Monitoring ClimecoVent-systeem in de praktijk - Technisch, teeltkundig en economisch onderzoek naar een energiezuinige kas bij kwekerij Grenspaal B.V.

De tomatentelers Noud en Roy Steegh zijn in januari 2009 op kwekerij Grenspaal te Wellerlooi (L) gestart met een nieuwe kas, voorzien van het ClimecoVent-systeem. Met het ClimecoVent-systeem wordt beoogd zodanig energie te besparen dat de warmtevraag bij een tomatenteelt neerkomt op een aardgasverbruik van 25 tot 30 m3/m2. De productiedoelen liggen gelijk aan die van een standaard tomatenteelt. Gezien het innovatieve en energiezuinige karakter van het ClimecoVent-systeem, heeft Wageningen UR in samenwerking met Climeco het systeem bij kwekerij Grenspaal over een tijdsspanne van ruim een jaar gemonitord in opdracht van het programma “Kas als Energiebron”. Doordat minder gas is gebruikt voor de verwarming van de kas is ook minder CO2 beschikbaar. In combinatie met de schaduwwerking van het extra scherm zou hiermee mogen worden verwacht dat het productieniveau achterblijft, maar dat is niet gebleken

Thermal Enhancement of Cellular Radiation Damage: A Review of Complementary and Synergistic Effects

Hyperthermia treatment can kill mammalian cells in a time and temperature dependent manner. Thermal sensitivity varies extensively among various cell lines in culture and cellular molecular and ultrastructural studies have not resolved which cellular mechanisms underlie thermal cell killing and radiosensitization. The response of cells to heat and radiation are complementary under certain conditions found in human tumors, such as hypoxia, low pH, low nutrient and the S-phase of the cell cycle. Thus, hyperthermia can be used as a complementary treatment modality in the radiotherapy of human cancer. Further studies show that heat treatment causes radiosensitization which is in part associated with the inhibition of repair of radiation damage and is strongly dependent on temperature and on the sequencing. In addition, the conditions such as pH and oxygenation during treatment sequencing can influence the degree of recovery of cells. These factors may be exploited in optimizing therapeutic gain in clinical cancer therapy. Data are shown that transformation from the normal to the tumorigenic state causes random small changes in radiosensitivity and heat sensitivity. Also, treatments combining heat and radiation can lead to increased or decreased transformation in cells depending on the treatment sequence

Het Nieuwe Telen Courgette: Effecten van een nieuw teeltconcept op kasklimaat en energiegebruik

Voor courgette kunnen verschillende onderdelen van Het Nieuwe Telen worden toegepast om te besparen op de warmtevraag. Een beweegbaar scherm is al snel rendabel, en bij vroege teelten is daarnaast ook een vast folie aan te raden. Doordat courgette relatief tolerant is voor een hoge luchtvochtigheid, is een installatie die droge buitenlucht inblaast hier nauwelijks rendabel

Belichting bij sla : economische haalbaarheidsstudie

PT nummer 12521-0

The Effect of Colcemid on the Heat Survival of Mitotic V79 Chinese Hamster Cells

V79 Chinese hamster cells were collected by colcemid addition to study the effect of heat on mitosis. When they were heated at 42°C and 45°C in the presence of 0.06 μg/mL colcemid, cell survival increased over the control samples, which were heated in ordinary medium. Scanning electron microscopy showed that cells heated to 45°C in the presence or absence of colcemid had fewer microvilli on the surface, but they did not have increased bleb formation. Transmission electron microscopy showed that the chromatin was diffuse in the heated cells and the kinetochores were indistinct. The mitochondria in the heated cells were also swollen and contained visible particles

Future observational and modelling needs identified on the basis of the existing shelf data

NOWESP has compiled a vast quantity of existing data from the North-West European Shelf. Such a focused task is without precedence. It is now highly recommended that one, or a few national and international data centres or agencies should be chosen and properly supported by the EU, where all available observational data, including the NOWESP data, are collected, stored, regularly updated by the providers of the data, and made available to the researchers. International agreement must be reached on the quality control procedures and quality standards for data to be stored in these data bases. Proper arrangements should be made to preserve the economic value of the data for their "owners" without compromising use of the data by researchers or duplicating data collecting efforts. The continental shelf data needed are concentration fields of temperature, salinity, nutrients, suspended matter and chlorophyll, which can be called "climatological" fields. For this purpose at least one monthly survey on the whole European shelf is needed at least during five years, with a proper spatial resolution e.g. 1 degree by 1 degree, and at least in those areas where climatological data are now totally lacking. From the modelling point of view an alternative would be the availability of data from sufficiently representative fixed stations on the shelf, with weekly sampling for several years

Synthetic genetic oscillators demonstrate the functional importance of phenotypic variation in pneumococcal-host interactions.

Phenotypic variation is the phenomenon in which clonal cells display different traits even under identical environmental conditions. This plasticity is thought to be important for processes including bacterial virulence, but direct evidence for its relevance is often lacking. For instance, variation in capsule production in the human pathogen Streptococcus pneumoniae has been linked to different clinical outcomes, but the exact relationship between variation and pathogenesis is not well understood due to complex natural regulation. In this study, we use synthetic oscillatory gene regulatory networks (GRNs) based on CRISPR interference (CRISPRi) together with live cell imaging and cell tracking within microfluidics devices to mimic and test the biological function of bacterial phenotypic variation. We provide a universally applicable approach for engineering intricate GRNs using only two components: dCas9 and extended sgRNAs (ext-sgRNAs). Our findings demonstrate that variation in capsule production is beneficial for pneumococcal fitness in traits associated with pathogenesis providing conclusive evidence for this longstanding question