Evaluation of different culture systems on the in vitro production of bovine embryos.

The objective of this study was to determine the development potential and quality of in vitro produced bovine embryos cultured individually or in groups. After IVM and IVF, presumptive zygotes were cultured in groups or individually, either in drops or in the modified well of the well (mWOW) system. In Experiment 1, four culture systems were utilized: T1: drop in group (control); T2: mWOW in groups; T3: mWOW individually; and T4: drop individually. Cleavage and blastocyst rates at Days 6, 7 and 8 and total cell number of Day 6 blastocysts were similar (P > 0.05) for all treatments. However, in Day 7 blastocysts, total cell number was lower (P 0.05) for all treatments in the control group. For the vitrified embryos, lower hatching rates (P < 0.05) were observed in the T3 group. In conclusion, embryos cultured in groups in the mWOW system had the same blastocyst rates but better quality (measured by their survival after vitrification) than those cultured individually in the mWOW system

Intracytoplasmic sperm injection in bovine oocytes as potencial reproductive strategy for endangered breeds.

Edited by Arthur da Silva Mariante; Concepta McManus and Antonieta Nassif Salomao

Compaction and dilation rate dependence of stresses in gas-fluidized beds

A particle dynamics-based hybrid model, consisting of monodisperse spherical solid particles and volume-averaged gas hydrodynamics, is used to study traveling planar waves (one-dimensional traveling waves) of voids formed in gas-fluidized beds of narrow cross sectional areas. Through ensemble-averaging in a co-traveling frame, we compute solid phase continuum variables (local volume fraction, average velocity, stress tensor, and granular temperature) across the waves, and examine the relations among them. We probe the consistency between such computationally obtained relations and constitutive models in the kinetic theory for granular materials which are widely used in the two-fluid modeling approach to fluidized beds. We demonstrate that solid phase continuum variables exhibit appreciable ``path dependence'', which is not captured by the commonly used kinetic theory-based models. We show that this path dependence is associated with the large rates of dilation and compaction that occur in the wave. We also examine the relations among solid phase continuum variables in beds of cohesive particles, which yield the same path dependence. Our results both for beds of cohesive and non-cohesive particles suggest that path-dependent constitutive models need to be developed.Comment: accepted for publication in Physics of Fluids (Burnett-order effect analysis added

Effect of different cryoprotectants on the structural preservation of follicles in frozen zebu bovine (Bos indicus) ovarian tissue

Cryopreservation of ovarian tissue is a new and promising technique for germ-line storage. The objective of this study was to evaluate the effect of four cryoprotectants (at two concentrations each) on the preservation of zebu bovine preantral follicles after ovarian cryostorage. Strips of ovarian cortex were cryopreserved using glycerol (GLY; 10 or 20%), ethylene glycol (EG), propanediol (PROH) or dimethylsulphoxide (DMSO; 1.5 or 3 M). In addition, a toxicity test was performed for each cryoprotectant by exposing the ovarian tissue to them without freezing. Tissues were analyzed by histology and transmission electron microscopy. Ovarian tissue frozen in either concentration of DMSO or PROH or in 10% GLY retained a higher percentage of morphologically normal follicles (73–88%) than tissue frozen in 20% GLY or in either concentration of EG (16–52%). In the toxicity test, exposure of tissues to DMSO, PROH or GLY resulted in higher percentages of normal follicles (80–97%) than exposure to EG (49%). Electron microscopy revealed damage to the ultrastructure of follicles frozen in 10% GLY, while follicles cryopreserved in DMSO and PROH at either concentration exhibited normal ultrastructure. In conclusion, DMSO and PROH were the most effective cryoprotectants for zebu ovarian tissue, preserving the structural integrity of somatic and reproductive cells within the ovary