Generation of an integrated Hieracium genomic and transcriptomic resource enables exploration of small RNA pathways during apomixis initiation

Background: Application of apomixis, or asexual seed formation, in crop breeding would allow rapid fixation of complex traits, economizing improved crop delivery. Identification of apomixis genes is confounded by the polyploid nature, high genome complexity and lack of genomic sequence integration with reproductive tissue transcriptomes in most apomicts. Results: A genomic and transcriptomic resource was developed for Hieracium subgenus Pilosella (Asteraceae) which incorporates characterized sexual, apomictic and mutant apomict plants exhibiting reversion to sexual reproduction. Apomicts develop additional female gametogenic cells that suppress the sexual pathway in ovules. Disrupting small RNA pathways in sexual Arabidopsis also induces extra female gametogenic cells; therefore, the resource was used to examine if changes in small RNA pathways correlate with apomixis initiation. An initial characterization of small RNA pathway genes within Hieracium was undertaken, and ovary-expressed ARGONAUTE genes were identified and cloned. Comparisons of whole ovary transcriptomes from mutant apomicts, relative to the parental apomict, revealed that differentially expressed genes were enriched for processes involved in small RNA biogenesis and chromatin silencing. Small RNA profiles within mutant ovaries did not reveal large-scale alterations in composition or length distributions; however, a small number of differentially expressed, putative small RNA targets were identified. Conclusions: The established Hieracium resource represents a substantial contribution towards the investigation of early sexual and apomictic female gamete development, and the generation of new candidate genes and markers. Observed changes in small RNA targets and biogenesis pathways within sexual and apomictic ovaries will underlie future functional research into apomixis initiation in Hieracium. research into apomixis initiation in Hieracium.David S. Rabiger, Jennifer M. Taylor, Andrew Spriggs, Melanie L. Hand, Steven T. Henderson, Susan D. Johnson, Karsten Oelkers, Maria Hrmova, Keisuke Saito, Go Suzuki, Yasuhiko Mukai, Bernard J. Carroll, and Anna M. G. Koltuno

The digital ethical space: towards a transnational documentary ethics, a filmmaker\textquoterights point of view

Over the last decade the integration of web-based technologies into the film supply chain has accelerated a huge shift in the way documentary films are distributed and exhibited. This shift has seen a move away from the previous analogue systems- built on the exclusivity of time and space- into new convergent and transnational methods based on digital systems. This has (had) huge ramifications for all areas of documentary making, none more so than on the issue of documentary ethics. Above all other forms of film (and filmmaking), ethics is one of the key factors that define and distinguish the documentary film. However, much of the ethical frameworks and discourse currently used are from the previous analogue period of distribution and exhibition. Using the making of a feature documentary as a case study, this article explores how the changes in exhibition and distribution have affected the ethical frameworks that have traditionally informed the making of a documentary. The study concludes by demonstrating how current ethical frameworks and safeguarding procedures, undertaken by both documentary makers but also regulatory bodies, need to be rethought in order to respond to the challenges inherent in this transnational landscap

Developing a collaborative methodology for research with community groups

This ‘short communication’ considers four different methodological approaches for ethnographic research projects that engage community groups when the research topic is contested amongst group members. I locate my comments in the context of a proposed project on concepts of gender in the ‘mythopoetic’ men’s movement, and on feminist responses to these concepts; however, many of the points raised are applicable to a range of community projects. I discuss benefits and drawbacks of the following candidate methodologies : participant-observation (in this case, with a male partner researcher who is an ‘insider’) ; participatory-action research; reflexive ethnographic interviewing; and a collaborative documentary that incorporates aspects of video ethnography. A key consideration throughout is the positioning of the researcher and participants. I make a case for the uniquely collaborative potential of video when quite diverse perspectives are part and parcel of a proposed research agenda