The effect of sheared diamagnetic flow on turbulent structures generated by the Charney–Hasegawa–Mima equation

The generation of electrostatic drift wave turbulence is modelled by the Charney–Hasegawa–Mima equation. The equilibrium density gradient n0=n0(x) is chosen so that dn0 /dx is nonzero and spatially variable (i.e., v*e is sheared). It is shown that this sheared diamagnetic flow leads to localized turbulence which is concentrated at max(grad n0), with a large dv*e/dx inhibiting the spread of the turbulence in the x direction. Coherent structures form which propagate with the local v*e in the y direction. Movement in the x direction is accompanied by a change in their amplitudes. When the numerical code is initialized with a single wave, the plasma behaviour is dominated by the initial mode and its harmonics

Pedagogical regression through service-learning develops the holistic medical graduate by redefining reciprocity

Pedagogical regression through Service-learning uses dialogue and deliberation as active learning tools. Reciprocity is a consequence rooted in this engagement with diverse communities. The aim of the study was to analyse how reciprocity manifests during service-learning. Data collected from students, Grade 12 learners, radiography facilitators, and conference delegates were analysed. Students indicated that service-learning enhanced their learning through self-discovery and group interactions, but nothing academic was learned from the learners. They experienced personal growth and increased levels of social responsiveness. Grade 12 learners reported no educational exchange with the students. Radiography facilitators agreed that service-learning cultivates reciprocity through application of knowledge and elevates personal growth and social responsiveness in students. Conference delegates said that service-learning enhances the curriculum and cultivates social responsiveness in students. Reciprocity in service-learning is polygonal and needs to be redefined in terms of its equal mutualistic nature, of all communities benefitting from a broadly defined exchange

Exploring Practices Determining School Effectiveness: A Case Study in Selected South African Secondary Schools

When parents choose a school for their children to attend, especially a secondary school, they usually look for an effective school’ so that their children can receive what they regard as a good, quality education. In this process, it is most common that parents evaluate or assess the effectiveness of secondary schools on the basis of the school’s academic results or outputs. But what other factors, apart from academic results, constitute school effectiveness

Effective Mentoring to Improve Job Satisfaction among Beginner Teachers at South African Primary Schools

Teachers leaving the profession before age of retirement is an ongoing problem in schools worldwide. While fewer teachers enter the profession each year, the number of teachers leaving the profession has increased. Many teachers listed lack of job satisfaction as a reason for leaving the education profession, while citing the lack of mentoring as a main cause of job dissatisfaction. This study explores the impact of an effective mentoring program at primary schools in the province of Mpumalanga, South Africa to support and improve job satisfaction among beginner teachers entering the profession. This study follows a quantitative approach, consisting of a Likert-scale questionnaire. The sample of the study was a number of 550 teachers (principals, deputy principals, heads of department, teachers and student teachers) from different races and cultures from 50 randomly selected state and private primary schools in Mpumalanga. After comparing the literature with the respondents' data, the researchers found that the development and implementation of a mentoring program in the province of Mpumalanga would positively impacts beginner teachers' job satisfaction, thus indicating a definite need for such a mentoring program

Resistance or tolerance: an examination of aphid (Sitobion yakini) phloem feeding on Betta and Betta-Dn wheat (Triticum aestivum)

Engineering pest resistance into crops is important. However, the mechanisms of resistance are not clearly understood. In this study, we examined the effects of aphid feeding on Russian wheat aphid-resistant and -susceptible cultivars of wheat (Triticum aestivum L.); Betta-Dn and Betta, respectively, by the grass aphid, Sitobion yakini (Eastop). These cultivars were grown with or without aphid colonies. In each case, we examined the plants specifically for the formation of wound callose associated with the phloem, using aniline blue and fluorescence microscopy. We observed that aphid feeding stimulated the formation of wound callose in the susceptible cultivar, but that callose was comparatively reduced in the resistant cultivar of wheat. In a separate series of experiments, the xenobiotic, 5, 6-carboxyfluorescein diacetate was applied to attached sink leaves, distal to feeding aphids. When leaf segments were examined four hours after application, little evidence of phloem transport of the fluorescent cleavage product, 5, 6-carboxyfluorescein (5, 6-CF), was evident below known aphid-probed sieve tubes. Low levels or absence of 5, 6-CF indicates that either the aphids have successfully redirected sap to themselves, or that the phloem is no longer functional. In contrast, 5, 6-CF transport was evident below sites of aphid probing in Betta-Dn, suggesting that the phloem was still capable of long-distance transport. In addition, callose deposition was reduced in Betta-Dn leaf phloem and it is surmised that transport was not as affected by aphid feeding in the resistant cultivar. This indicates that the ‘resistant’ wheat cultivar may in fact be tolerant to aphid feeding by successfully overcoming the nutrient drain that feeding aphids imposed on the phloem transport system

Towards reconciliation of structure with function in plasmodesmata—who is the gatekeeper?

Whilst the structure of higher plant plasmodesmata was first described by Robards (1963. Desmotubule—a plasmodesmatal substructure. Nature 218, 784), and despite many subsequent intensive investigations, there is still much that remains unclear relating to their ultrastructure and functioning in higher plants. We have examined chemically fixed plant material, and suggest that the conformational changes seen in plasmodesmatal substructure, particularly the deposition of electron-dense extra-plasmodesmal material, is linked to either manipulation of the hormonal balance (as in Avocado fruit), or of osmotic potential in leaf blade material. These changes result in the deposition of β 1,3-glucan (callose) at the neck region of these plasmodesmata. This electron-dense material is deposited at the neck region of plasmodesmata, and forms a collar-like structure. The formation of a collar is shown to be coupled with loss of lucence within the cytoplasmic sleeve. The formation of a collar at the plasmodesmatal orifice thus results in encapsulation and closure of the plasmodesmatal orifice. Closure of the orifice coincides with a loss of electron-lucence and a lack of resolution of the desmotubule. These ultrastructural changes are potentially significant and could contribute to, result in, or assist in the down-regulation of cell to cell trafficking via plasmodesmata

Plasmodesmatal frequency in relation to short-distance transport and phloem loading in leaves of barley (Hordeum vulgare). Phloem is not loaded directly from the symplast

We investigated the phloem loading pathway in barley, by determining plasmodesmatal frequencies at the electron microscope level for both intermediate and small blade bundles of mature barley leaves. Lucifer yellow was injected intercellularly into bundle sheath, vascular parenchyma, and thin-walled sieve tubes. Passage of this symplastically transported dye was monitored with an epifluorescence microscope under blue light. Low plasmodesmatal frequencies endarch to the bundle sheath cells are relatively low for most interfaces terminating at the thin- and thick-walled sieve tubes within this C3 species. Lack of connections between vascular parenchyma and sieve tubes, and low frequencies (0.5% plasmodesmata per μm cell wall interface) of connections between vascular parenchyma and companion cells, as well as the very low frequency of pore-plasmodesmatal connections between companion cells and sieve tubes in small bundles (0.2% plasmodesmata per μm cell wall interface), suggest that the companion cell-sieve tube complex is symplastically isolated from other vascular parenchyma cells in small bundles. The degree of cellular connectivity and the potential isolation of the companion cell-sieve tube complex was determined electrophysiologically, using an electrometer coupled to microcapillary electrodes. The less negative cell potential {average -52 mV) from mesophyll to the vascular parenchyma cells contrasted sharply with the more negative potential (-122.5 mV) recorded for the companion cell-thin-walled sieve tube complex. Although intercellular injection of lucifer yellow clearly demonstrated rapid (0.75 μm s-1) longitudinal and radial transport in the bundle sheath-vascular parenchyma complex, as well as from the bundle sheath through transverse veins to adjacent longitudinal veins, we were neither able to detect nor present unequivocal evidence in support of the symplastic connectivity of the sieve tubes to the vascular parenchyma. Injection of the companion cell-sieve tube complex, did not demonstrate backward connectivity to the bundle sheath. We conclude that the low plasmodesmatal frequencies, coupled with a two-domain electropotential zonation configuration, and the negative transport experiments using lucifer yellow, precludes symplastic phloem loading in barley leaves

The intake and quality of breakfast consumption in adolescents attending public secondary schools in the North West province, South Africa

Objectives: The objectives of the study were to determine the proportion and quality of breakfast intake in adolescents, and to determine the effect of breakfast intake and quality on overall diet quality.Design: A cross-sectional quantitative study design was used.Setting: The setting was seven public secondary schools in Potchefstroom and the surrounding areas in North West province.Subjects: The subjects were randomly selected adolescents in grades 9-11 (n = 244).Outcome measures: A self-administered demographic questionnaire was completed by the adolescents. Single 24-hour recall, using the four-stage multiple-pass method, was conducted to obtain information on breakfast and dietary intake for the day. Breakfast quality was measured by applying a breakfast quality score. The Diet Quality Index Revised was used to assess the quality of the overall diet. Spearman’s rank correlation coefficient was employed to assess an association between the continuous variables. The chi-square or Fisher’s exact test was utilised to assess an association between the categorical variables.Results: The proportion of breakfast intake and skipping was 81% and 19%, respectively. The mean breakfast quality score was moderate (3.1). No significant difference was shown in the diet quality score for the breakfast eaters and skippers. The breakfast quality score was marginally associated with calcium intake (p-value < 0.0001, r = 0.418), phosphorous intake (p-value < 0.0001, r = 0.378) and total diversity score (p-value < 0.0001, r = 0.369).Conclusion: The proportion of breakfast skipping, moderate quality of the breakfast consumed and trend of improved nutrient intake with improved breakfast quality highlights the need for breakfast education and intervention as part of the Integrated School Health Policy.Keywords: adolescent, breakfast, breakfast quality, diet quality, adolescenc

Liver transplantation for non-resectable colorectal liver metastases at a single centre in South Africa: A report of the ethics and regulatory approval process

Non-resectable colorectal liver metastases (CLMs) are generally considered an absolute contraindication for liver transplantation. However, a 2013 Norwegian study transplanted livers in 21 patients with CLMs and reported excellent outcomes. The current article reports on the deliberations of the Wits Human Research Ethics Committee (Medical), which resulted in approval of the first liver transplant for a patient with CLMs in South Africa (SA). Factors considered included the scarcity of donor organs in SA, weighing the best interests of different groups of patients and the requirement for extensive and careful information provision. Originally under the auspices of Section 37 of the World Medical Association Declaration of Helsinki (2013), the protocol has now been formalised as a research project

The microstructure of plasmodesmata in internodal stem tissue of the Saccharum hybrid var. NCo376 : evidence for an apoplasmic loading pathway

The distribution, structure and functional state of plasmodesmata were investigated to gain a clearer understanding of the sucrose transport pathway to the storage parenchyma cells in stem tissue in Saccharum officinarum var. NCo376. Evidence from structural studies on sugarcane stems by electron microscopy indicated that there are numerous plasmodesmata from the vascular bundles through to the storage parenchyma cells in mature stem tissue. Our studies, supported by fluorescence microscopy and iontophoresis, indicate that there are functional plasmodesmata in the phloemunloading pathway from transport phloem tissue to the bundle sheath in Saccharum, which could support symplasmic transport; plasmodesmata outside of the sheath cells in the storage parenchyma appear to be constricted by sphincter-like structures within their neck regions. Staining with Aniline Blue revealed evidence of large callose deposits, which co-localized with plasmodesmatal aggregates in the walls of the storage parenchyma cells. This suggests that the sucrose transport into, and accumulation by, storage parenchyma of mature stem tissue is under apoplasmic control