1,343 research outputs found
Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite
From the standpoints of both basic research and biotechnology, there is considerable interest in reaching a clearer understanding of the diversity of biological mechanisms employed during lignocellulose degradation. Globally, termites are an extremely successful group of wood-degrading organisms and are therefore important both for their roles in carbon turnover in the environment and as potential sources of biochemical catalysts for efforts aimed at converting wood into biofuels. Only recently have data supported any direct role for the symbiotic bacteria in the gut of the termite in cellulose and xylan hydrolysis. Here we use a metagenomic analysis of the bacterial community resident in the hindgut paunch of a wood-feeding âhigherâ Nasutitermes species (which do not contain cellulose-fermenting protozoa) to show the presence of a large, diverse set of bacterial genes for cellulose and xylan hydrolysis. Many of these genes were expressed in vivo or had cellulase activity in vitro, and further analyses implicate spirochete and fibrobacter species in gut lignocellulose degradation. New insights into other important symbiotic functions including H_2 metabolism, CO_2-reductive acetogenesis and N_2 fixation are also provided by this first system-wide gene analysis of a microbial community specialized towards plant lignocellulose degradation. Our results underscore how complex even a 1-ÎŒl environment can be
INVESTIGATION OF THE MECHANISMS OF HEAT EXCHANGER CORROSION IN A MUNICIPAL WASTE INCINERATION PLANT BY ANALYSIS OF THE RAW GAS AND VARIATION OF OPERATING PARAMETERS
The detailed mechanism of high temperature chlorine corrosion, the dominant cause of corrosion in a municipal solid waste incinerator (MSI), has still to be clarified (Schroer, 2002). Upon its way through the boiler the raw gas is subject to various physical and chemical processes and interactions. Of these, sulphation of chlorides is supposed to have the major impact on chlorine corrosion (Neumann, 1997). The physical and chemical mechanisms of corrosion were investigated at a municipal solid waste incinerator. Both, the particulate and gas phase of the flue gas, were chemically and physically analyzed during their way through the boiler, at temperatures from close to 1000 °C down to 200 °C. The raw gas composition was analyzed during normal operation and soot blowing cleaning routine. Additionally, operating parameters of the plant were varied, and deposition processes were evaluated with the aim to find out primary measures to reduce corrosion rates. The particle mass concentration exhibits a bimodal size distribution with maxima at approximately 0.5 ÎŒm â growing by duration of travel â and 100 ÎŒm. First results show that sulphation of the particles can be observed upon travel through the boiler and on the fouling. Sulphur containing additives increased the sulphation of the particles during flight though not to completion
Practice setting and physician influences on judgments of colon cancer treatment by community physicians.
OBJECTIVE: This article compares judgments about the treatment of Dukes' B2 and C colon cancer made by general surgeons to those of internists and family practitioners. Physician and practice variables were specialty, affiliation with a Community Clinical Oncology Program (CCOP) hospital, time in practice, professional centrality (level of participation in cancer information networks), solo practice, and number of colon cancer patients.
DATA COLLECTION METHODS: Data are combined from national probability samples of CCOP- and non-CCOP-affiliated physicians. This study focused on 1,138 internists, family physicians, and general surgeons who participated in decision making for patients diagnosed with Dukes' B2 or C stage colon cancer. Judgments were elicited using brief vignettes.
METHODS OF ANALYSIS: Judgments of adjuvant therapy are classified as (a) consistent with the National Institutes of Health Consensus Conference recommendations (experimental for Dukes' B2, accepted for Dukes' C); (b) accepted treatment for both stages; or (c) experimental for both stages. Multinomial logit analyses were used to examine the association of practice setting and physician characteristics to judgments of treatment.
RESULTS: Surgeons and CCOP-affiliated physicians were more likely to endorse the NIH consensus conference position. Surgeons, younger physicians, and those in group practice were more likely to approve of chemotherapy for both cancer stages. The most common position (chemotherapy experimental) was more likely from nonsurgeons, solo practitioners, and non-CCOP physicians.
CONCLUSION: Physician and practice setting characteristics, including organized structures such as the CCOP, are possible mediating structures that can facilitate dissemination of standards of treatment
Towards a basic endoscopic evaluation of swallowing in acute stroke â identification of salient findings by the inexperienced examiner
Ribonuclease P (RNase P) RNA is converted to a Cd(2+)-ribozyme by a single Rp-phosphorothioate modification in the precursor tRNA at the RNase P cleavage site.
Solidification of small para-H2 clusters at zero temperature
We have determined the ground-state energies of para-H clusters at zero
temperature using the diffusion Monte Carlo method. The liquid or solid
character of each cluster is investigated by restricting the phase through the
use of proper importance sampling. Our results show inhomogeneous
crystallization of clusters, with alternating behavior between liquid and solid
phases up to N=55. From there on, all clusters are solid. The ground-state
energies in the range N=13--75 are established and the stable phase of each
cluster is determined. In spite of the small differences observed between the
energy of liquid and solid clusters, the corresponding density profiles are
significantly different, feature that can help to solve ambiguities in the
determination of the specific phase of H clusters.Comment: 17 pages, accepted for publication in J. Phys. Chem.
Torpor in marsupials: Recent advances
We report new findings about torpor in marsupials with regard to three energy demanding processes: (i) development and growth, (ii) reproduction, and (iii) rewarming. Young marsupials use torpor extensively after they develop endothermy, and torpor is generally deeper and longer than in the same individuals when they reach adult size. Adult marsupials also employ torpor during pregnancy and/or lactation to reduce energy expenditure and perhaps to store fat for later use. Moreover, to enhance the energy-conserving potential of torpor, desert marsupials bask during arousal to minimize energy costs of rewarming. We show that the functions of torpor extend beyond merely reducing energy expenditure during food shortages and that torpor can save substantial amounts of energy even during the rewarming process
Expiratory Muscle Strength Training for Therapy of Pharyngeal Dysphagia in Parkinson's Disease
Background
Pharyngeal dysphagia in Parkinson's disease (PD) is a common and clinically relevant symptom associated with poor nutrition intake, reduced quality of life, and aspiration pneumonia. Despite this, effective behavioral treatment approaches are rare.
Objective
The objective of this study was to verify if 4 week of expiratory muscle strength training can improve pharyngeal dysphagia in the short and long term and is able to induce neuroplastic changes in cortical swallowing processing.
Methods
In this double-blind, randomized, controlled trial, 50 patients with hypokinetic pharyngeal dysphagia, as confirmed by flexible endoscopic evaluation of swallowing, performed a 4-week expiratory muscle strength training. Twenty-five participants used a calibrated (âactiveâ) device, 25 used a sham handheld device. Swallowing function was evaluated directly before and after the training period, as well as after a period of 3âmonth using flexible endoscopic evaluation of swallowing. Swallowing-related cortical activation was measured in 22 participants (active:sham; 11:11) using whole-head magnetencephalography.
Results
The active group showed significant improvement in the flexible endoscopic evaluation of swallowingâbased dysphagia score after 4âweeks and after 3âmonths, whereas in the sham group no significant changes from baseline were observed. Especially, clear reduction in pharyngeal residues was found. Regarding the cortical swallowing network before and after training, no statistically significant differences were found by magnetencephalography examination.
Conclusions
Four-week expiratory muscle strength training significantly reduces overall dysphagia severity in PD patients, with a sustained effect after 3âmonths compared with sham training. This was mainly achieved by improving swallowing efficiency. The treatment effect is probably caused by peripheral mechanisms, as no changes in the cortical swallowing network were identified. © 2021 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Societ
Activation of Innate Immune-Response Genes in Little Brown Bats (Myotis lucifugus) Infected with the Fungus Pseudogymnoascus destructans
Recently bats have been associated with the emergence of diseases, both as reservoirs for several new viral diseases in humans and other animals and, in the northern Americas, as hosts for a devastating fungal disease that threatens to drive several bat species to regional extinction. However, despite these catastrophic events little Information is available on bat
defences or how they interact with their pathogens. Even less is known about the response of bats to infection during torpor or long-term hibernation. Using tissue samples collected at the termination of an experiment to explore the pathogenesis of White Nose Syndrome in Little Brown Bats, we determined if hibernating bats infected with the fungus Pseudogymnoascus destructans could respond to infection by activating genes responsible for innate immune and stress responses. Lesions due to fungal infection and, in some cases, secondary bacterial infections, were restricted to the skin. However, we were unable to obtain sufficient amounts of RNA from these sites. We therefore examined lungs for response at an epithelial surface not linked to the primary site of infection. We found that bats responded to infection with a significant increase in lungs of transcripts for Cathelicidin (an anti-microbial peptide) as well as the immune modulators tumor necrosis factor alpha and interleukins 10 and 23. In conclusion, hibernating bats can respond to experimental P.
destructans infection by activating expression of innate immune response genes.Funding for this study was provided by a Fish and Wildlife Service grant to CRKW, TB and VM and by a Natural Sciences and Engineering Research Council (Discovery) grant to VM and a fellowship within the Postdoc Programme of the DAAD, German Academic Exchange Service (to LW). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.https://journals.plos.org/plosone/article?id=10.1371/journal.pone.011228
- âŠ