Acid-adaption by a medic microsymbiont: new insights from the genome of Sinorhizobium medicae WSM419

The poor availability of nitrogen is one of the principal factors limiting global biomass. Legumes are vital components of agricultural systems because of their ability to associate symbiotically with root nodule bacteria (RNB) and subsequently fix atmospheric nitrogen to a form that can be utilised by the plant partner. Furthermore, this symbiotic relationship provides available soil nitrogen for subsequent non-leguminous crops. This RNB-legume interaction is affected by a number of environmental factors. Progressive acidification of agricultural soils is one of the big challenges in agriculture as soil acidity negatively impacts legume productivity. One genus of RNB, Sinorhizobium, is particularly acid-sensitive causing a major reduction in Medicago productivity in acidic soils. Due to the importance of Medic pasture production, alternative strains have been captured, and are still being captured, from the genetic pool that display superior acid tolerance characteristics. This presentation will focus on the acid-tolerant species S. medicae (previously known as S. meliloti) and in particular on the previously used commercial inoculant WSM419

Fixed Point Theorems in Random Fuzzy Metric Space Through Rational Expression

In the present paper we will find some fixed point theorems in random fuzzy metric space, random fuzzy 2-metric space and random fuzzy 3-metric space through rational expression.Also we will find the results forintegral type mapping

Validation of integrated pest management module against insect pests of pigeonpea, Cajanus cajan in Tarai region of Uttarakhand

Experiments on validation of integrated pest management (IPM) module against insect pest of pigeonpea in comparison with the Non-IPM (farmer’s practices) were conducted at N.E.B. Crop Research Centre, G. B. Pant University of Agriculture & Technology, Pantnagar during Kharif 2014 and 2015. Adopted IPM module contained Seed treatment with Trichoderma spp. @10g/kg of seed, Sole crop, Bird perches @ 50/ha, need based insecticides spray (Chlorantraniliprole 18.5SC @ 30 g a. i./ha; Neem soap@10g/lit; Acetamiprid 20SP @ 20 g a. i./ha). The results indicated that minimum population of pod borers (Helicoverpa armigera,Maruca vitrata and podfly) and sucking insects (aphids, jassids, pod bug) was reported in IPM plots and maximum population of insects was observed in Non-IPM plots. Percent insect control over non-IPM was 50.98 % for H. armigera, 44.69 % for M. vitrata and 19.17 % for Maruca webbing were recorded. While, for sucking pest complex, insect control over non-IPM was 51.59 %, 40.36 % and 36.17 % against jassids, aphids and tur pod bug, respectively. Similarly, minimum pod borer damage (6.48 and 7.71 %) was recorded in IPM plots as compared to maximum pod borer damage (8.37 and 8.22 %) in non-IPM plots, respectively during 2014 and 2015. Whereas, pooled grain yield for IPM plots was 1286.5 kg/ha for both seasons as against 888 kg/ha in non-IPM plots with 1:2.89 benefit cost ratio. Hence, It is apparent that studied IPM module was able to increase the yield of pigeonepea with lower cost of production as against non-IPM thus it would be benefiting the farmers

Rapid in situ evolution of nodulating strains for Biserrula pelecinus L. through lateral transfer of a symbiosis island from the original mesorhizobial inoculant

Diverse rhizobia able to nodulate Biserrula pelecinus evolved following in situ transfer of nodA and nifH from an inoculant to soil bacteria. Transfer of these chromosomal genes and the presence of an identical integrase gene adjacent to a Phe tRNA gene in both the inoculant and recipients indicate that there was lateral transfer of a symbiosis island

Some Fixed Point Theorems in Fuzzy Mappings

In this paper we stablished some fixed point and common fixed point theorems for sequence of fuzzymappings,taking rational inequalities which generalized the results of Heilpern [2], Lee, Cho, Lee and Kim [16]Keywords: Fixed point theory, Fuzzy Mappings,Contraction mappings,Upper semi-continuous,common fixedpoint

Relativistic anisotropic charged fluid spheres with varying cosmological constant

Static spherically symmetric anisotropic source has been studied for the Einstein-Maxwell field equations assuming the erstwhile cosmological constant $\Lambda$ to be a space-variable scalar, viz., $\Lambda = \Lambda(r)$. Two cases have been examined out of which one reduces to isotropic sphere. The solutions thus obtained are shown to be electromagnetic in origin as a particular case. It is also shown that the generally used pure charge condition, viz., $\rho + p_r = 0$ is not always required for constructing electromagnetic mass models.Comment: 15 pages, 3 eps figure

New Black Hole Solutions in Brans-Dicke Theory of Gravity

Existence check of non-trivial, stationary axisymmetric black hole solutions in Brans-Dicke theory of gravity in different direction from those of Penrose, Thorne and Dykla, and Hawking is performed. Namely, working directly with the known explicit spacetime solutions in Brans-Dicke theory, it is found that non-trivial Kerr-Newman-type black hole solutions different from general relativistic solutions could occur for the generic Brans-Dicke parameter values -5/2\leq \omega <-3/2. Finally, issues like whether these new black holes carry scalar hair and can really arise in nature and if they can, what the associated physical implications would be are discussed carefully.Comment: 20 pages, no figure, Revtex, version to appear in Phys. Rev.

Uracil DNA glycosylase (UDG) activities in Bradyrhizobium diazoefficiens: characterization of a new class of UDG with broad substrate specificity

Repair of uracils in DNA is initiated by uracil DNA glycosylases (UDGs). Family 1 UDGs (Ung) are the most efficient and ubiquitous proteins having an exquisite specificity for uracils in DNA. Ung are characterized by motifs A (GQDPY) and B (HPSPLS) sequences. We report a novel dimeric UDG, Blr0248 (BdiUng) from Bradyrhizobium diazoefficiens. Although BdiUng contains the motif A (GQDPA), it has low sequence identity to known UDGs. BdiUng prefers single stranded DNA and excises uracil, 5-hydroxymethyl-uracil or xanthine from it. BdiUng is impervious to inhibition by AP DNA, and Ugi protein that specifically inhibits family 1 UDGs. Crystal structure of BdiUng shows similarity with the family 4 UDGs in its overall fold but with family 1 UDGs in key active site residues. However, instead of a classical motif B, BdiUng has a uniquely extended protrusion explaining the lack of Ugi inhibition. Structural and mutational analyses of BdiUng have revealed the basis for the accommodation of diverse substrates into its substrate binding pocket. Phylogenetically, BdiUng belongs to a new UDG family. Bradyrhizobium diazoefficiens presents a unique scenario where the presence of at least four families of UDGs may compensate for the absence of an efficient family 1 homologue

Relativistic Electromagnetic Mass Models: Charged Dust Distribution in Higher Dimensions

Electromagnetic mass models are proved to exist in higher dimensional theory of general relativity corresponding to charged dust distribution. Along with the general proof a specific example is also sited as a supporting candidate.Comment: Latex, 7 pages. Accepted in Astrophysics and Space Scienc

Search for gravitational waves from Scorpius X-1 in the second Advanced LIGO observing run with an improved hidden Markov model

We present results from a semicoherent search for continuous gravitational waves from the low-mass x-ray binary Scorpius X-1, using a hidden Markov model (HMM) to track spin wandering. This search improves on previous HMM-based searches of LIGO data by using an improved frequency domain matched filter, the J-statistic, and by analyzing data from Advanced LIGO's second observing run. In the frequency range searched, from 60 to 650 Hz, we find no evidence of gravitational radiation. At 194.6 Hz, the most sensitive search frequency, we report an upper limit on gravitational wave strain (at 95% confidence) of h095%=3.47×10-25 when marginalizing over source inclination angle. This is the most sensitive search for Scorpius X-1, to date, that is specifically designed to be robust in the presence of spin wandering. © 2019 American Physical Society