Controlled deterioration test and use of the Seed Vigor Imaging System (SVIS ® ) to evaluate the physiological potential of crambe seeds

Vigor tests are important tools for verifying the physiological potential of seed lots; however, various aspects can interfere in the consistency of the test results. The search for less subjective procedures has drawn the interest of researchers. The aim of this study was to adapt the methodology of the controlled deterioration test and verify the efficiency of the Seed Vigor Imaging System (SVIS®) to evaluate the vigor of crambe seeds. Initially, seed moisture content was determined and germination and vigor were evaluated in five seed lots. For the controlled deterioration test, the seed moisture content was adjusted to 18%, 20%, and 22%, and the results were interpreted at four and five days after sowing. The controlled deterioration test should be performed with initial adjustment of seed moisture content to 18%, and the germination test should be evaluated on the fifth day after sowing. SVIS® provided information on the vigor index and the length and uniformity of seedling development. Computerized image analysis is effective in evaluating the vigor of crambe seeds, and the uniformity of seedling development parameter offers the most consistent data

Immunophenotyping, plasticity tests and nanotagging of stem cells derived from adipose tissue of wild rodent agouti (Dasyprocta prymnolopha)

ABSTRACT There is a growing interest in the study of unspecialized mesenchymal stem cells, for there are still some discussions about their in vitro behavior. Regenerative medicine is a science undergoing improvement which develops treatments as cell therapy using somatic stem cells. In several studies, adipose tissue is presented as a source of multipotent adult cells that has several advantages over other tissue sources. This study aimed to characterize and evaluate the tagging of mesenchymal stem cells from the agoutis adipose tissue (Dasyprocta prymonolopha), with fluorescent intracytoplasmic nanocrystals. Fibroblast cells were observed, plastic adherent, with extended self-renewal, ability to form colonies, multipotency by differentiation into three lineages, population CD90 + and CD45 - expression, which issued high red fluorescence after the tagging with fluorescent nanocrystals by different paths and cryopreserved for future use. It is possible to conclude that mesenchymal stem cells from agouti adipose tissue have biological characteristics and in vitro behavior that demonstrate its potential for use in clinical tests.</jats:p