The effective role of vitamin D and omega-3 on rats with induced Crohn’s disease

Crohn's disease is an irreversible inflammatory disease, mainly affecting the gastrointestinal tract. The aim of present study was to investigate the effect of dietary vitamin D and omega 3 supplementation on rats with induced Crohn’s disease. Twenty – four adult male albino rats weighing (160 gm) were divided into 4 groups. The 1st group (n=6) was fed on the basal diet. The 2nd to 4th of rats (n=18) were injected with (10 mg/kg for 3 days) of indomethacin at the end of the experiment to induce Crohn’s disease. Groups (3 and 4) were fed on basal diet and given orally 1000/IU/kg/ b.w. of vitamin D and 600 mg/kg/b.w. of omega 3 respectively. At the end of the experimental period (8 weeks), rats were scarified. Biochemical analysis of the tested parameters was determined. The Results indicated that indomethacin Crohn’s rats showed a significant (P< 0.05) decrease in serum albumin, serum Catalase and serum Glutathione peroxidase compared to the negative control one, while serum liver function, MDA, CRP, and immunity proteins were significantly increased.&nbsp

Effect of Psyllium Seeds and Its Husk on Hypercholesterolemic Rats

This study was performed to investigate the outcome of psyllium seeds and their husk on lipid profile for 8 weeks of hypercholesterolemic rats. A number of 36 mature male rats were split into two groups. The first six rats in the study were fed a standard diet (served as – ve control group). The second main group (30 rats) was fed on high cholesterol diet to induce hypercholesterolemia for six weeks, then was divided as follows: Subcategories (1) was fed on a diet with high cholesterol (as a positive control group). Subcategories (2 and 3) were on a diet with high cholesterol supplemented with 2.5% and 5% Psyllium husk powder, respectively. Subcategories (4 and 5) were fed on high cholesterol diet supplemented with 2.5 % and 5% Psyllium seed powder, respectively. The results revealed that liver functions were found to be significantly decreased (P<0.05) by Psyllium husk or seeds at the tested levels. Serum kidney functions and lipid profile were appreciably (P<0.05) decreased. At the same time, HDL-C was noticeably increased by psyllium seeds or husk supplementation at the tested levels in comparison to the positive control group