Preliminary crystallographic study of peanut peroxidase

The cationic isozyme of peroxidase isolated from suspension cultures of peanut cells is a heme-containing and calcium-dependent glycoprotein having four covalently attached oligosaccharide chains. Attempts were made to crystallize the glycoprotein for X-ray diffraction analysis, and these have met with some success. Crystals have now been grown that are suitable for a full three-dimensional structural analysis. The crystals are thin plates and we have shown them to be of the orthorhombic space group P2(1)2(1)2(1) with a = 48.1, b = 97.2, c = 146.2 A. The crystals diffract to beyond 2.8 A resolution, appear to be stable to lengthy X-ray exposure, and contain two molecules of 40,000 daltons each in the asymmetric unit

The growth of and peroxidase synthesis by two carrot cell lines.

The growth and the synthesis of peroxidase by two carrot lines were examined. The wild cell line WC grows more rapidly than the methotrexate resistant WCA1. However, the amount of protein released into the culture medium is identical for both cell lines while there is an approximately 10-fold greater release of peroxidase by WCA1. Since the antibodies generated against peanut peroxidase apparently react with peroxidase from carrot cells, the specific peroxidase synthesis could be determined. The ratio of newly synthesized protein to newly synthesized peroxidase confirmed the differential release of peroxidase. The results substantiate the view that there is an inverse relationship between growth and peroxidase activity of plant cells

Skin tumour surgery in primary care: do general practitioners need to improve their surgical skills?

Item does not contain fulltextBACKGROUND: Due to a rapid increase in the incidence of skin cancer, it seems inevitable that general practitioners (GPs) will play a larger role in skin cancer care. OBJECTIVES: To assess surgical procedures used by GPs in skin tumour management. METHODS: We performed a retrospective study of 1,898 pathology reports of skin tumours excised by GPs in 2009. RESULTS: In 22.9% no diagnosis was provided on the application form. Mostly, once-off excisions (no preceding biopsy) were performed, 7% of the excised lesions were malignant, and 35% of incisions were incomplete. Excisions in the face and neck region were incomplete in 65.4%; 22% of melanomas were biopsied or shaved. CONCLUSION: This study underlines the difficulties in skin tumour management in primary care. To stimulate adequate resource use, the number of excisions of benign lesions could be lowered, and pretreatment biopsy in non-melanoma skin cancer management should be encouraged. GPs should be aware of their limitations and consider referral of high-risk malignancies. (c) 2015 S. Karger AG, Basel