Conocimientos, Actitudes y Prácticas sobre lactancia materna que poseen las madres que asisten al programa de Vigilancia promoción, crecimiento y desarrollo en el centro salud de Villa Libertad, Managua. Octubre -Diciembre 2015

Se determinaron los conocimientos, actitudes y prácticas sobre lactancia materna que poseen las madres que asisten al programa de Vigilancia, Promoción, Crecimiento y Desarrollo, mediante un estudio descriptivo de corte transversal, probabilístico, realizado en 73 mujeres en rangos de edad de 15 a 42 años, seleccionadas por muestreo aleatorio simple en el Centro de Salud Villa Libertad de la ciudad de Managua en el periodo de Octubre a Diciembre del año 2015. Una vez seleccionada el área de estudio se realizó coordinación con personal de salud y docente a cargo, se identificó el problema objeto de la investigación, se procedió a la elaboración de los objetivos y del instrumento de recolección de datos, posteriormente se hizo la validación del mismo. La entrada al escenario de trabajo para la evaluación se realizó aplicando el formulario conteniendo las variables de acuerdo a cada objetivo específico, los cuales están enmarcados en cuanto a identificar información sociodemográfica, de conocimientos, actitudes y prácticas sobre lactancia materna. Se utilizaron medios y programas informáticos para el proceso de recolección y análisis de datos. Una vez analizada la información se obtuvieron los resultados, donde encontramos que las mujeres estudiadas manifestaron tener adecuados conocimientos sobre lactancia materna obtenidos en la unidad de salud donde han sido atendidas, tienen una actitud favorable, pero la práctica de lactancia materna es inadecuada, porque es mixta debido a que además de la leche materna se implementa el uso de fórmulas lácteas y alimentación complementaria a temprana edad. Palabras Clave: Lactancia Materna, Conocimientos, Actitudes, Prácticas, Fórmul

Method and system for identifying a person using their finger-joint print

Inventor name used in this publication: 张磊Inventor name used in this publication: 张林Inventor name used in this publication: 祝海龙Inventor name used in this publication: 张大鹏Inventor name used in this publication: 骆南Title in Traditional Chinese: 利用指關節紋識別個人身份的方法和系統China2012-2013 > Other Outputs > Patents grantedVersion of Recor

Additional file 5: Table S4. of Differential host gene responses from infection with neurovirulent and partially-neurovirulent strains of Venezuelan equine encephalitis virus

Significantly modulated genes in the brain that were unique to V3000 infection. Genes that were modulated only with V3000 infection in the brain were identified. The list summarizes the commonly modulated genes for each time point studied. Values are expressed as average values of (log2) fold expression for each gene over uninfected controls ± standard error mean (SEM). * P ≤ 0.05. (DOCX 38 kb

Additional file 1: Figure S1. of Differential host gene responses from infection with neurovirulent and partially-neurovirulent strains of Venezuelan equine encephalitis virus

Venn diagram with comparison of gene expression at different time points in a) V3000 infected spleen, b) V3034 infected spleen, c) V3000 infected brain, and d) V3034 infected brain. Significantly modulated genes against each virus at different time points studied were compared in spleen and brain. The genes representing unique or common subsets are shown in the venn diagrams. The numbers in the venn diagrams include both upregulated and downregulated genes. Figure S2. Real-time PCR based validation. Real-time PCR analysis was performed to confirm the microarray results for randomly selected genes a) Stat1, b) Stat2, c) Zfp456, Nt5c2 and NfκB2 post V3000 infection and d) Samd9l post V3000 and V3034 infections. Expression values of all the genes were normalized with the house keeping gene, GAPDH. The results here are representative of 2 biological replicates and 2 technical replicates for each biological replicate. Blue bar: RT-PCR expression level; Red bar: Microarray expression level. Details of primer sets used are given in supplementary table-7. Figure S3. Network analysis of apoptotic genes modulated in response to V3000 and V3034 infections in spleen at 48 h and 72 h pi. Genes significantly modulated against V3000 and V3034 infections at 48 h and 72 h pi in spleen were used to perform in silico network analysis using the Ingenuity Pathway analysis software. Both the viruses resulted in modulation of apoptosis pathway at 48 h and 72 h pi. Figure S4. Network analysis of inflammatory genes modulated in response to V3000 and V3034 infections in spleen at 48 h and 72 h pi. Genes significantly modulated against V3000 and V3034 infections at 48 h and 72 h pi in spleen were used to perform in silico network analysis using the Ingenuity Pathway analysis software. Both the viruses resulted in activation of various immune cells to different degrees at 48 h and 72 h pi as shown above. (DOCX 1842 kb

Additional file 4: Table S3. of Differential host gene responses from infection with neurovirulent and partially-neurovirulent strains of Venezuelan equine encephalitis virus

Significantly modulated genes in the spleen that were unique to V3000 infection. Genes that were modulated only with V3000 infection in the spleen were identified. The list summarizes the commonly modulated genes for each time point studied. Values are expressed as average values of (log2) fold expression for each gene over uninfected controls ± standard error mean (SEM). * P ≤ 0.05. (DOCX 82 kb

Additional file 1: Figure S1. of Eastern equine encephalitis virus in mice I: clinical course and outcome are dependent on route of exposure

Geometric mean viral titer in the liver, heart, kidney, adrenal gland, and pancreas of the groups from BALB/c mice infected with NA EEEV strain FL93-939 (n = 5). Viral titers of tissue homogenate supernatants were determined by standard plaque assay. The limit of detection of the assay is 5 pfu/ml tissue homogenate supernatant. (PPTX 64 kb