The cell cycle program of polypeptide labeling in Chlamydomonas reinhardtii

The cell cycle program of polypeptide labeling in syndhronous cultures of wild-type Chlamydomonas reinhardtii was analyzed by pulse-labeling cells with 35SO4 = or [3H]arginine at different cell cycle stages. Nearly 100 labeled membrane and soluble polypeptides were resolved and studied using one-dimensional sodium dodecyl sulfate (SDS)- polyacrylamide gel electrophoresis. The labeling experiments produced the following results. (a) Total 35SO4 = and [3H]arginine incorporation rates varied independently throughout the cell cycle. 35SO4 = incorporation was highest in the mid-light phase, while [3H]arginine incorporation peaked in the dark phase just before cell division. (b) The relative labeling rate for 20 of 100 polypeptides showed significant fluctuations (3-12 fold) during the cell cycle. The remaining polypeptides were labeled at a rate commensurate with total 35SO4 = or [3H]arginine incorporation. The polypeptides that showed significant fluctuations in relative labeling rates served as markers to identify cell cycle stages. (c) The effects of illumination conditions on the apparent cell cycle stage-specific labeling of polypeptides were tested. Shifting light-grown asynchronous cells to the dark had an immediate and pronounced effect on the pattern of polypeptide labeling, but shifting dark-phase syndhronous cells to the light had little effect. The apparent cell cycle variations in the labeling of ribulose 1,5-biphosphate (RUBP)-carboxylase were strongly influenced by illumination effects. (d) Pulse-chase experiments with light-grown asynchronous cells revealed little turnover or inter- conversion of labeled polypeptides within one cell generation, meaning that major polypeptides, whether labeled in a stage-specific manner or not, do not appear transiently in the cell cycle of actively dividing, light-grown cells. The cell cycle program of labeling was used to analyze effects of a temperature-sensitive cycle blocked (cb) mutant. A synchronous culture of ts10001 was shifted to restrictive temperature before its block point to prevent it from dividing. The mutant continued its cell cycle program of polypeptide labeling for over a cell generation, despite its inability to divide

Cricket bowling: A two-segment Lagrangian model

In this study, a Lagrangian forward solution of the bowling arm in cricket is made using a two-segment rigid body model, coupled with projectile equations for the free flight of the ball. For given initial arm positions and constant joint torques, the equations are solved numerically to determine the ball speed and arm angle at release so that the ball can land on a predetermined position on the pitch. The model was driven with kinematic data from video obtained from an elite bowler. The model can be analysed in order to study the biomechanics of the bowling arm as well as to quantify the effects of changing input parameters on the trajectory and speed of the ball

Modelling foam drainage

Foaming occurs in many distillation and absorption processes. In this paper, two basic building blocks that are needed to model foam drainage and hence foam stability are discussed. The first concerns the flow of liquid from the lamellae to the Plateau borders and the second describes the drainage flows that occur within the borders. The mathematical modelling involves a balance between gravity, diffusion, viscous forces, and varying surface tension effects with or without the presence of monolayers of surfactant. In some cases, mass transfer through the gas-liquid interface also causes foam stabilisation, and must be included. Our model allows us to clarify which mechanisms are most likely to dominate in both the lamellae and Plateau borders and hence to determine their evolution. The model provides a theoretical framework for the prediction of foam drainage and collapse rates. The analysis shows that significant foam stability can arise from small surface tension variations

Measurement of the elastic scattering cross section of neutrons from argon and neon

Background: The most significant source of background in direct dark matter searches are neutrons that scatter elastically from nuclei in the detector's sensitive volume. Experimental data for the elastic scattering cross section of neutrons from argon and neon, which are target materials of interest to the dark matter community, were previously unavailable. Purpose: Measure the differential cross section for elastic scattering of neutrons from argon and neon in the energy range relevant to backgrounds from (alpha,n) reactions in direct dark matter searches. Method: Cross-section data were taken at the Triangle Universities Nuclear Laboratory (TUNL) using the neutron time-of-flight technique. These data were fit using the spherical optical model. Results: The differential cross section for elastic scatting of neutrons from neon at 5.0 and 8.0 MeV and argon at 6.0 MeV was measured. Optical-model parameters for the elastic scattering reactions were determined from the best fit to these data. The total elastic scattering cross section for neon was found to differ by 6% at 5.0 MeV and 13% at 8.0 MeV from global optical-model predictions. Compared to a local optical-model for 40Ar, the elastic scattering cross section was found to differ from the data by 8% at 6.0 MeV. Conclusions: These new data are important for improving Monte-Carlo simulations and background estimates for direct dark matter searches and for benchmarking optical models of neutron elastic scattering from these nuclei

The effect of surfactants on expanding free surfaces

This paper develops a systematic theory for the flow observed in the so-called ``overflowing cylinder'' experiment. The basic phenomenon to be explained is the order of magnitude increase in the surface velocity of a slowly overflowing beaker of water that is caused by the addition of a small amount of soluble surfactant. We perform analyses of (i) an inviscid bulk flow in which diffusion is negligible, (ii) a hydrodynamic boundary layer in which viscous effects become important, (iii) a diffusive boundary layer where diffusion is significant, and by matching these together arrive at a coupled problem for the liquid velocity and surfactant concentration. Our model predicts a relation between surface velocity and surface concentration which is in good agreement with experiment. However a degeneracy in the boundary conditions leaves one free parameter which must be taken from experimental data. We suggest an investigation that may resolve this indeterminacy

Ultra-fast mission analysis routine for Apollo Block 2 environmental control system radiators Final report

Computer program for rapid mission analysis of Apollo Block 2 environmental control system radiator

Orbit-resolved photometry and echelle spectroscopy of the cataclysmic variable ST LMi during a 2007 high state

We present high-resolution echelle spectra and contemporaneous photometry of the polar ST LMi during a high state in 2007 March. Emission lines at Hα, He I λ5876, and He I λ7065 show similar line profiles over orbital phase and have narrow and broad components. These profile changes with phase are very similar to those reported in earlier high-state studies of ST LMi. The radial velocity curves from double Gaussian fits to the line profiles are interpreted as two crossing curves, neither of which is coincident with the orbital motion of the secondary star. We attribute one component to infall motions near the white dwarf and the other to a gas streaming along magnetic field lines connecting the two stars