9 research outputs found
The use of high resolution melting (HRM) to map single nucleotide polymorphism markers linked to a covered smut resistance gene in barley
Using an established genetic map, a single gene
conditioning covered smut resistance, Ruh.7H, was mapped
to the telomere region of chromosome 7HS in an Alexis/
Sloop doubled haploid barley population. The closest
marker to Ruh.7H, abg704 was 7.5 cM away. Thirteen loci
on the distal end of 7HS with potential to contain single
nucleotide polymorphisms (SNPs) were identiWed by
applying a comparative genomics approach using rice
sequence data. Of these, one locus produced polymorphic
co-dominant bands of diVerent size while two further loci
contained SNPs that were identiWed using the recently
developed high resolution melting (HRM) technique. Two
of these markers Xanked Ruh.7H with the proximal marker
located 3.8 cM and the distal marker 2.7 cM away. This is
the Wrst report on the application of the HRM technique to
SNP detection and to rapid scoring of known cleaved
ampliWed polymorphic sequence (CAPS) markers in plants.
This simple, precise post-PCR technique should Wnd widespread
use in the Wne-mapping of genetic regions of interest
in complex cereal and other plant genomes