8,484 research outputs found
Cholinergic cells in the nucleus basalis of mice express the N-methyl-D-aspartate-receptor subunit NR2C and its replacement by the NR2B subunit enhances frontal and amygdaloid acetylcholine levels
It is known that glutamatergic and cholinergic systems interact functionally at the level of the cholinergic basal forebrain. The N-methyl-D-aspartate receptor (NMDA-R) is a multiprotein complex composed of NR1, NR2 and/or NR3 subunits. The subunit composition of NMDA-R of cholinergic cells in the nucleus basalis has not yet been investigated. Here, by means of choline acetyl transferase and NR2B or NR2C double staining, we demonstrate that mice express both the NR2C and NR2B subunits in nucleus basalis cholinergic cells.We generated NR2C-2B mutant mice in which an insertion of NR2B cDNA into the gene locus of the NR2C gene replaced NR2C by NR2B expression throughout the brain. This NR2C-2B mutant was used to examine whether a subunit exchange in cholinergic neurons would affect acetylcholine (ACh) content in several brain structures. We found increased ACh levels in the frontal cortex and amygdala in the brains of NR2C-2B mutant mice. Brain ACh has been implicated in neuroplasticity, novelty-induced arousal and encoding of novel stimuli. We therefore assessed behavioral habituation to novel environments and objects as well as object recognition in NR2C-2B subunit exchange mice. The behavioral analysis did not indicate any gross behavioral alteration in the mutant mice compared with the wildtype mice. Our results show that the NR2C by NR2B subunit exchange in mice affects ACh content in two target areas of the nucleus basalis.
Searching for plasticity in dissociated cortical cultures on multi-electrode arrays
We attempted to induce functional plasticity in dense cultures of cortical cells using stimulation through extracellular electrodes embedded in the culture dish substrate (multi-electrode arrays, or MEAs). We looked for plasticity expressed in changes in spontaneous burst patterns, and in array-wide response patterns to electrical stimuli, following several induction protocols related to those used in the literature, as well as some novel ones. Experiments were performed with spontaneous culture-wide bursting suppressed by either distributed electrical stimulation or by elevated extracellular magnesium concentrations as well as with spontaneous bursting untreated. Changes concomitant with induction were no larger in magnitude than changes that occurred spontaneously, except in one novel protocol in which spontaneous bursts were quieted using distributed electrical stimulation
Homologous and heterologous desensitization of guanylyl cyclase-B signaling in GH3 somatolactotropes
The guanylyl cyclases, GC-A and GC-B, are selective receptors for atrial and C-type natriuretic peptides (ANP and CNP, respectively). In the anterior pituitary, CNP and GC-B are major regulators of cGMP production in gonadotropes and yet mouse models of disrupted CNP and GC-B indicate a potential role in growth hormone secretion. In the current study, we investigate the molecular and pharmacological properties of the CNP/GC-B system in somatotrope lineage cells. Primary rat pituitary and GH3 somatolactotropes expressed functional GC-A and GC-B receptors that had similar EC50 properties in terms of cGMP production. Interestingly, GC-B signaling underwent rapid homologous desensitization in a protein phosphatase 2A (PP2A)-dependent manner. Chronic exposure to either CNP or ANP caused a significant down-regulation of both GC-A- and GC-B-dependent cGMP accumulation in a ligand-specific manner. However, this down-regulation was not accompanied by alterations in the sub-cellular localization of these receptors. Heterologous desensitization of GC-B signaling occurred in GH3 cells following exposure to either sphingosine-1-phosphate or thyrotrophin-releasing hormone (TRH). This heterologous desensitization was protein kinase C (PKC)-dependent, as pre-treatment with GF109203X prevented the effect of TRH on CNP/GC-B signaling. Collectively, these data indicate common and distinct properties of particulate guanylyl cyclase receptors in somatotropes and reveal that independent mechanisms of homologous and heterologous desensitization occur involving either PP2A or PKC. Guanylyl cyclase receptors thus represent potential novel therapeutic targets for treating growth-hormone-associated disorders
Wound-up phase turbulence in the Complex Ginzburg-Landau equation
We consider phase turbulent regimes with nonzero winding number in the
one-dimensional Complex Ginzburg-Landau equation. We find that phase turbulent
states with winding number larger than a critical one are only transients and
decay to states within a range of allowed winding numbers. The analogy with the
Eckhaus instability for non-turbulent waves is stressed. The transition from
phase to defect turbulence is interpreted as an ergodicity breaking transition
which occurs when the range of allowed winding numbers vanishes. We explain the
states reached at long times in terms of three basic states, namely
quasiperiodic states, frozen turbulence states, and riding turbulence states.
Justification and some insight into them is obtained from an analysis of a
phase equation for nonzero winding number: rigidly moving solutions of this
equation, which correspond to quasiperiodic and frozen turbulence states, are
understood in terms of periodic and chaotic solutions of an associated system
of ordinary differential equations. A short report of some of our results has
been published in [Montagne et al., Phys. Rev. Lett. 77, 267 (1996)].Comment: 22 pages, 15 figures included. Uses subfigure.sty (included) and
epsf.tex (not included). Related research in
http://www.imedea.uib.es/Nonlinea
The accretion flow in the discless intermediate polar V2400 Ophiuchi
RXTE observations confirm that the X-ray lightcurve of V2400 Oph is pulsed at
the beat cycle, as expected in a discless intermediate polar. There are no
X-ray modulations at the orbital or spin cycles, but optical line profiles vary
with all three cycles. We construct a model for line-profile variations in a
discless accretor, based on the idea that the accretion stream flips from one
magnetic pole to the other, and show that this accounts for the observed
behaviour over the spin and beat cycles. The minimal variability over the
orbital cycle implies that 1) V2400 Oph is at an inclination of only ~10 deg,
and 2) much of the accretion flow is not in a coherent stream, but is circling
the white dwarf, possibly as a ring of denser, diamagnetic blobs. We discuss
the light this sheds on disc formation in intermediate polars.Comment: 10 pages, 12 figures, To appear in MNRAS, includes low-res figures to
reduce siz
First-Principles Dynamical Coherent-Potential Approximation Approach to the Ferromagnetism of Fe, Co, and Ni
Magnetic properties of Fe, Co, and Ni at finite temperatures have been
investigated on the basis of the first-principles dynamical CPA (Coherent
Potential Approximation) combined with the LDA (Local Density Approximation) +
Hamiltonian in the Tight-Binding Linear Muffintin Orbital (TB-LMTO)
representation. The Hamiltonian includes the transverse spin fluctuation terms.
Numerical calculations have been performed within the harmonic approximation
with 4th-order dynamical corrections. Calculated single-particle densities of
states in the ferromagnetic state indicate that the dynamical effects reduce
the exchange splitting, suppress the band width of the quasi-particle state,
and causes incoherent excitations corresponding the 6 eV satellites. Results of
the magnetization vs temperature curves, paramagnetic spin susceptibilities,
and the amplitudes of local moments are presented. Calculated Curie
temperatures () are reported to be 1930K for Fe, 2550K for Co, and
620K for Ni; for Fe and Co are overestimated by a factor of 1.8,
while in Ni agrees with the experimental result. Effective Bohr
magneton numbers calculated from the inverse susceptibilities are 3.0 (Fe), 3.0 (Co), and 1.6 (Ni), being in
agreement with the experimental ones. Overestimate of in Fe and Co
is attributed to the neglects of the higher-order dynamical effects as well as
the magnetic short range order.Comment: 10 pages, 13 figure
Enhanced Memory for Scenes Presented at Behaviorally Relevant Points in Time
What determines whether a scene is remembered or forgotten? Our results show how visual scenes are encoded into memory at behaviorally relevant points in time
Re-examining the consumption-wealth relationship : the role of model uncertainty
This paper discusses the consumption-wealth relationship. Following the recent influential workof Lettau and Ludvigson [e.g. Lettau and Ludvigson (2001), (2004)], we use data on consumption, assets andlabor income and a vector error correction framework. Key …ndings of their work are that consumption doesrespond to permanent changes in wealth in the expected manner, but that most changes in wealth are transitoryand have no e¤ect on consumption. We investigate the robustness of these results to model uncertainty andargue for the use of Bayesian model averaging. We …nd that there is model uncertainty with regards to thenumber of cointegrating vectors, the form of deterministic components, lag length and whether the cointegratingresiduals a¤ect consumption and income directly. Whether this uncertainty has important empirical implicationsdepends on the researcher's attitude towards the economic theory used by Lettau and Ludvigson. If we workwith their model, our findings are very similar to theirs. However, if we work with a broader set of models andlet the data speak, we obtain somewhat di¤erent results. In the latter case, we …nd that the exact magnitudeof the role of permanent shocks is hard to estimate precisely. Thus, although some support exists for the viewthat their role is small, we cannot rule out the possibility that they have a substantive role to play
Investigation of initiation of gigantic jets connecting thunderclouds to the ionosphere
The initiation of giant electrical discharges called as "gigantic jets"
connecting thunderclouds to the ionosphere is investigated by numerical
simulation method in this paper. Using similarity relations, the triggering
conditions of streamer formation in laboratory situations are extended to form
a criterion of initiation of gigantic jets. The energy source causing a
gigantic jet is considered due to the quasi-electrostatic field generated by
thunderclouds. The electron dynamics from ionization threshold to streamer
initiation are simulated by the Monte Carlo technique. It is found that
gigantic jets are initiated at a height of ~18-24 km. This is in agreement with
the observations. The method presented in this paper could be also applied to
the analysis of the initiation of other discharges such as blue jets and red
sprites.Comment: 12th International Congress on Plasma Physics, 25-29 October 2004,
Nice (France
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