Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion

The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes.Leandro Eugênio Cardamoni Diniz, Claudete de Fátima Ruas, Valdemar de Paula Carvalho, Fabrício Medeiros Torres, Eduardo Augusto Ruas, Melissa de Oliveira Santos, Tumoru Sera and Paulo Maurício Rua

Efeito do número de ordenhas diárias sobre o desempenho reprodutivo de vacas mestiças Holandês-Zebu.

Resumo: Avaliou-se a influência do número de ordenhas diárias sobre o desempenho reprodutivo de 63 vacas mestiças (Holandês-Zebu) com boa condição corporal ao parto, distribuídas em três grupos (G) experimentais: GI ? vacas ordenhadas uma vez ao dia; GII ? vacas ordenhadas duas vezes ao dia; GIII ? vacas ordenhadas alternadamente a cada 14 dias, uma ou duas vezes ao dia. O retorno ao estro (84,3 dias; 81,7 dias e 71,2 dias); a taxa de manifestação de estro (80,9%; 80,90% e 80,90%); o período de serviço (99,0 dias; 114,0 dias e 93,1 dias) e a taxa de gestação até 120 dias pós-parto (66,7%; 57,1%; 66,7%) não foram influenciados (P>0,05) pelo aumento do número de ordenhas diárias. [Effect of the number of daily milking on reproductive performance of crossbred (Holtein x Zebu) cows]. Abstract: This study was carried out to evaluate the influence of the number of daily milking on reproductive performance of 63 crossbred (Holstein-Zebu) cows, with good body condition score at calving, allocatted into three groups (G) according the number of daily milking: GI ? cows milked once a day; GII ? cows milked twice a day; GIII - cows milked alternating once or twice a day, every fourteen days. The return to estrous (84.3 days; 81.7 days and 71.2 days); estrous rates (80.9%; 80.90% e 80.90%); service period (99.0 days; 114.0 days and 93.1 days) and pregnant rates until 120 day postpartum (66.7%; 57.1%; 66.7%) were not influenced (P>0.05) by the number of daily milkin

Babesia bovis: imunidade colostral, primoinfecção e resposta imune em bovinos naturalmente infectados, no Rio Grande do Sul.

O objetivo deste trabalho foi avaliar a imunidade passiva anti-B. bovis, recebida via colostro, a primoinfecção pelo hematozoário e a respectiva resposta imune de terneiros nascidos na primavera no sul do RS

Development of microsatellite markers for identifying Brazilian Coffea arabica varieties

Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be excellent tools for identifying variety and determining genetic relationships. A set of 127 SSR markers was used to analyze genetic similarity in twenty five Coffea arabica varieties. These were composed of nineteen commercially important Brazilians and six interspecific hybrids of Coffea arabica, Coffea canephora and Coffealiberica. The set used comprised 52 newly developed SSR markers derived from microsatellite enriched libraries, 56 designed on the basis of coffee SSR sequences available from public databases, 6 already published, and 13 universal chloroplast microsatellite markers. Only 22 were polymorphic, these detecting 2-7 alleles per marker, an average of 2.5. Based on the banding patterns generated by polymorphic SSR loci, the set of twenty-five coffee varieties were clustered into two main groups, one composed of only Brazilian varieties, and the other of interspecific hybrids, with a few Brazilians. Color mutants could not be separated. Clustering was in accordance with material genealogy thereby revealing high similarity