58 research outputs found
Intracellular Water Exchange for Measuring the Dry Mass, Water Mass and Changes in Chemical Composition of Living Cells
We present a method for direct non-optical quantification of dry mass, dry density and water mass of single living cells in suspension. Dry mass and dry density are obtained simultaneously by measuring a cell’s buoyant mass sequentially in an H[subscript 2]O-based fluid and a D[subscript 2]O-based fluid. Rapid exchange of intracellular H[subscript 2]O for D[subscript 2]O renders the cell’s water content neutrally buoyant in both measurements, and thus the paired measurements yield the mass and density of the cell’s dry material alone. Utilizing this same property of rapid water exchange, we also demonstrate the quantification of intracellular water mass. In a population of E. coli, we paired these measurements to estimate the percent dry weight by mass and volume. We then focused on cellular dry density – the average density of all cellular biomolecules, weighted by their relative abundances. Given that densities vary across biomolecule types (RNA, DNA, protein), we investigated whether we could detect changes in biomolecular composition in bacteria, fungi, and mammalian cells. In E. coli, and S. cerevisiae, dry density increases from stationary to exponential phase, consistent with previously known increases in the RNA/protein ratio from up-regulated ribosome production. For mammalian cells, changes in growth conditions cause substantial shifts in dry density, suggesting concurrent changes in the protein, nucleic acid and lipid content of the cell.National Cancer Institute (U.S.). Physical Sciences-Oncology Center (U54CA143874)National Institutes of Health (U.S.) (Center for Cell Division Process Grant P50GM6876)National Institutes of Health (U.S.) (Contract R01CA170592)United States. Army Research Office (Institute for Collaborate Biotechnologies Contract W911NF-09-D-0001
Erythrocyte Shape Alteration « In Vitro » : Relationship to Plasma and Cellular Factors Abstract
Dapsone in the management of 'insect bite-like reaction' in a patient with chronic lymphocytic leukaemia
Exaggerated Response to Insect Bites An Unusual Cutaneous Manifestation of Chronic Lymphocytic Leukemia
Exaggerated insect bite reaction exacerbated by a pyogenic infection in a patient with chronic lymphocytic leukaemia
Chronic lymphocytic leukaemia‐associated insect bite‐like reaction responding to ibrutinib, an immunomodulatory Bruton tyrosine kinase inhibitor
Effect Of Zinc On Hemoglobin Binding By Red Blood-cell Membranes
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/62764/1/250251a0.pd
The Discocyte — Echinocyte Transformation of the Human Red Cell : Deformability Characteristics
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