Detection of antibiotics in goats' milk: Comparison of different commercial microbial inhibitor tests developed for the testing of cows' milk

[EN] Nine microbial inhibitor tests validated for cows' milk (BR-AS Special, CMT-Copan Milk Test, Delvotest SP-NT, Delvotest T, Brilliant Black Reduction Test MRL, Charm Blue Yellow II, Charm CowSide II, Eclipse 100, Eclipse 3G) were applied to milk samples from 200 different individual goats. Interpretation of the results was based on visual and instrumental reading. Samples initially testing positive were retested and also tested after a milk pre-treatment (heat treatment, fat removal or fat removal followed by heat treatment). With instrumental reading, most microbial tests commonly used for bovine milk were suitable for goats' milk (specificity 95%), except for BR-AS Special, Charm Blue Yellow II and Delvotest SP-NT. However, visual reading of the results decreased the specificity, with 95% specificity only for CMT-Copan Milk Test, Eclipse 3G and Delvotest T. Fat removal followed by heat treatment proved the most appropriate milk treatment to reduce false positive results for almost all tests.This work forms part of the grant EEBB-I-13-06255 financed by the Ministry of Science and Innovation (Madrid, Spain). The authors are grateful to Analytik in MilchProduktions-und Vertriebs-GmbH, Charm Sciences Inc., DSM Food Specialties, ZEULAB S.L. for their technological support. The authors thank MCC-Vlaanderen and Comite du Lait for the assistance with milk quality and composition analysis and appreciate the cooperation of the commercial dairy goat farms: J. VanWaes (Zaffelare, BE), 't Eikenhof (Lokeren, BE) and 't Leenhof (Zele, BE).Romero Rueda, T.; Van Weyenberg, S.; Molina Pons, MP.; Reybroeck, W. (2016). Detection of antibiotics in goats' milk: Comparison of different commercial microbial inhibitor tests developed for the testing of cows' milk. International Dairy Journal. 62:39-42. https://doi.org/10.1016/j.idairyj.2016.07.004S39426

Analyse du risque posé en santé animale par la présence de l'hydroxyméthylfurfural dans les sirops de nourrissement des abeilles domestiques

This paper discusses an incident that occurred in Belgium during winter 2009-2010, after which many honey bee colonies have been lost. Later analyses showed that these colonies had been fed during the winter with a inverted sugar syrup from sugar beet which had a high concentration of hydroxymethylfurfural (HMF). HMF concentrations ranged from 18.8 ppm to 365.6 ppm. Data from the scientific literature are quite limited, but several authors confirm the harmful effect of HMF on honey bee health. Other elements, however, may have played a role in this apparent mortality such as crystallization of syrups, resulting in unavailability of sugar for the honey bees, and thus the death of them from starvation. Pending the acquisition of new scientific knowledge on the subject, it is recommended to follow good beekeeping practices detailed in this article to minimize the formation of HMF in syrups for honey bee feeding. In addition, a draft action limit corresponding to a maximal concentration of 40 ppm of HMF is proposed in order to control the risk at the producer and distributor of syrups for honey bee feeding level

Determination of somatic cells in milk by solid phase cytometry.

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Inhibition of beta-galactosidase biosynthesis in Escherichia coli by tetracycline residues in milk.

Low levels of tetracyclines found as residues in milk inhibited the biosynthesis of beta-galactosidase in Escherichia coli. To produce the same effect, other antibacterials had to occur in concentrations that were more than 10-fold higher. This relative selectivity was exploited for the development of a screening test for tetracyclines in milk based on a chemiluminometric assay of beta-galactosidase. The method was validated with spiked samples of raw milk and applied to field samples contaminated with tetracyclines