A mixed-mode bending apparatus for delamination testing

A mixed-mode delamination test procedure was developed combining double cantilever beam mode I loading and end notch flexure mode II loading on a split unidirectional laminate. By loading the specimen with a lever, a single applied load simultaneously produces mode I and II bending loads on the specimen. This mixed mode bending (MMB) test was analyzed using both finite element procedures and beam theory to calculate the mode I and II components of strain energy release rate, G sub I and G sub II, respectively. The analyses showed that a wide range of G sub I/G sub II ratios could be produced by varying the applied load position on the loading lever. As the delamination extended, the G sub I/G sub II ratios varied by less than 5 percent. The simple beam theory equations were modified to account for the elastic interaction between the two arms of the specimen and to account for shear deformations. The resulting equations agreed closely with the finite element results and provide a basis for selection of G sub I/G sub II test ratios and a basis for computing the mode I and II components of measured delamination toughness. The MMB specimen analysis and test procedures were demonstrated using unidirectional laminates

Summary of a flight-test evaluation of the CL-84 tilt-wing V/STOL aircraft

Flight test evaluation of second generation CL-84 tilt-wing aircraft in hover, transition, and cruise mode

Engineering tyrosine-based electron flow pathways in proteins: The case of aplysia myoglobin

Tyrosine residues can act as redox cofactors that provide an electron transfer ("hole-hopping") route that enhances the rate of ferryl heme iron reduction by externally added reductants, for example, ascorbate. Aplysia fasciata myoglobin, having no naturally occurring tyrosines but 15 phenylalanines that can be selectively mutated to tyrosine residues, provides an ideal protein with which to study such through-protein electron transfer pathways and ways to manipulate them. Two surface exposed phenylalanines that are close to the heme have been mutated to tyrosines (F42Y, F98Y). In both of these, the rate of ferryl heme reduction increased by up to 3 orders of magnitude. This result cannot be explained in terms of distance or redox potential change between donor and acceptor but indicates that tyrosines, by virtue of their ability to form radicals, act as redox cofactors in a new pathway. The mechanism is discussed in terms of the Marcus theory and the specific protonation/deprotonation states of the oxoferryl iron and tyrosine. Tyrosine radicals have been observed and quantified by EPR spectroscopy in both mutants, consistent with the proposed mechanism. The location of each radical is unambiguous and allows us to validate theoretical methods that assign radical location on the basis of EPR hyperfine structure. Mutation to tyrosine decreases the lipid peroxidase activity of this myoglobin in the presence of low concentrations of reductant, and the possibility of decreasing the intrinsic toxicity of hemoglobin by introduction of these pathways is discussed. © 2012 American Chemical Society

Impact of Grazing Management Strategies on Carbon Sequestration in a Semi-Arid Rangeland, USA

The effects of 12 years of grazing management strategies on carbon (C) distribution and sequestration were assessed on a semi-arid mixed-grass prairie in Wyoming, USA. Five grazing treatments were evaluated: non-grazed exclosures; continuous, season-long grazing at a light (22 steer-days ha-1) stocking rate; and, rotationally-deferred, short-duration rotation, and continuous, season-long grazing, all three at a heavy stocking rate (59 steer-days ha-1). Non-grazed exclosures exhibited a large buildup of dead plant material (72% of total aboveground plant matter) and forb biomass represented a large component (35%) of the plant community. Stocking rate, but not grazing strategy, changed plant community composition and decreased surface litter. Light grazing decreased forbs and increased cool-season mid-grasses, resulting in a highly diversified plant community and the highest total production of grasses. Heavy grazing increased warm-season grasses at the expense of the cool-season grasses, which decreased total forage production and opportunity for early season grazing. Compared to the exclosures, all grazing treatments resulted in significantly higher levels of C (6000-9000 kg ha-1) in the surface 15 cm of the soil. Higher levels of soil C with grazing are likely the result of faster litter decomposition and recycling, and redistribution of C within the 0-60 cm plant-soil system. Grazing at an appropriate stocking rate had beneficial effects on plant composition, forage production, and soil C sequestration. Without grazing, deterioration of the plant-soil system is indicated

Lessons Learned from Recent Failure and Incident Investigations of Composite Structures

During the past few decades, NASA Langley Research Center (LaRC) has supported several large-scale failure and incident investigations and numerous requests for engineering consultations. Although various extenuating circumstances contributed to each of these incidents, in all cases, the failure resulted from accumulation and/or propagation of damage that reduced the load carrying capability of the structure to a level below that which was needed to sustain structural loads. A brief overview of various failure and incident investigations supported by LaRC, including some of the computational and experimental methodologies that have been applied, is presented. An important outcome of many of these failure and incident investigations is the development of an improved understanding of not only the state-of-the-art in experimental and analytical methods but also the state-of-the-art in the design and manufacturing processes that may contribute to such failures. In order to provide insight into such large-scale investigations, a series of lessons learned were captured. Awareness of these lessons learned is highly beneficial to engineers involved in similar investigations. Therefore, it is prudent that the lessons learned are disseminated such that they can be built upon in other investigations and in ensuing research and development activities

Noise analysis for 3-point chemical shift-based water-fat separation with spectral modeling of fat

Purpose: To model the theoretical signal-to-noise ratio (SNR) behavior of 3-point chemical shift-based water-fat separation, using spectral modeling of fat, with experimental validation for spin-echo and gradient-echo imaging. The echo combination that achieves the best SNR performance for a given spectral model of fat was also investigated. Materials and Methods: Cramér-Rao bound analysis was used to calculate the best possible SNR performance for a given echo combination. Experimental validation in a fatwater phantom was performed and compared with theory. In vivo scans were performed to compare fat separation with and with out spectral modeling of fat. Results: Theoretical SNR calculations for methods that include spectral modeling of fat agree closely with experimental SNR measurements. Spectral modeling of fat more accurately separates fat and water signals, with only a slight decrease in the SNR performance of the water-only image, although with a relatively large decrease in the fat SNR performance. Conclusion: The optimal echo combination that provides the best SNR performance for water using spectral modeling of fat is very similar to previous optimizations that modeled fat as a single peak. Therefore, the optimal echo spacing commonly used for single fat peak models is adequate for most applications that use spectral modeling of fat. © 2010 Wiley-Liss, Inc

Phase and amplitude correction for multi-echo water-fat separation with bipolar acquisitions

Purpose: To address phase and amplitude errors for multi-point water-fat separation with bipolar acquisitions, which efficiently collect all echoes with alternating read-out gradient polarities in one repetition. Materials and Methods: With the bipolar acquisitions, eddy currents and other system nonidealities can induce inconsistent phase errors between echoes, disrupting water-fat separation. Previous studies have addressed phase correction in the read-out direction. However, the bipolar acquisitions may be subject to spatially high order phase errors as well as an amplitude modulation in the read-out direction. A method to correct for the 2D phase and amplitude errors is introduced. Low resolution reference data with reversed gradient polarities are collected. From the pair of low-resolution data collected with opposite gradient polarities, the two-dimensional phase errors are estimated and corrected. The pair of data are then combined for water-fat separation. Results: We demonstrate that the proposed method can effectively remove the high order errors with phantom and in vivo experiments, including obliquely oriented scans. Conclusion: For bipolar multi-echo acquisitions, uniform water-fat separation can be achieved by removing high order phase errors with the proposed method. © 2010 Wiley-Liss, Inc

The decline of water hyacinth on Lake Victoria was due to biological control by Neochetina spp

There has been some debate recently about the cause of the decline of water hyacinth on Lake Victoria. While much of this evidence points to classical biological control as the major factor, the El Niño associated weather pattern of the last quarter of 1997 and the first half of 1998 has confused the issue. We argue first that the reductions in water hyacinth on Lake Victoria were ultimately caused by the widespread and significant damage to plants by Neochetina spp., although this process was increased by the stormy weather associated with the El Niño event; second that increased waves and current on Lake Victoria caused by El Niño redistributed water hyacinth plants around the lake; and third that a major lake-wide resurgence of water hyacinth plants on Lake Victoria has not occurred and will not occur unless the weevil populations are disrupted. We conclude that the population crash of water hyacinth on Lake Victoria would not have occurred in the absence of the weevils, but that it may have been hastened by stormy weather associated with the El Niño event