Safety out of control: dopamine and defence

We enjoy a sophisticated understanding of how animals learn to predict appetitive outcomes and direct their behaviour accordingly. This encompasses well-defined learning algorithms and details of how these might be implemented in the brain. Dopamine has played an important part in this unfolding story, appearing to embody a learning signal for predicting rewards and stamping in useful actions, while also being a modulator of behavioural vigour. By contrast, although choosing correct actions and executing them vigorously in the face of adversity is at least as important, our understanding of learning and behaviour in aversive settings is less well developed. We examine aversive processing through the medium of the role of dopamine and targets such as D2 receptors in the striatum. We consider critical factors such as the degree of control that an animal believes it exerts over key aspects of its environment, the distinction between 'better' and 'good' actual or predicted future states, and the potential requirement for a particular form of opponent to dopamine to ensure proper calibration of state values

A Holistic Approach to Design Support for Bio-polymer Based Packaging

This article was published in the Journal of Polymers and the Environment [© Springer] and the definitive version is available at: http://dx.doi.org/10.1007/s10924-012-0545-zThe growing interest in bio-polymers as a packaging material, particularly from companies looking to reduce their environmental footprint, has resulted in wider adoption. Traditionally the selection and specification of packaging materials was based on aesthetic, technical and financial factors, for which established metrics exist. However with bio-polymers, where the primary rationale for their use is environmental, alternative metrics are required. Furthermore, there is a significant strategic element to the decision process that requires a broader range of horizontal and vertical inputs, both within the business and the wider supply chain. It is therefore essential that a holistic approach is taken to the bio-polymer based packaging design process to ensure that the final packaging meets the original strategic intent and overall requirements of the business. Current ecopackaging design tools are generally limited to professional users, such as designers or packaging engineers, and generally provide tactical rather than strategic support. This disconnect, between the need for inclusivity and greater strategic support in holistic design, and the exclusivity and largely tactical support of current eco-design support tools, indicates a clear need for a new decision support tool for sustainable pack design using bio-polymers. This paper proposes a framework for an eco-design decision support tool for bio-polymer based packaging that has been developed using a predominantly qualitative research approach based on reviews, interviews and industrial packaging design experience and is an extension of previously published work. This research investigates further how existing eco-design methods, such as the ‘Balanced Score Card’, can be applied within the tool and how the shortcomings associated with incorporating social and environmental aspects can be partly resolved, through a simplified set of metrics tailored specifically for bio-polymer packaging decisions. The results of this research is a framework for the development of a three tier eco-design tool for bio-polymer packaging that provides decision support at the three critical stages of the design process: strategic fit, Feasibility assessment and concept/ pack development

The dynamics of a pre-mRNA splicing factor in living cells

Pre-mRNA splicing is a predominantly co-transcriptional event which involves a large number of essential splicing factors. Within the mammalian cell nucleus, most splicing factors are concentrated in 20-40 distinct domains called speckles. The function of speckles and the organization of cellular transcription and pre-mRNA splicing in vivo are not well understood. We have investigated the dynamic properties of splicing factors in nuclei of living cells. Here we show that speckles are highly dynamic structures that respond specifically to activation of nearby genes. These dynamic events are dependent on RNA polymerase II transcription, and are sensitive to inhibitors of protein kinases and Ser/Thr phosphatases. When single genes are transcriptionally activated in living cells, splicing factors leave speckles in peripheral extensions and accumulate at the new sites of transcription. We conclude that one function of speckles is to supply splicing factors to active genes. Our observations demonstrate that the interphase nucleus is far more dynamic in nature than previously assumed