n = 27) were positive for FCoV antibodies and negative for FeLV. A significantly high antibody titer for FCV in n = 26 cases was also noticed. A single sample of pyothorax from a 3-months-old, non-vaccinated kitten was positive for R. equi. Bronchopneumonia with severe infiltration of the polymorphs and mononuclear inflammatory cells were prominent features of lungs histopathology from the kitten positive for R. equi. K. pneumoniae subsp. pneumoniae was confirmed from tracheal swabs of two cats. Histologically, the tracheal tissues of the two cats positive for K. pneumoniae were normal. In diagnostic imaging, epicenter of the infectious URT disease was nasal conchae rostrally and nasal turbinates caudally, however for infectious LRT disease was bronchial tree. Conclusively, infectious respiratory disease is a complex illness in cats, predominantly for unvaccinated kittens and young adult cats, especially those kept in multi-cat household or shelter environments because of the involvement of multiple bacterial and viral organisms as primary or secondary invaders. Clinicians should not preclude feline rhodococcosis from differentials, especially in kittens with pyothorax and less than one year of age. Unlike R. equi, K. pneumoniae has the potential to colonize URT of cats which might be disseminating further to cause LRT disease

Canine vertebral screw and rod fixation system in dogs

Bone plates and screws are often recommended to fix vertebral fracture and luxation in dogs although several complications had been reported. The canine vertebral screw and rod fixation (CVSRF) system, a device tailored for the canine spine, is a modified system from the human pedicle screw. This study aimed to determine the optimal corridor implantation of CVSRF and to investigate the potential trauma to the vertebrae and spinal cord in medium-sized dogs. Two screws of 16 mm and 20 mm and rods of 40 mm and 45 mm in length were inserted into the pedicles of L1 and L2 in six dogs. Safe implantation angles for 16 mm screw were 52.67° ± 10.40ºand 58.59° ± 7.72ºat L1 and L2, respectively. The angle of the 20 mm screw at L1 was recorded at 56.03°±5.34ºand 55.67° ± 2.89ºat L2. No gross and histological lesion was found on the spinal cord and vertebrae although minimal microfractures of the vertebrae were observed histologically. Findings from this study suggest that CVSRF is feasible for medium-sized dogs using 16 mm screws, however, a long-term study is required to determine the stability and durability of the system

Shedding and genetic diversity of Leptospira spp. from urban stray dogs in Klang Valley, Malaysia

Leptospirosis is an endemic zoonoses of global proportions. Stray dogs have been postulated to play a role in disease transmission; however, supporting information are still limited. Roaming behavior may not only predispose the dogs to infection, but could also contribute to disease spread. In this study, the susceptibility of urban stray dogs in shedding Leptospira spp. was determined. Blood, urine, and tissue samples of kidney and liver were collected from 100 dogs from 2 animal control facilities. Serological testing using microscopic agglutination test (MAT) were performed on blood against 20 leptospiral serovars with a cut-off titre of ≥ 1:100. Samples were cultured onto semi-solid Ellinghausen and McCullough modified by Johnson and Harris (EMJH) media. Isolates were identified using molecular polymerase chain reaction (PCR) using 2 primers (16s rRNA and LipL32) and hyperimmune serum (HIS) MAT. The seroprevalence for the dogs positive for leptospirosis was 32% (n=32/100) with the following detected serovars: Javanica (n=13), Bataviae (n=10), Icterohaemorrhagiae (n=3), Autumnalis (n=2), Canicola (n=1), Pyrogenes (n=1), Copenhageni (n=1), and Australis (n=1). Six Leptospira spp. isolated were procured from urine (n=2), kidney (n=2) and liver (n=2). All 6 isolates belonged to L. interrogans, a pathogenic variant of Leptospira spp. Serotyping and phylogenetic analysis suggested serovar Bataviae (n=5) and serovar Canicola (n=1). Presence of vaccinal serovars (Icterohaemorrhagiae and Canicola) suggested potential post-vaccination antibodies but the predominance of non-vaccinal serovars (Javanica and Bataviae) indicate the possibility of current infection or post-exposure. Isolation of Leptospira spp. directly from urine sample not only suggested an active infection but highlighted the potential shedding capability among these stray dogs. These findings further strengthen speculations that urban stray dogs could play a role in transmission and dissemination of leptospirosis through their constant movement. The urine of infected dogs may contaminate the environment, posing a major public health threat