Assessment of Grasslands and Livestock Production in Kangra Valley of Himachal Pradesh

In Himachal Pradesh 89.96 percent as per 2011 census of population lives in rural areas. The mainstay of the people of Himachal Pradesh is agriculture and has an important place in the economy of the State. Agricultural census shows that 87.95 percent of the total holdings are of small and marginal. Rearing of livestock is an integral component of rural economy (Anonymous, 2014). Livestock depend to a certain extent on fodder and grass grown on common property resources (CPR) as well as on crops and residues. Animal production is an integral part and forms part of the earning of small and marginal farmers. Almost every household in the State maintains a few heads of livestock of one kind or the other. The indigenous livestock population, and in many cases their cross bred progeny are dependent on grazing/pasture land and forest. When these animals become unproductive, old or sick, there is a tendency to abandon them rather than be responsible for feeding them. Grassland/pastures produce far below their potential and there is a gap between demand and supply of green fodder. The geographical area of Kangra is 5, 63,832.3 ha and area under grasslands is 69,781.7 ha that comes around 27. 51%. (Singh et al., 2009). The problem of animal productivity has been exacerbated by the shortage of fodder as holdings have become smaller and the extent and productivity of common grazing lands has also reduced over time. There are mainly two reasons which are responsible for poor performance of livestock, i.e., low productive animals and low availability of fodder

Integrated Nutrient Management for Natural Grasslands of Mid-Hills of Himalayas

Livestock rearing is an important pursuit in mountain farming in India and plays a crucial role throughout the country. The preponderance of marginal and small landholdings (about 82%) in hilly regions does not allow the farmers to allocate even a small part of their land exclusively for forage production. In Himachal Pradesh state of India about 1.16 m ha (20% of the total area) is under permanent pastures and other grazing lands and none of the natural grasslands are fertilised in any form. Existing grasslands have deteriorated to such an extent that their carrying capacity is only 1.05 ACU (Adult Cattle Unit, with an average body weight of 350 kg)/ha (Vashist et al., 2000). Biofertiliser-based technologies could be appropriate and cost effective approaches that are easy to adopt and eco-friendly. Response may arise from increased populations of phosphate solubilisers in the rhizosphere in P- deficient soils resulting in mobilisation of insoluble phosphorus (Raghu & Mac Rac, 1967). The study was undertaken with the main objectives of assessing the effects of biofertilisers on productivity and quality of natural grassland and the level of N and P substitution by biofertilisers

Identification and Characterization of Pastureland and Other Grazing Resources of Jammu & Kashmir Using GIS and Satellite Remote Sensing Technique

Livestock acquires special importance in mountain farming system from both ecological and socioeconomic point of view. They are an integral part of the farming system and a “bridge’’ connecting two types of land viz., forest and cultivated. In hilly regions, the arable agriculture is not very remunerative; therefore farmers of all categories rear sheep, goats, cattle etc. under sedentary, semi-migratory and migratory systems to supplement family income (Dev et al., 2014). The common property resources (CPR) and degraded pasturelands are the major feed sources. Remotely sensed satellite imagery and Geographic Information System (GIS) are being widely used for the assessment of pasturelands and estimation of forage availability as well as monitoring of the range resources (Singh et al., 2011). Since, the grazing resources are limited and natural grasslands are becoming scarcer, there is a greater need to effectively manage grasslands for optimum forage production and eco-development (Roy and Singh, 2013). Precise information on extent, condition and forage availability is essential for developmental planning. In the present study information on current status of pasturelands in Jammu and Kashmir based on remotely sensed satellite data and GIS/GPS is carried out

The role of immunohistochemistry in medullomyoblastoma – a case series highlighting divergent differentiation

<p>Abstract</p> <p>Aims</p> <p>To analyse the histo-morphology of cases of medullomyoblastoma and identifying its divergent differentiation.</p> <p>Methods</p> <p>A retrospective review of all cases reported as medulloblastoma between the period of Jan 2000 to Dec 2006 was carried out on Hematoxylin and eosin (H & E) stained slides. The cases were screened on light microscopy for primitive neuroectodermal component of a medulloblastoma accompanied by areas of "myoid" differentiation, identified on the basis of presence of strap cells (indicating a clear skeletal muscle differentiation) and/or large anaplastic cells with vescicular nuclei and moderate to abundant amount of eosinophilic cytoplasm. All these cases were subjected to a panel of immunohistochemical stains, including Desmin, GFAP, NFP, HMB45, SMA, S100, CK and EMA. Ultrastructral analysis was done on tissue obtained from paraffin blocks in 2 cases.</p> <p>Results</p> <p>Male predominance (M:F = 5:1) was noted with an incidence of five percent of all cases of medulloblastoma (6 out of 120 cases) over a period of 6 years. Primitive neuroectodermal areas were accompanied with areas of "myoid" differentiation, 5 cases showing strap cells. Two cases with epithelial and cartilaginous differentiation were seen. Three cases showed focal melanocytic differentiation, identified only on HMB45 immunostaining. Four cases showed glial differentiation. Neuronal differentiation again was very focally seen in two cases, of which one was identified only by NFP immunostain. Seventh case is included in the study, however it is not considered to calculate incidence as it occurred beyond the period of 6 years of records search.</p> <p>Conclusion</p> <p>Medullomyoblastoma is a rare childhood tumor of cerebellum. Majority of cases reveal divergent differentiation, which are identified with the help of panel of immunostains indicating multi-potential nature of primitive neuroectodermal cells.</p

CPLR and Water Resource Utilization by Livestock Farmers in Different Ecosystems of India

The common property resources comprises of all such resources that are meant for common use of the villagers including all resources. The National Sample Survey Organization (NSSO), 1999 in its report defines CPRs as “Resources accessible to and collectively owned\held\managed by an identifiable community and on which no individual has exclusive property rights are called common property resources”. Over the time, there has been gradual decline in CPRs availability to the villagers. The CPRs play an important role in the livelihood of resource poor farmers. According to NSSO, 1999, the percentage of Common Property Land Resources in total geographical area, Common Property Land Resources per household (ha.), Common Property Land Resources per- capita (ha.) is 15, 0.31 and 0.06 respectively. Components of Common Property Land Resources include Community Pastures and grazing land (23%), Village Forest and woodlots (16%) and Other (61%) and there has been reduction of 19 ha in CPR land during last 5 years (per 1000 ha.). The animal land intensity in India is high with an average land holding size of 1.57 ha supporting nearly 2.94 bovines and 1.14 ovine. This in turn puts pressure on common property resources. In developing countries, common property resources (CPRs) can be an important source of income for certain individuals within households (Maggs and Hoddinott, 1997). The common lands are significant form of natural resource endowment in developing countries, play a vital role in maintaining the ecological balance, and in supporting the rural poor, in eking out their livelihood (Qureshi and Kumar, 1998). The objective of present study is to assess the utilization of CPLRs (Common Property Land Resources) and water resources in different ecosystems of India

Primary papillary epithelial tumour of the sella: expanding the spectrum of TTF-1-positive sellar lesions.

To describe four novel primary epithelial tumours of the sella with papillary architecture and Thyroid Transcription Factor 1 (TTF-1) expression. Paraffin-embedded tissue from the four cases and recurrence of patient 1 was investigated with haematoxylin-eosin, special histochemical stains, immunohistochemistry with a broad panel of antibodies and next-generation sequencing. The ultrastructure of one tumour was studied in tissue retrieved from paraffin. The lesions occurred in three females aged 20, 26 and 42 years and a male aged 49 years. They presented with signs and symptoms secondary to pituitary stalk compression. Preoperative neuroimaging documented mixed solid and cystic, enhancing sellar masses with suprasellar extension. Histologically, the tumours showed thin papillae lined by a single layer of cytokeratin and TTF-1-positive cuboidal and cylindrical cells with mildly atypical nucleus. Next-generation sequencing performed in three cases did not identify any mutations. The main differential diagnosis included metastasis from lung or thyroid carcinoma, extraventricular choroid plexus papilloma and sellar ependymoma. We suggest the descriptive term of primary papillary epithelial tumour of the sella (PPETS) for this entity and propose that it could represent the intracranial equivalent of thyroid-like low-grade nasopharyngeal papillary adenocarcinoma. The cell of origin of PPETS remains undetermined although the intense and ubiquitous expression of TTF-1 may suggest a derivation from the infundibulum or ventricular recess. Our study expands the spectrum of sellar TTF-1-positive tumour and challenges the view that they all derive from pituicytes

Natural resource enhancement through silvipastoral establishment in western Himalayan region

Livestock rearing is an integral part of rural livelihood in the Himalayan region. In spite of abundance of pasture resources, total biomass is insufficient to meet the forage demand. Indiscriminate grazing has caused an alarming decline in the carrying capacity of grazing lands and has caused severe land degradation. This study was carried out with the objective of increasing biomass potential, improving livestock productivity and to arrest environmental degradation. The study was conducted at Ghanetta and Dagoh (Kangra) and Jogindernagar (Mandi) districts in Himachal Pradesh through silvipastoral intervention in conjunction with soil and water conservation measures. Grasslands of the study area were of alluvial–loamy soil with shallow depths. Soil organic matter was 0.72 (Ghanetta), 0.76 (Dagoh) and 0.80% (Jogindernagar). Organic carbon content in the subsurface (15–45 cm) layer was only 30 to 39% of the surface layer (0–15 cm). Chrysopogon (20%) was found the most dominant species, followed by Heteropogon (13%). Average herbage production was 5.613 DM tonne/ha (Ghanetta), 5.458 DM tonne/ha (Dagoh) and 5.233 DM tonne/ha (Jogindernagar). Leaf biomass of different fodder trees ranged from 0.23–0.60 DM tonne/ha (Ghanetta), 0.16–0.51 DM tonne/ha (Dagoh) and 0.21–0.59 DM tonne/ha (Jogindernagar). Maximum biomass of 9.17 DM tonne/ha was obtained at Ghanetta followed by 8.26 DM tonne/ha and 8.23 DM tonne/ha at Dagoh and Jogindernagar, respectively, showing substantial improvement in total biomass potential with silvipastoral intervention in conjunction with soil and water conservation measures

Impact of participatory silvipastoral intervention and soil conservation measures for forage resource enhancement in western Himalaya

Livestock rearing is an important component of rural economy in mid-hills of Himalaya. Inspite of abundant available feed resources, total available biomass is insufficient to sustain the livestock population. Fodder trees particularly in hill ecosystem play an important role in supplementing the fodder requirement especially during the lean period. Information gathered and analysis concludes that Grewia optiva is the most important fodder tree in terms of dominance, palatability and increase in milk yield followed by Artocarpus chaplasha, Morus alba, Bauhinia variegata, Albizia lebbeck and Terminalia alata in Kangra and Mandi districts of Himachal Pradesh. The crude protein content was found highest in Grewia optiva (19.38%) followed by Albizia lebbeck (18.85%), Dendrocalamus hamiltonii (18.01%) and minimum in case of Quercus incana (9.27%). During scarcity of fodder Ficus religiosa is the only fodder tree fed throughout the year. The established silvipasture produced leaf biomass of 2.77 to 6.77 DM kg/tree (Ghanetta), 2.12 to 5.96 DM kg/tree (Jogindernagar) and 2.25 to 6.93 DM kg/tree (Dagoh). Fodder trees planted under silvipastoral system produced average biomass of 1.83 DM tonnes/ha (Ghanetta), 1.49 DM tonnes/ha (Jogindernagar) and 1.66 DM tonnes/ha (Dagoh). Rainfall events of more than 50 mm, though quite less in number (25/165, 24/192 and 17/149), contributed 47.7, 82.3 and 81.7% to the total runoff at Ghanetta, Jogindernagar and Dagoh, respectively. Among the resource conservation measures trenching in combination with vegetative barrier allowed only 8.2% of rain as runoff compared to 41.5% under control (no measure). The silvipasture systems coupled with contour staggered trenches and / or vegetative barrier can effectively arrest the environmental degradation

A study of Smad4, Smad6 and Smad7 in Surgically Resected Samples of Pancreatic Ductal Adenocarcinoma and Their Correlation with Clinicopathological Parameters and Patient Survival

<p>Abstract</p> <p>Background</p> <p>Smad4 is the common mediator of the tumor suppressive functions of TGF-beta. Smad6 and Smad7 are the antagonists of the TGF-beta pathway. This study investigates the differential protein expressions of Smad4, Smad6 and Smad7 in tumor as compared to normal tissue of pancreatic ductal adenocarcinoma (PDAC) and compares them with clinicopathological parameters and patient survival.</p> <p>Results</p> <p>There was a significant difference in protein expressions of Smad4 (p = 0.0001), Smad6 (p = 0.0015) and Smad7 (p = 0.0005) protein in tumor as compared to paired normal samples. Loss of Smad7 expression correlated significantly with tumor size (r = 0.421, p < 0.036) and margin status (r = 0.431; p < .032). Patients with moderate to high Smad4 protein expression had a better survival (median survival = 14.600 ± 2.112 months) than patients with absent or weak Smad4 protein expression (median survival = 7.150 ± 0.662). In addition, advanced disease stage correlated significantly with poor prognosis.</p> <p>Conclusion</p> <p>Loss of Smad4 significantly correlated with poor survival of PDAC patients. In the cases where Smad4 is expressed, Smad6 inhibition is possibly a novel mechanism for Smad4 inactivation. Smad7 has a role in pathobiology of PDAC. Further investigation in the roles of Smad6 and Smad7 would help in the identification of novel therapeutic targets for PDAC.</p

Alterations of tumor suppressor gene p16(INK4a )in pancreatic ductal carcinoma

BACKGROUND: Cell cycle inhibitor and tumor suppressor gene p16 / MTS-1 has been reported to be altered in a variety of human tumors. The purpose of the study was to evaluate primary pancreatic ductal adenocarcinomas for potentially inactivating p16 alterations. METHODS: We investigated the status of p16 gene by polymerase chain reaction (PCR), nonradioisotopic single strand conformation polymorphism (SSCP), DNA sequencing and hypermethylation analysis in 25 primary resected ductal adenocarcinomas. In addition, we investigated p16 protein expression in these cases by immunohistochemistry (IHC) using a monoclonal antibody clone (MS-887-PO). RESULTS: Out of the 25 samples analyzed and compared to normal pancreatic control tissues, the overall frequency of p16 alterations was 80% (20/25). Aberrant promoter methylation was the most common mechanism of gene inactivation present in 52% (13/25) cases, followed by coding sequence mutations in 16% (4/25) cases and presumably homozygous deletion in 12% (3/25) cases. These genetic alterations correlated well with p16 protein expression as complete loss of p16 protein was found in 18 of 25 tumors (72%). CONCLUSION: These findings confirm that loss of p16 function could be involved in pancreatic cancer and may explain at least in part the aggressive behaviour of this tumor type