Tipificación SARM en trabajadores asistenciales de las unidades de cuidados intensivos en una institución hospitalaria.

Objective.  To  characterize   microbiologically   MRSA in health care workers of the Intensive Care Units at a hospital institution in the city of Montería. Materials and methods. A longitudinal descriptive study was carried out in 52 workers from two intensive care units; nasal and pharyngeal swabs were taken from each individual, with a periodicity of 15 days / five months. Examined through microbiological methods such as fermentation of mannitol, catalase and coagulase the identification of S. aureus was performed, with the  cefoxitin  disc  by Kirby-Bauer disc-diffusion method the methicillin resistance mediated by the mecA gene was evaluated; antibiotics such as Vancomycin, Erythromycin and Clindamycin were evaluated by this test and confirmed by MicroScan ®. Results. we obtained 88 isolates of Staphylococcus aureus and  21  MRSA,  a  prevalence  of MRSA of 25%. 90.5% of strains Of SARM were recovered from the pharynx, in a greater proportion (50%) in nursing staff. Conclusions. Evidence of MRSA circulation in care staff within a hospital institution generates an alert that allows it to establish containment policies to avoid the dissemination of these strains  and to avoid the appearance of outbreaks or hospital infections associated with this pathogen.Objetivo. Caracterizar microbiológicamente SARM en trabajadores asistenciales de las Unidades de Cuidados Intensivos en una institución hospitalaria de la ciudad de Montería. Materiales y métodos. Se realizó un estudio descriptivo longitudinal, en 52 trabajadores  de dos unidades de cuidado intensivo; se tomaron hisopados nasales y faríngeos a cada individuo, con una periodicidad de 15 días/cinco meses. Mediante pruebas convencionales como fermentación de manitol, catalasa y coagulasa se realizó la identificación de S. aureus, con el disco de cefoxitín por  Kirby-  Bauer se evaluó la resistencia a Meticilina  mediada por el gen mecA; antibióticos como Vancomicina, Eritromicina y Clindamicina fueron evaluados por esta prueba y confirmados por MicroScan ®. Resultados.  se obtuvieron 88 aislamientos de S. aureus y 21 aislamientos SARM,  una  prevalencia  de  portación de SARM del 25%. El 90,5 % de las cepas de SARM fueron recuperadas de la faringe, en mayor proporción (50%) en el personal de enfermería. Conclusiones. La evidencia de su circulación de SARM en el personal asistencial dentro de una institución hospital, genera una alerta que le permite a la misma establecer políticas de contención para evitar la diseminación de estas cepas y evitar la aparición de brotes o infecciones hospitalarias asociadas a este patógeno

Detección de Strongyloides stercoralis en Tierralta, Colombia, utilizando cuatro métodos parasitológicos

Introduction: soil-borne helminth Strongyloides stercoralis is one of the most neglected among neglected tropical diseases. A study was conducted of the presence of S. stercoralis in a village from the department of Córdoba, Colombia, with the purpose of comparing the effectiveness of several diagnostic methods. Methods: stool samples from 262 persons were evaluated. Each sample was examined with four parasitological techniques: direct examination, agar plate culture (APC), the modified Baermann method, and the Harada-Mori technique. Results: S. stercoralis was detected by at least one of the techniques in four of the 262 samples: the Harada-Mori technique detected 2 cases, APC 1 case and direct examination 1 case. The modified Baermann method did not detect any case. No significant differences were found when comparing the techniques. Conclusions: results show that S. stercoralis is not endemic in the village of Córdoba, and that parasitological techniques should be used in combination to improve the quality of diagnosis