Potential for macro and micronutrients extraction from tomato plants with different soil water stresses

Different tomato cultivars may present differentiated water needs, making it indispensable to study water demand. Thus, the objective of this work was to evaluate the influence of six water stresses in the soil on the extraction potential of macro and micronutrients in the aerial part of tomato in vegetative stage, cultivar ‘Dominador’ F1, under protected cultivation and drip. The experiment was installed in a greenhouse with a randomized block design with four replications. The treatments consisted of six soil water stresses as indicative of the time of irrigation. The preset stresses were 20, 45, 70, 95, 120 and 145 kPa at 20 cm depth. At 140 days after transplanting, the variables evaluated were: the macro and micronutrient content of shoots. The results showed that to obtain higher levels of macro (P and S) and micronutrients (B and Cu) of the total aerial part of the ‘Dominador’ tomato plant F1, it was obtained at a voltage of 20 kPa, and its value was reduced linearly with the increase of the water tension in the soil

Production of live larvae following in vitro maturation of zebrafish oocytes.

This study aimed to assess the effects of carp pituitary extract (CPE), follicle stimulating hormone (FSH) and luteinizing hormone (LH) on zebrafish oocyte maturation and the ability of these mature oocytes to be fertilized and developed until hatching. Stage III follicles were matured in eight treatments: five concentrations of CPE (16, 32, 48, 64 and 80 μg/mL), one of FSH (0.5 μg/mL), one of LH (0.5 μg/mL), or one combination of FSH (0.5 μg/mL) and LH (0.5 μg/mL). Maturation rates in CPE treatments were 12.8% (16 μg/mL), 24.8% (32 μg/mL), 27.0% (48 μg/mL), 22.7% (64 μg/mL) and 9.6% (80 μg/mL); in FSH was 15.7% (0.5 μg/mL), in LH was 31.8% (0.5 μg/mL) and in FSH (0.5 μg/mL) combined with LH (0.5 μg/mL) it was 50.4%. In vitro fertilization was performed in all treatments; however, only the treatment combining FSH and LH resulted in fertilized oocytes. After maturation using FSH combined with LH, the cleavage rate was 33.3% and hatching rate of live larvae was 20.0%. These results showed that FSH combined with LH was effective in IVM of zebrafish oocyte

Effect of synthesis temperature on crystallinity, morphology and cell viability of nanostructured hydroxyapatite via wet chemical precipitation method: Effect of temperature on hydroxyapatite properties

Hydroxyapatite (HA) is the main natural mineral constituent of bones and is a good alternative for biomedical applications because it is osteoconductive, non-allergenic, and non-carcinogenic, which ensures high biocompatibility. A commonly used method for obtaining hydroxyapatite is the wet route, which is simple and low-cost, produces only water as a final residue, and provides HA with a crystallinity comparable to that of bone tissue, which favors its biocompatibility. Therefore, the objective of this work is to synthesize hydroxyapatite via the wet chemical precipitation method at different temperatures (4°C, 30°C, 50°C, or 70°C) to observe the influence of temperature on crystallinity, morphology, and cytotoxicity. The results of X-ray diffraction show that all syntheses resulted in pure hydroxyapatite, while increasing the temperature led to higher crystallinity (10.6% to 56.2%) and the crystal size was slightly affected. The increase in temperature changed the particle shape from irregular to needle-like. Cell viability was tested by PicoGreen® in VERO cells for samples at concentrations of 30 and 300µg/mL, and the samples synthesized at 4°C, with lower crystallinity, caused less DNA damage to cells compared to the negative control. &nbsp

Electrochemical Oxidation Assessment and Interaction of 2-aminoethanol and N, N-diethylethanamine Propagation in Acidic Medium

Electro�oxidation and inhibitor performance of copper specimens in 1 M hydrochloric acid solu� tion was investigated at room temperature by linear potentiodynamic polarization and gravimetric method in the presence of 2�aminoethanol (A) and N, N�diethylethanamine (D) as an inorganic inhibitor. The effect of the inhibitory concentration on the corrosion behavior of copper was studied over 288 hrs at 298°K. The inhibitory efficiency rise up to 96% for single induced and 98% for synergistic behavior. The adsorption mechanism characteristic was supported by SEM/EDX analysis and adsorption isotherm. From all indica� tion, the inhibitive efficiency of these compounds majorly depends on their molecular structure and concen� tration. The blocking effects of the surface interface were also explained on the basis of the inhibitor active action. 2�aminoethanol and N, N�diethylethanamine inhibits copper in 1 M HCl by strictly affecting both the anodic and cathodic sites. Portion of the surface covered calculated was also found to follow Langmuir adsorption isotherm

Slow freezing versus vitrification for the cryopreservation of zebrafish (Danio rerio) ovarian tissue.

The aim of the present study was to compare the efficiency of vitrification and slow freezing techniques for the cryopreservation of zebrafish ovarian tissue containing immature follicles. In Experiment 1, assessment of cell membrane integrity by trypan blue exclusion staining was used to select the best cryoprotectant solution for each cryopreservation method. Primary growth (PG) oocytes showed the best percentage of membrane integrity (63.5 ± 2.99%) when SF4 solution (2 M methanol + 0.1 M trehalose + 10% egg yolk solution) was employed. The vitrification solution, which presented the highest membrane integrity (V2; 1.5 M methanol + 5.5 M Me2SO + 0.5 M sucrose + 10% egg yolk solution) was selected for Experiment 2. Experiment 2 aimed to compare the vitrification and slow freezing techniques in the following parameters: morphology, oxidative stress, mitochondrial activity, and DNA damage. Frozen ovarian tissue showed higher ROS levels and lower mitochondrial activity than vitrified ovarian tissue. Ultrastructural observations of frozen PG oocytes showed rupture of the plasma membrane, loss of intracellular contents and a large number of damaged mitochondria, while vitrified PG oocytes had intact mitochondria and cell plasma membranes. We conclude that vitrification may be more effective than slow freezing for the cryopreservation of zebrafish ovarian tissue