The development of experimental chamber and methodological approach for gas-phase hydrogen permeation study

In this work, the development of an experimental chamber and a technique for conducting experiments to study the permeation of hydrogen through metal membranes using an automated complex Gas Reaction Controller are realized. This complex makes it possible to carry out experiments to study the hydrogen permeation with the following parameters: hydrogen inlet pressure up to 50 atmospheres; the temperature in the chamber is from -30 °С to 1000 °С. The sample size is limited to 10 mm. The procedure for calculating the diffusion coefficient, based on the Fick equation, is presented in work. The study of the hydrogen permeation in a titanium alloy Ti-6Al-4V is presented in this research

Establishment and characterization of a bladder cancer cell line with enhanced doxorubicin resistance by mevalonate pathway activation

Resistance to chemotherapy is a major problem in the treatment of urothelial bladder cancer. Several mechanisms have been identified in resistance to doxorubicin by analysis of resistant urothelial carcinoma (UC) cell lines, prominently activation of drug efflux pumps and diminished apoptosis. We have derived a new doxorubicin-resistant cell line from BFTC-905 UC cells, designated BFTC-905DOXO-II. A doxorubicin-responsive green fluorescent protein (GFP) reporter assay indicated that resistance in BFTC905-DOXO-II was not due to increased drug efflux pump activity, whereas caspase-3/7 activation was indeed diminished. Gene expression microarray analysis revealed changes in proapoptotic and antiapoptotic genes, but additionally induction of the mevalonate (cholesterol) biosynthetic pathway. Treatmentwithsimvastatin restored sensitivity of BFTC-905DOXO-II to doxorubicin to that of the parental cell line. Induction of the mevalonate pathway has been reported as a mechanism of chemoresistance in other cancers; this is the first observation in bladder cancer. Combinations of statins with doxorubicin-containing chemotherapy regimens may provide a therapeutic advantage in such case