A novel antifungal protein from seeds of Sesbania virgata (Cav.) Pers. (Leguminosae-Faboideae)

A novel antifungal protein with a molecular mass around 50 kDa was purified from seeds of Sesbania virgata (Cav.) Pers. using ammonium sulfate fractionation followed by gel filtration on a Sephadex G-75 Superfine (Sigma) column and reverse-phase high performance liquid chromatography on a C8 column. The protein, designated FP1-A, with a novel N-terminal sequence AMVHSPGG(S)FS(P), showed growth inhibitory activity of filamentous fungi Aspergillus niger, Cladosporium cladosporioides, Colletotrichum gloeosporioides and Fusarium solani

A novel antifungal protein from seeds of Sesbania virgata (Cav.) Pers. (Leguminosae-Faboideae)

A novel antifungal protein with a molecular mass around 50 kDa was purified from seeds of Sesbania virgata (Cav.) Pers. using ammonium sulfate fractionation followed by gel filtration on a Sephadex G-75 Superfine (Sigma) column and reverse-phase high performance liquid chromatography on a C8 column. The protein, designated FP1-A, with a novel N-terminal sequence AMVHSPGG(S)FS(P), showed growth inhibitory activity of filamentous fungi Aspergillus niger, Cladosporium cladosporioides, Colletotrichum gloeosporioides and Fusarium solani

Ipomopsin and hymenain, two biscoumarins from seeds of Hymenaea courbaril

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)A new biscoumarin (7-hydroxy-6,6'-dimethoxy-3,7'-dicoumarinyl ether), named hymenain (1) and the known biscoumarin ipomopsin (2) were isolated from the germinated seeds of Hymenaea courbaril var. stilbocarpa. Their structures were elucidated by spectroscopic methods. Scopoletin was also detected in the extracts by TLC and CC-MS analyses. Hymenain and ipomopsin showed direct scavenging effect on a stable free radical, 2,2-diphenyl-1-picryl-hydrazyl (DPPH) with the EC(50) values of 100 and 300 mu M, respectively. (C) 2008 Phytochemical Society of Europe. Published by Elsevier Ltd. All rights reserved.225962Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Science Foundation [NSF-IBN 0335203]DOD-SERDP [SI1388]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP [2004/04477-7, 2005/04139-7]National Science Foundation [NSF-IBN 0335203]DOD-SERDP [SI1388