Language management in literary events mediated by a Japanese native speakers : Analysis of the interaction in a shopping situation

千葉大学社会文化科学研究科研究プロジェクト報告書第104集『接触場面の言語管理研究vol.3』所

Sensitivity of Five Rapid HIV Tests on Oral Fluid or Finger-Stick Whole Blood: A Real-Time Comparison in a Healthcare Setting

BACKGROUND: Health authorities in several countries recently recommended the expansion of human immunodeficiency virus (HIV) antibody testing, including the use of rapid tests. Several HIV rapid tests are now licensed in Europe but their sensitivity on total blood and/or oral fluid in routine healthcare settings is not known. METHODS AND FINDINGS: 200 adults with documented HIV-1 (n=194) or HIV-2 infection (n=6) were prospectively screened with five HIV rapid tests using either oral fluid (OF) or finger-stick whole blood (FSB). The OraQuick Advance rapid HIV1/2 was first applied to OF and then to FSB, while the other tests were applied to FSB, in the following order: Vikia HIV 1/2, Determine HIV 1-2, Determine HIV-1/2 Ag/Ab Combo and INSTI HIV-1/HIV-2. Tests negative on FSB were repeated on paired serum samples. Twenty randomly selected HIV-seronegative subjects served as controls, and the results were read blindly. Most patients had HIV-1 subtype B infection (63.3%) and most were on antiretroviral therapy (68.5%). Sensitivity was 86.5%, 94.5%, 98.5%, 94.9%, 95.8% and 99% respectively, with OraQuick OF, OraQuick FSB, Vikia, Determine, Determine Ag/Ab Combo and INSTI (p<0.0001). OraQuick was less sensitive on OF than on FSB (p=0.008). Among the six patients with three or more negative tests, two had recent HIV infection and four patients on antiretroviral therapy had undetectable plasma viral load. When patients positive in all the tests were compared with patients who had at least one negative test, only a plasma HIV RNA level<200 cp/ml was significantly associated with a false-negative result (p=0.009). When the 33 rapid tests negative on FSB were repeated on serum, all but six (5 negative with OraQuick, 1 with INSTI) were positive. The sensitivity of OraQuick, Determine and Determine Ag/Ab Combo was significantly better on serum than on FSB (97.5%, p=0.04; 100%, p=0.004; and 100%, p=0.02, respectively). CONCLUSION: When evaluated in a healthcare setting, rapid HIV tests were less sensitive on oral fluid than on finger-stick whole blood and less sensitive on finger-stick whole blood than on serum

Spatial epidemiology and climatic predictors of paediatric dengue infections captured via sentinel site surveillance, Phnom Penh Cambodia 2011–2012

BackgroundDengue is a major contributor to morbidity in children aged twelve and below throughout Cambodia; the 2012 epidemic season was the most severe in the country since 2007, with more than 42,000 reported (suspect or confirmed) cases.MethodsWe report basic epidemiological characteristics in a series of 701 patients at the National Paediatric Hospital in Cambodia, recruited during a prospective clinical study (2011–2012). To more fully explore this cohort, we examined climatic factors using multivariate negative binomial models and spatial clustering of cases using spatial scan statistics to place the clinical study within a larger epidemiological framework.ResultsWe identify statistically significant spatial clusters at the urban village scale, and find that the key climatic predictors of increasing cases are weekly minimum temperature, median relative humidity, but find a negative association with rainfall maximum, all at lag times of 1–6 weeks, with significant effects extending to 10 weeks.ConclusionsOur results identify clustering of infections at the neighbourhood scale, suggesting points for targeted interventions, and we find that the complex interactions of vectors and climatic conditions in this setting may be best captured by rising minimum temperature, and median (as opposed to mean) relative humidity, with complex and limited effects from rainfall. These results suggest that real-time cluster detection during epidemics should be considered in Cambodia, and that improvements in weather data reporting could benefit national control programs by allow greater prioritization of limited health resources to both vulnerable populations and time periods of greatest risk. Finally, these results add to the increasing body of knowledge suggesting complex interactions between climate and dengue cases that require further targeted research

An application of the perpendicular moisture index for the prediction of fire hazard

Various factors contribute to forest fire hazard, and among them vegetation moisture is the one that dictates susceptibility to fire ignition and propagation. The scientific community has developed a number of spectral indices based on remote sensing measurements in the optical domain for the assessment of vegetation equivalent water thickness (EWT), which is defined as the mass of liquid water per unit of leaf surface. However, fire models rely on the live fuel moisture content (LFMC) as a measure of vegetation moisture. LFMC is defined as the ratio of the mass of the liquid water in a fresh leaf over the mass of oven dry leaf, and spectral indices proposed so far fail in capturing LFMC variability. Recently, the perpendicular moisture index (PMI), based on MODIS, was pro-posed to overcome this limitation and provide a direct measure of LFMC. The aim of this research was to understand the potential and limitations of the PMI in predicting fire hazard, towards its ap-plication in a practical context. To this purpose, a data set of more than 7,700 fires recorded in Campania (13,595 km2), Italy, between 2000 and 2008 was compared with PMI derived from MODIS images. Results show that there is no relationship between PMI and fire size, whereas a linear correlation was found between the spectral index and fire rate of spread.Geoscience & Remote SensingCivil Engineering and Geoscience

Technical characteristic of EU-approved HIV screening rapid tests for use on whole blood and/or oral fluid.

<p>Technical characteristic of EU-approved HIV screening rapid tests for use on whole blood and/or oral fluid.</p

Sensitivity of rapid test detection according to the HIV genotype.

<p>*Failure from PCR, serotyping insufficient volume in one sample;</p><p>**HIV-1 Subtype non B: A (3); D (2); F (2); J (1); O (1); CRF01 (3); CRF02 (17); CRF06 (1); CRF19 (1); Recombinant B/CRF02 (1); Complex recombinant (2); Serotyped as non B (24). Fischer exact test, p>0.25.</p