Efeito da complementação alimentar no pós-parto sobre o desempenho produtivo de cabritos Sem Raça Definida (SRD).

A presente pesquisa foi desenvolvida numa fazenda particular em Pe- trolina- PE, no período de maio a novembro de 1995, e objetivou avaliar o efeito da complementação alimentar de matrizes sem raça definida (SRD) sobre o desem- penho produtivo dos cabritos criados em caatinga não cercada. O delineamento experimental utilizado foi inteiramente casualizado com três tratamentos (T1 : pas- tagem nativa (PN); T2: PN + 25% de NDT e T3 PN + 50% de NDT) e 16 repetições cada. A complementação alimentar foi à base de feno de leucena (Leucaena leu- cocephala (Lam.) de Wit) e raspa de mandioca (Manihot esculenta Crantz.) forne- cida a partir do primeiro dia pós-parto. Para os três tratamentos, os pesos médios observados para os cabritos, do nascimento aos 168 dias de idade, foram estatis- ticamente iguais (P>0,05). A taxa geral de mortalidade de crias aos 112 dias de idade foi de 20,41 %o Não foi possível observar efeito dos tratamentos na taxa de sobrevivência das crias, uma vez que, as causas mais comuns de desaparecimen- to dos animais são roubos e predadores

Efeito da complementacao alimentar no pos-parto sobre o peso, a condicao corporal e o intervalo parto-primeiro estro de cabras sem raca definida (SRD).

A pesquisa foi desenvolvida numa fazenda particular em Petrolina-PE, no periodo de maio a novembro de 1995, e objetivou determinar o efeito da complementacao alimentar sobre o peso, condicao corporal e intervalo parto-primeiro estro de 48 cabras sem raca definida (SRD), criadas na caatinga nao cercada, distribuidas em um delineamento experimental inteiramente casualizado com tres tratamentos (T1: pastagem nativa (PN); T2: PN + 25% de NDT e T3: PN = 50% de NDT) e 16 repeticoes cada. A complementacao alimentar foi a base de feno de leucena (Leucaena leucocephala ( Lamb.) de Wit) e raspa de mandioca (Manihot esculenta Crantz.) fornecidas a partir do primeiro dia pos-parto. Para os tres tratamentos, os pesos medios observados ate os 168 dias pos-parto foram estatisticamente iguais e os escores corporais medios, dos 56 aos 168 dias pos-parto, das cabras que receberam complementacao alimentar, foram estatisticamente diferentes daquele observado para as cabras sem complementacao alimentar. Os valores medios do intervalo parto-primeiro estro foram estatisticamente iguais e os porcentuais de cabras que mostraram estro, nos tratamentos que receberam complementacao alimentar (87,50 e 81,25%), tambem foram estatisticamente iguais, porem superiores ao das cabras sem complementacao alimentar (35,71%). Em animais criados em caatinga nao cercada, e possivel melhorar a condicao corporal e aumentar o porcentual de cabras que apresentam estro no pos-parto, atraves da complementacao alimentar no pos-parto

Inmunohistochemical Profile of Solid Cell Nest of Thyroid Gland

It is widely held that solid cell nests (SCN) of the thyroid are ultimobranchial body remnants. SCNs are composed of main cells and C cells. It has been suggested that main cells might be pluripotent cells contributing to the histogenesis of C cells and follicular cells, as well as to the formation of certain thyroid tumors. The present study sought to analyze the immunohistochemical profile of SCN and to investigate the potential stem cell role of SCN main cells. Tissue sections from ten cases of nodular hyperplasia (non-tumor goiter) with SCNs were retrieved from the files of the Hospital Infanta Luisa (Seville, Spain). Parathormone (PTH), calcitonin (CT), thyroglobulin (TG), thyroid transcription factor (TTF-1), galectin 3 (GAL3), cytokeratin 19 (CK 19), p63, bcl-2, OCT4, and SALL4 expression were evaluated by immunohistochemistry. Patient clinical data were collected, and tissue sections were stained with hematoxylin–eosin for histological examination. Most cells stained negative for PTH, CT, TG, and TTF-1. Some cells staining positive for TTF-1 and CT required discussion. However, bcl-2, p63, GAL3, and CK 19 protein expression was detected in main cells. OCT4 protein expression was detected in only two cases, and SALL4 expression in none. Positive staining for bcl-2 and p63, and negative staining for PTH, CT, and TG in SCN main cells are both consistent with the widely accepted minimalist definition of stem cells, thus supporting the hypothesis that they may play a stem cell role in the thyroid gland, although further research will be required into stem cell markers. Furthermore, p63 and GAL-3 staining provides a much more sensitive means of detecting SCNs than staining for carcinoembryonic antigen, calcitonin, or other markers; this may help to distinguish SCNs from their mimics

ESMO recommendations on the standard methods to detect NTRK fusions in daily practice and clinical research

Abstract Background NTRK1, NTRK2 and NTRK3 fusions are present in a plethora of malignancies across different histologies. These fusions represent the most frequent mechanism of oncogenic activation of these receptor tyrosine kinases, and biomarkers for the use of TRK small molecule inhibitors. Given the varying frequency of NTRK1/2/3 fusions, crucial to the administration of NTRK inhibitors is the development of optimal approaches for the detection of human cancers harbouring activating NTRK1/2/3 fusion genes. Materials and methods Experts from several Institutions were recruited by the European Society for Medical Oncology (ESMO) Translational Research and Precision Medicine Working Group (TR and PM WG) to review the available methods for the detection of NTRK gene fusions, their potential applications, and strategies for the implementation of a rational approach for the detection of NTRK1/2/3 fusion genes in human malignancies. A consensus on the most reasonable strategy to adopt when screening for NTRK fusions in oncologic patients was sought, and further reviewed and approved by the ESMO TR and PM WG and the ESMO leadership. Results The main techniques employed for NTRK fusion gene detection include immunohistochemistry, fluorescence in situ hybridization (FISH), RT-PCR, and both RNA-based and DNA-based next generation sequencing (NGS). Each technique has advantages and limitations, and the choice of assays for screening and final diagnosis should also take into account the resources and clinical context. Conclusion In tumours where NTRK fusions are highly recurrent, FISH, RT-PCR or RNA-based sequencing panels can be used as confirmatory techniques, whereas in the scenario of testing an unselected population where NTRK1/2/3 fusions are uncommon, either front-line sequencing (preferentially RNA-sequencing) or screening by immunohistochemistry followed by sequencing of positive cases should be pursued

Metaplastic breast carcinomas exhibit EGFR, but not HER2, gene amplification and overexpression: immunohistochemical and chromogenic in situ hybridization analysis

INTRODUCTION: Metaplastic breast carcinomas constitute a heterogeneous group of neoplasms, accounting for less than 1% of all invasive mammary carcinomas. Approximately 70–80% of metaplastic breast carcinomas overexpress the epidermal growth factor receptor (EGFR). Human epidermal growth factor receptor (HER)2 and EGFR have attracted much attention in the medical literature over the past few years owing to the fact that humanized monoclonal antibodies against HER2 and therapies directed against the extracellular ligand-binding domain or the intracellular tyrosine kinase domain of EGFR have proven successful in treating certain types of human cancer. We investigated whether HER2 and EGFR overexpression was present and evaluated gene amplification in a series of metaplastic breast carcinomas. METHOD: Twenty-five metaplastic breast carcinomas were immunohistochemically analyzed using a monoclonal antibody (31G7) for EGFR and two antibodies for HER2 (Herceptest and CB11) and scored using the Herceptest scoring system. Gene amplification was evaluated by chromogenic in situ hybridization using Zymed Spot-Light EGFR and HER2 amplification probe. The results were evaluated by bright field microscopy under 40× and 63× objective lenses. RESULTS: Nineteen (76%) metaplastic breast carcinomas exhibited EGFR ovexpression, and among these EGFR amplification (defined either by large gene clusters or >5 signals/nucleus in >50% of neoplastic cells) was detected in seven cases (37%): three carcinomas with squamous differentiation and four spindle cell carcinomas. One case exhibited HER2 overexpression of grade 2+ (>10% of cells with weak to moderate complete membrane staining), but HER2 gene amplification was not detected. CONCLUSION: Metaplastic breast carcinomas frequently overexpressed EGFR, which was associated with EGFR gene amplification in one-third of cases. Our findings suggest that some patients with metaplastic breast carcinomas might benefit from novel therapies targeting EGFR. Because most metaplastic breast carcinomas overexpress EGFR without gene amplification, further studies to evaluate EGFR activating mutations are warranted

Evaluation of the Pinus taeda quality using ultrasound.

IUS2-PB1-1

Gas Gain Uniformity Tests performed on Multi Wire Proportional Chambers for the LHCb Muon System

We present the experimental setup and the results of the gas gain uniformity tests performed as part of the quality control of the multiwire proportional chambers produced at CERN for the LHCb Muon system. The test provides a relative gas gain measurement over the whole chamber sensitive area. It is based on the analysis of the pulse height spectrum obtained when the chamber is exposed to {a^241}Am radioactive source. Since the measurement is normalized to the peak of a precise pulse generator, the gain uniformity can also be evaluated among different gas gaps and different chambers