Homologous Seminal Plasma and Glutathione Promote Pre-capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa

Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results in retaining structural integrity and progressive motility in post-thawed ram spermatozoa. The aims of this research were (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. Quantitatively and qualitatively, homogenized and pooled ram ejaculates (N=10) were extended with one of the following extenders Control (C)-tris-based, GSH and HSP-free, experimental-1 (E1)-C + GSH 5 mM, experimental-2 (E2)-C + HSP 20 % and experimental-3 (E3)-GSH 5 mM + HSP 20 %. Following thawing, samples were taken at 0-A nd 3-hours from each group (n=10) and were assessed for spermatozoa viability, morphology, and motility pattern. C-0h samples yielded a spermatozoa population with low viability, altered head morphology and highly deviated motility pattern. E3-3h samples yielded spermatozoa with unaffected viability, head morphology and high progressive motility. In conclusion, E3 extender added to cryopreserved-thawed ram spermatozoa is most efficient in obtaining high viability, unaltered head morphology, and progressive motility

Sensitivity of the integrated Welfare Quality® scores to changing values of individual dairy cattle welfare measures

The Welfare Quality((R)) (WQ) protocol for on-farm dairy cattle welfare assessment describes 33 measures and a step-wise method to integrate the outcomes into 12 criteria scores, grouped into four principle scores and into an overall welfare categorisation with four possible levels. The relative contribution of various welfare measures to the integrated scores has been contested. Using a European dataset (491 herds), we investigated: i) variation in sensitivity of integrated outcomes to extremely low and high values of measures, criteria and principles by replacing each actual value with minimum and maximum observed and theoretically possible values; and ii) the reasons for this variation in sensitivity. As intended by the WQ consortium, the sensitivity of integrated scores depends on: i) the observed value of the specific measures/criteria; ii) whether the change was positive/negative; and iii) the relative weight attributed to the measures. Additionally, two unintended factors of considerable influence appear to be side-effects of the complexity of the integration method. Namely: i) the number of measures integrated into criteria and principle scores; and ii) the aggregation method of the measures. Therefore, resource-based measures related to drinkers (which have been criticised with respect to their validity to assess absence of prolonged thirst), have a much larger influence on integrated scores than health-related measures such as 'mortality rate' and 'lameness score'. Hence, the integration method of the WQ protocol for dairy cattle should be revised to ensure that the relative contribution of the various welfare measures to the integrated scores more accurately reflect their relevance for dairy cattle welfare

Uticaj rase, pola, reproduktivnog statusa i izvora nabavke na strahove prouzrokovane bukom u populaciji pasa u Beogradu

The prevalence of canine noise-related fears was studied by the questionnaire method of dogs' owners from different part of Belgrade. Dog-owners (N=147) were chosen from a variety of sources (permissive municipal areas and parks for walking of dogs, dog shows and exhibitions and veterinary surgeries for small animal practice). Owners indicated noise-related fears in 59 dogs (40.14%). Twenty-six (17.69%) of them were mixed breed dogs and 33 (22.45%) were pure breed dogs. Noise-related fears were indicated in 25 females (17.01%) and in 34 (23.13%) males and, in 27 (18.37%) intact and 32 (21.77%) neutered dogs. From 59 dogs with noise-related fears 13 of them (8.84%) were directly purchased from breeders and 46 (31.30%) were obtained from other sources. There was a statistically significant difference (p lt 0.001) in the rate of noise-related fears between the dogs directly purchased from breeders and dogs obtained from other sources.U ovoj studiji je proučavana zastupljenost strahova kod pasa u Beogradu primenom upitnika za njihove vlasnike u različitim delovima Beograda. Vlasnici uključeni u istraživanje (n=147), odabrani su sa različitih mesta kao što su: dozvoljene javne površine i parkovi za šetanje pasa, izložbe pasa I veterinarske ambulante. Vlasnici 59 pasa (40,14%) su naznačili prisustvo strahova usled buke kod svojih ljubimaca. U ovoj grupi pasa bilo je 26 mešanaca (17,69%) i 33 (22,45%) rasna psa. Strah usled buke uočen je kod 25 ženki (17,01%) i 34 mužjaka (23,13%). Broj pasa koji nisu bili sterilisani je bio 27 (18,37%), a sterilisanih 32 (21,77%). Od 59 pasa sa strahovima od buke 13 pasa (8,84%) je bilo kupljeno direktno od odgajivača, dok je 46 pasa (31,30%) nabavljeno iz drugih izvora. Utvrđena je statistički značajna razlika (p lt 0,001) u zastupljenosti strahova usled buke između grupe pasa kupljenih direktno od odgajivača i grupe pasa poreklom iz drugih izvora nabavke

Trained-user opinion about Welfare Quality measures and integrated scoring of dairy cattle welfare

The Welfare Quality (WQ) protocol for on-farm dairy cattle welfare assessment describes 27 measures and a stepwise method for integrating values for these measures into 11 criteria scores, grouped further into 4 principle scores and finally into an overall welfare categorization with 4 levels. We conducted an online survey to examine whether trained users' opinions of the WQ protocol for dairy cattle correspond with the integrated scores (criteria, principles, and overall categorization) calculated according to the WQ protocol. First, the trained users' scores (n = 8-15) for reliability and validity and their ranking of the importance of all measures for herd welfare were compared with the degree of actual effect of these measures on the WQ integrated scores. Logistic regression was applied to identify the measures that affected the WQ overall welfare categorization into the "not classified" or "enhanced" categories for a database of 491 European herds. The smallest multivariate model maintaining the highest percentage of both sensitivity and specificity for the "enhanced" category contained 6 measures, whereas the model for "not classified" contained 4 measures. Some of the measures that were ranked as least important by trained users (e.g., measures relating to drinkers) had the highest influence on the WQ overall welfare categorization. Conversely, measures rated as most important by the trained users (e.g., lameness and mortality) had a lower effect on the WQ overall category. In addition, trained users were asked to allocate criterion and overall welfare scores to 7 focal herds selected from the database (n = 491 herds). Data on all WQ measures for these focal herds relative to all other herds in the database were provided. The degree to which expert scores corresponded to each other, the systematic difference, and the correspondence between median trained-user opinion and the WQ criterion scores were then tested. The level of correspondence between expert scoring and WQ scoring for 6 of the 12 criteria and for the overall welfare score was low. The WQ scores of the protocol for dairy cattle thus lacked correspondence with trained users on the importance of several welfare measures

Sensitivity of the integrated Welfare Quality® scores to changing values of individual dairy cattle welfare measures.

The Welfare Quality® (WQ) protocol for on-farm dairy cattle welfare assessment describes 33 measures and a step-wise method to integrate the outcomes into 12 criteria scores, grouped into four principle scores and into an overall welfare categorisation with four possible levels. The relative contribution of various welfare measures to the integrated scores has been contested. Using a European dataset (491 herds), we investigated: i) variation in sensitivity of integrated outcomes to extremely low and high values of measures, criteria and principles by replacing each actual value with minimum and maximum observed and theoretically possible values; and ii) the reasons for this variation in sensitivity. As intended by the WQ consortium, the sensitivity of integrated scores depends on: i) the observed value of the specific measures/criteria; ii) whether the change was positive/negative; and iii) the relative weight attributed to the measures. Additionally, two unintended factors of considerable influence appear to be side-effects of the complexity of the integration method. Namely: i) the number of measures integrated into criteria and principle scores; and ii) the aggregation method of the measures. Therefore, resource-based measures related to drinkers (which have been criticised with respect to their validity to assess absence of prolonged thirst), have a much larger influence on integrated scores than health-related measures such as ‘mortality rate’ and ‘lameness score’. Hence, the integration method of the WQ protocol for dairy cattle should be revised to ensure that the relative contribution of the various welfare measures to the integrated scores more accurately reflect their relevance for dairy cattle welfare

Review of Legal Requirements on Ammonia and Greenhouse Gases Emissions from Animal Production Buildings in European countries

This review gathers information about the current legal requirements related to the emission of ammonia and greenhouse gases from animal housing in 21 out of the 28 EU countries and in 5 non-EU countries. Overall the review shows that most of the included countries have established substantial procedures to limit ammonia emission and practically no procedures to limit greenhouse gas emission. The review can also be seen as an introduction to the substantial initiatives and decisions taken by the EU in relation to ammonia emission from animal housing, and as a notification on the absence of corresponding initiatives and decisions in relation to greenhouse gases. An EU directive on industrial emissions from 2010 and an implementation decision from 2017 are the main general instruments to reduce ammonia emission from animal housing in the EU. These treaties put limits to ammonia emissions from installations with more than 2000 places for fattening pigs, with more than 750 places for sows and with more than 40,000 places for poultry. As an example, the upper general limit for fattening pigs is 2.6 kg ammonia per animal place per year. This review indicates that the important animal producing countries in the EU have implemented the EU requirements, and, that only a few countries with a large pig population, in relation to their geographical size, have implemented requirements that are stricter than what is required by the EU