Beyond expectations: autism, understanding embarrassment, and the relationship with theory of mind

The ability of high-functioning individuals with autism to understand the complex emotion of embarrassment, and how this relates to an understanding of theory of mind, was investigated. Scenarios involving embarrassing and non-embarrassing situations were presented to a group with autism and three comparison groups. Participants were required to rate the level of embarrassment felt by the protagonist and to justify their choices. The results indicated that those with autism generally gave similar ratings of embarrassment as the comparison groups, but did show significant difficulty with non-embarrassing scenarios, and in providing appropriate justifications for embarrassment. In addition, a significant relationship between scores from false belief tasks and justification scores was found, supporting the proposed link between theory of mind skills and understanding embarrassment. Participants with autism did, however, show a higher than expected understanding of this complex emotion

Characterization of Ex Vivo–Generated Bovine and Human Cartilage by Immunohistochemical, Biochemical, and Magnetic Resonance Imaging Analyses

Osteoarthritis (OA) is a prevalent age-associated disease involving altered chondrocyte homeostasis and cartilage degeneration. The avascular nature of cartilage and the altered chondrocyte phenotype characteristic of OA severely limit the capacity for in vivo tissue regeneration. Cell- and tissue-based repair has the potential to revolutionize treatment of OA, but those approaches have exhibited limited clinical success to date. In this study, we test the hypothesis that bovine and human chondrocytes in a collagen type I scaffold will form hyaline cartilage ex vivo with immunohistochemical, biochemical, and magnetic resonance (MR) endpoints similar to the original native cartilage. Chondrocytes were isolated from 1- to 3-week-old calf knee cartilage or from cartilage obtained from human total knee arthroplasties, suspended in 2.7 mg/mL collagen I, and plated as 300 μL spot cultures with 5 × 106 each. Medium formulations were varied, including the amount of serum, the presence or absence of ascorbate, and treatments with cytokines. Bovine chondrocytes generated metachromatic territorial and interstitial matrix and accumulated type II collagen over time. Type VI collagen was confined primarily to the pericellular region. The ex vivo–formed bovine cartilage contained more chondroitin sulfate per dry weight than native cartilage. Human chondrocytes remained viable and generated metachromatic territorial matrix, but were unable to support interstitial matrix accumulation. MR analysis of ex vivo–formed bovine cartilage revealed evidence of progressively maturing matrix, but MR-derived indices of tissue quality did not reach those of native cartilage. We conclude that the collagen-spot culture model supports formation and maturation of three-dimensional hyaline cartilage from active bovine chondrocytes. Future studies will focus on determining the capacity of human chondrocytes to show comparable tissue formation