Educators’ Resistance to the Technology and Engineering Education Transition

The purpose of the qualitative grounded theory study was to explore why industrial arts educators resisted organizational change to technology and engineering education. An exploratory, grounded theory method was used to identify new theory related to educators’ resistance because the current literature did not provide a theoretical perspective about why industrial arts educators have resisted the change. The sampling frame was derived from a database of 379 secondary technology and engineering education teachers in the state of Kansas, and a sample size of 13 participants was needed to reach theoretical saturation of the phenomenon. The data for the study was collected through observations and face-to-face semi-structured interviews with in-service industrial education teachers. Data collected from the observations and interviews were analyzed using the three phase classic grounded theory coding technique. Data analysis and interpretation resulted in the emergence of three substantive theories related to the study phenomenon: (a) inefficacious transition to technology and engineering education, (b) value for technical learning, and (c) industry demand-based change

Protein Catabolism and Urea Utilization in Water-Deprived and Fasted Ground Squirrels and Rats

The objective of this study was to examine a specific metabolic adaptation, namely, the extent to which protein catabolism was changed in long-term water-deprived and starved Spennophilus lateralis, S. spilosoma, and laboratory rats. It was thought from previous studies that the relative amount of protein and fat catabolism affects water balance and thus determines how long an animal will survive water deprivation. Hibernators may possess unique metabolic flexibility in adapting to water deprivation as they apparently adapt to dehydration when they are hibernating. In the first series of experiments animals· were water deprived for 6 days at 2 seasons and urine volume, urine urea, urine nitrogen and blood urea were determined daily. Urine urea or nitrogen served as the criterion for protein catabolism. In the second experimental series the metabolism of intraperitoneally injected urea was studied to determine the extent of urea nitrogen utilization for nonessential amino acid synthesis. Urine urea and blood urea concentrations were determined 6, 12, and 24 hr postinjection. Late winter and spring water-deprived ground squirrels excreted more urea than late summer and winter animals. Late winter and spring animals were less tolerant (lost weight at a faster rate) of water deprivation. Less tolerance may be accounted for by a faster rate of muscle catabolism relative to adipose tissue catabolism because muscle tissue catabolism provides fewer calories per gram of tissue than catabolism of adipose tissue. In the urea injection experiment only 50% of the injected urea was recovered in the urine by the time blood urea levels returned to control values. Laboratory rats could utilize urea at only half the rate of ground squirrels and rats were also about as tolerant of water deprivation as the least tolerant squirrels of late winter and spring. Urea injection data was interpreted to indicate that renal retention of urea was occurring and urea nitrogen was being utilized in hepatic synthesis of nonessential amino acids. Thus urea nitrogen utilization may have contributed to the decreased rate and amount of protein catabolism and prolonged life in the long-term water-deprived animals. It was concluded that there are seasonal and species differences in tolerance to long-term water deprivation and the attendant starvation. The difference in tolerance may be due to the ability to conserve protein-containing tissue. The ground squirrels exhibited seasonal metabolic flexibility by their changing level of adaptation to water deprivation and starvation

The Inclusion of self-assessment in merit evaluation

The purpose of this survey study was to collect faculty perceptions toward changes made to the faculty merit evaluation process in a college of education at a state comprehensive university. The changes in the evaluation occurred over a two-year period, where a formative rubric and faculty self-assessment were incorporated into the merit instrument. The sampling frame for the study included the college of education faculty members at the university. The data for the study were collected in two different phases using a field-tested online survey that was created to collect the faculty perceptions of the newly developed instrument and process for completing the merit evaluation. The data collected from the surveys were analyzed using nonparametric tests and qualitative textual analysis. Findings from the study indicated favorable perceptions of the new merit evaluation and only a limited number of differences among the demographic groups

Chapter 4: Scholarly Voice and Academic Identity: A Systematic Review of Doctoral Student Agency

This chapter presents the results of a systematic review of the current scholarship into doctoral student agency from a global perspective. In past work, the authors with others have explored doctoral student and research supervisor agency from the perspective of scholar-practitioner agency within the doctoral learning community as well as the post-doctorate practice-based research agenda. This chapter focuses on an analysis of the current scholarship published since 2019 that has continued to examine the aspects of doctoral student voice, agency, and academic identity. Theoretical perspectives were drawn from the scholarship for the theoretical framework of situated learning theory to view how and why doctoral students specifically are able to move from the periphery of the doctoral learning community to center with agency

Ethanol/Naltrexone Interactions at the mu-Opioid Receptor. CLSM/FCS Study in Live Cells

BACKGROUND: Alcoholism is a widespread chronic disorder of complex aetiology with a significant negative impact on the individual and the society. Mechanisms of ethanol action are not sufficiently well understood at the molecular level and the pharmacotherapy of alcoholism is still in its infancy. Our study focuses at the cellular and molecular level on ethanol-induced effects that are mediated through the micro-opioid receptor (MOP) and on the effects of naltrexone, a well-known antagonist at MOP that is used clinically to prevent relapse in alcoholism. METHODOLOGY/PRINCIPAL FINDINGS: Advanced fluorescence imaging by Confocal Laser Scanning Microscopy (CLSM) and Fluorescence Correlation Spectroscopy (FCS) are used to study ethanol effects on MOP and plasma membrane lipid dynamics in live PC12 cells. We observed that relevant concentrations of ethanol (10-40 mM) alter MOP mobility and surface density, and affect the dynamics of plasma membrane lipids. Compared to the action of specific ligands at MOP, ethanol-induced effects show complex kinetics and point to a biphasic underlying mechanism. Pretreatment with naloxone or naltrexone considerably mitigates the effects of ethanol. CONCLUSIONS/SIGNIFICANCE: We suggest that ethanol acts by affecting the sorting of MOP at the plasma membrane of PC12 cells. Naltrexone exerts opposite effects on MOP sorting at the plasma membrane, thereby countering the effects of ethanol. Our experimental findings give new insight on MOP-mediated ethanol action at the cellular and molecular level. We suggest a new hypothesis to explain the well established ethanol-induced increase in the activity of the endogenous opioid system

Discerning Aggregation in Homogeneous Ensembles: A General Description of Photon Counting Spectroscopy in Diffusing Systems

In order to discern aggregation in solutions, we present a quantum mechanical analog of the photon statistics from fluorescent molecules diffusing through a focused beam. A generating functional is developed to fully describe the experimental physical system as well as the statistics. Histograms of the measured time delay between photon counts are fit by an analytical solution describing the static as well as diffusing regimes. To determine empirical fitting parameters, fluorescence correlation spectroscopy is used in parallel to the photon counting. For expedient analysis, we find that the distribution's deviation from a single Poisson shows a difference between two single fluor moments or a double fluor aggregate of the same total intensities. Initial studies were performed on fixed-state aggregates limited to dimerization. However preliminary results on reactive species suggest that the method can be used to characterize any aggregating system.Comment: 30 pages, 5 figure

Proinsulin C-peptide elicits disaggregation of insulin resulting in enhanced physiological insulin effects

Using surface plasmon resonance (SPR) and electrospray mass spectrometry (ESI-MS), proinsulin C-peptide was found to influence insulin-insulin interactions. In SPR with chip-bound insulin, C-peptide mixed with analyte insulin increased the binding, while alone C-peptide did not. A control peptide with the same residues in random sequence had little effect. In ESI-MS, C-peptide lowered the presence of insulin hexamer. The data suggest that C-peptide promotes insulin disaggregation. Insulin/insulin oligomer μM dissociation constants were determined. Compatible with these findings, type 1 diabetic patients receiving insulin and C-peptide developed 66% more stimulation of glucose metabolism than when given insulin alone. A role of C-peptide in promoting insulin disaggregation may be important physiologically during exocytosis of pancreatic β-cell secretory granulae and pharmacologically at insulin injection sites. It is compatible with the normal co-release of C-peptide and insulin and may contribute to the beneficial effect of C-peptide and insulin replacement in type 1 diabetics

High-redshift quasar host galaxies with adaptive optics

We present K band adaptive optics observations of three high-redshift (z ~ 2.2) high-luminosity quasars, all of which were studied for the first time. We also bserved several point spread function (PSF) calibrators, non-simultaneously because of the small field of view. The significant temporal PSF variations on timescales of minutes inhibited a straightforward scaled PSF removal from the quasar images. Characterising the degree of PSF concentration by the radii encircling 20% and 80% of the total flux, respectively, we found that even under very different observing conditions the r_20 vs. r_80 relation varied coherently between individual short exposure images, delineating a well-defined relation for point sources. Placing the quasar images on this relation, we see indications that all three objects were resolved. We designed a procedure to estimate the significance of this result, and to estimate host galaxy parameters, by reproducing the statistical distribution of the individual short exposure images. We find in all three cases evidence for a luminous host galaxy, with a mean absolute magnitude of M_R = -27.0 and scale lengths around ~ 4-12 kpc. Together with a rough estimate of the central black hole masses obtained from C_iv line widths, the location of the objects on the bulge luminosity vs. black hole mass relation is not significantly dfferent from the low-redshift regime, assuming only passive evolution of the host galaxy. Corresponding Eddington luminosities are L_nuc/L_Edd ~ 0.1-0.6.Comment: 17 pages, 16 figures; submitted to Astronomy&Astrophysics. v2: Absolute magnitudes corrected and as a consequence updated two figures, black hole masses and stellar population age estimates in Discussion, but main conclusions remain the same; accepted by A&

C-Peptide Increases Na,K-ATPase Expression via PKC- and MAP Kinase-Dependent Activation of Transcription Factor ZEB in Human Renal Tubular Cells

Replacement of proinsulin C-peptide in type 1 diabetes ameliorates nerve and kidney dysfunction, conditions which are associated with a decrease in Na,K-ATPase activity. We determined the molecular mechanism by which long term exposure to C-peptide stimulates Na,K-ATPase expression and activity in primary human renal tubular cells (HRTC) in control and hyperglycemic conditions.HRTC were cultured from the outer cortex obtained from patients undergoing elective nephrectomy. Ouabain-sensitive rubidium ((86)Rb(+)) uptake and Na,K-ATPase activity were determined. Abundance of Na,K-ATPase was determined by Western blotting in intact cells or isolated basolateral membranes (BLM). DNA binding activity was determined by electrical mobility shift assay (EMSA). Culturing of HRTCs for 5 days with 1 nM, but not 10 nM of human C-peptide leads to increase in Na,K-ATPase α(1)-subunit protein expression, accompanied with increase in (86)Rb(+) uptake, both in normal- and hyperglycemic conditions. Na,K-ATPase α(1)-subunit expression and Na,K-ATPase activity were reduced in BLM isolated from cells cultured in presence of high glucose. Exposure to1 nM, but not 10 nM of C-peptide increased PKCε phosphorylation as well as phosphorylation and abundance of nuclear ERK1/2 regardless of glucose concentration. Exposure to 1 nM of C-peptide increased DNA binding activity of transcription factor ZEB (AREB6), concomitant with Na,K-ATPase α(1)-subunit mRNA expression. Effects of 1 nM C-peptide on Na,K-ATPase α(1)-subunit expression and/or ZEB DNA binding activity in HRTC were abolished by incubation with PKC or MEK1/2 inhibitors and ZEB siRNA silencing.Despite activation of ERK1/2 and PKC by hyperglycemia, a distinct pool of PKCs and ERK1/2 is involved in regulation of Na,K-ATPase expression and activity by C-peptide. Most likely C-peptide stimulates sodium pump expression via activation of ZEB, a transcription factor that has not been previously implicated in C-peptide-mediated signaling. Importantly, only physiological concentrations of C-peptide elicit this effect