Freeform Bioprinting of Liver Encapsulated in Alginate Hydrogels Tissue Constructs for Pharmacokinetic Study

An in vitro model that can be realistically and inexpensively used to predict human response to various drug administration and toxic chemical exposure is needed. By fabricating a microscale 3D physiological tissue construct consisting of an array of channels and tissue-embedded chambers, one can selectively develop various biomimicking mammalian tissues for a number of pharmaceutical applications, for example, experimental pharmaceutical screening for drug efficacy and toxicity along with apprehending the disposition and metabolic profile of a candidate drug. This paper addresses issues relating to the development and implementation of a bioprinting process for freeform fabrication of a 3D cell-encapsulated hydrogel-based tissue construct, the direct integration onto a microfluidic device for pharmacokinetic study, and the underlying engineering science for the fabrication of a 3D microscale tissue chamber as well as its application in pharmacokinetic study. To this end, a prototype 3D microfluidic tissue chamber embedded with liver cells encapsulated within a hydrogel matrix construct is bioprinted as a physiological in vitro model for pharmacokinetic study. The developed fabrication processes are further validated and parameters optimized by assessing cell viability and liver cell phenotype, in which metabolic and synthetic liver functions are quantitated.Mechanical Engineerin

Tethered balloon-based measurements of meteorological variables and aerosols

Tethered balloon based measurements of the vertical distributions of temperature, humidity, wind speed, and aerosol concentrations were taken over a 4-hour period beginning at sunrise on June 29, 1976, at Wallops Island, Virginia. Twelve consecutive profiles of each variable were obtained from ground to about 500 meters. These measurements were in conjuction with a noise propagation study on remotely arrayed acoustic range (ROMAAR) at Wallops Flight Center. An organized listing of these vertical soundings is presented. The tethered balloon system configuration utilized for these measurements is described

Damping of antiferromagnetic spin waves by valence fluctuations in the double layer perovskite YBaFe2O5

Inelastic neutron scattering experiments show that spin dynamics in the charge ordered insulating ground state of the double-layer perovskite YBaFe2O5 is well described in terms of eg superexchange interactions. Above the Verwey transition at TV = 308 K, t2g double exchange-type conduction within antiferromagnetic FeO2--BaO--FeO2 double layers proceeds by an electron hopping process that requires a spin flip of the five-fold coordinated Fe ions, costing an energy 5S^2 of approximately 0.1 eV. The hopping process disrupts near-neighbor spin correlations, leading to massive damping of zone-boundary spin waves.Comment: RevTeX, 4 pages, 4 figures, submitted to Phys. Rev. Let

Larkin-Ovchinnikov-Fulde-Ferrell phase in the superconductor (TMTSF)2ClO4: Theory versus experiment

We consider a formation of the Larkin-Ovchinnikov-Fulde-Ferrell (LOFF) phase in a quasi-one-dimensional (Q1D) conductor in a magnetic field, parallel to its conducting chains, where we take into account both the paramagnetic spin-splitting and orbital destructive effects against superconductivity. We show that, due to a relative weakness of the orbital effects in a Q1D case, the LOFF phase appears in (TMTSF)$_2$ClO$_4$ superconductor for real values of its Q1D band parameters. We compare our theoretical calculations with the recent experimental data by Y. Maeno's group [S. Yonezawa et al., Phys. Rev. Lett. \textbf{100}, 117002 (2008)] and show that there is a good qualitative and quantitative agreement between the theory and experimental data.Comment: 4 pages, 1 figur

The effect of drying on timber frame connections post flooding

Timber structures face a unique risk from flooding. Elevations in moisture content due to prolonged wetting will lead to a reduction in the mechanical properties of the building. Despite recent growth of timber construction in the UK and a known increased risk from flooding, little research has been conducted into its effects on light frame timber construction and the most efficacious drying method. This paper presents the results of experiments to determine the optimum drying conditions for light timber frame construction with oriented strand board sheathing after simulated flooding. A series of specimens were wetted for 5 d to simulate flooding before exposure to different environments. The specimens were load tested and values of ultimate strength, yield strength and stiffness were compared to those of un-wetted control specimens. It was found that the optimum drying condition for recovery of the maximum pre-wetting mechanical properties was an environment of 38°C and 40% relative humidity. Although this is an optimal setting there is still a permanent reduction in the mechanical properties of approximately 40% for strengths and 20% for stiffness. Stiffness is dependent on grain orientation; however, strength was found to be independent of grain orientation, as it is governed by the oriented strand board failure

Field Scanner Design for MUSTANG of the Green Bank Telescope

MUSTANG is a bolometer camera for the Green Bank Telescope (GBT) working at a frequency of 90 GHz. The detector has a field of view of 40 arcseconds. To cancel out random emission change from atmosphere and other sources, requires a fast scanning reflecting system with a few arcminute ranges. In this paper, the aberrations of an off-axis system are reviewed. The condition for an optimized system is provided. In an optimized system, as additional image transfer mirrors are introduced, new aberrations of the off-axis system may be reintroduced, resulting in a limited field of view. In this paper, different scanning mirror arrangements for the GBT system are analyzed through the ray tracing analysis. These include using the subreflector as the scanning mirror, chopping a flat mirror and transferring image with an ellipse mirror, and chopping a flat mirror and transferring image with a pair of face-to-face paraboloid mirrors. The system analysis shows that chopping a flat mirror and using a well aligned pair of paraboloids can generate the required field of view for the MUSTUNG detector system, while other systems all suffer from larger off-axis aberrations added by the system modification. The spot diagrams of the well aligned pair of paraboloids produced is only about one Airy disk size within a scanning angle of about 3 arcmin.Comment: 7 pages, 9 figure

Tunable pulse delay and advancement in a coupled nanomechanical resonator-superconducting microwave cavity system

We theoretically study the transmission of a weak probe field under the influence of a strong pump field in a coupled nanomechanical resonator-superconducting microwave cavity system. Using the standard input-output theory, we find that both pulse delay (slow light effect) and advancement (fast light effect) of the probe field can appear in this coupled system provided that we choose the suitable detuning of the pump field from cavity resonance. The magnitude of the delay (advancement) can be tuned continuously by adjusting the power of the pump field. This technique demonstrates great potential in applications including microwave phase shifter and delay line.Comment: 12pages, 3 figure

Dynamical chiral symmetry breaking and a critical mass

On a bounded, measurable domain of non-negative current-quark mass, realistic models of QCD's gap equation can simultaneously admit two inequivalent dynamical chiral symmetry breaking (DCSB) solutions and a solution that is unambiguously connected with the realisation of chiral symmetry in the Wigner mode. The Wigner solution and one of the DCSB solutions are destabilised by a current-quark mass and both disappear when that mass exceeds a critical value. This critical value also bounds the domain on which the surviving DCSB solution possesses a chiral expansion. This value can therefore be viewed as an upper bound on the domain within which a perturbative expansion in the current-quark mass around the chiral limit is uniformly valid for physical quantities. For a pseudoscalar meson constituted of equal mass current-quarks, it corresponds to a mass m_{0^-}~0.45GeV. In our discussion we employ properties of the two DCSB solutions of the gap equation that enable a valid definition of in the presence of a nonzero current-mass. The behaviour of this condensate indicates that the essentially dynamical component of chiral symmetry breaking decreases with increasing current-quark mass.Comment: 9 pages, 7 figures. Minor wording change