78 research outputs found
1937: Abilene Christian College Bible Lectures - Full Text
Delivered in the auditorium of Abilene Christian College, Abilene, Texas, February 193
Actin- and Dynamin-Dependent Maturation of Bulk Endocytosis Restores Neurotransmission following Synaptic Depletion
Bulk endocytosis contributes to the maintenance of neurotransmission at the amphibian neuromuscular junction by regenerating synaptic vesicles. How nerve terminals internalize adequate portions of the presynaptic membrane when bulk endocytosis is initiated before the end of a sustained stimulation is unknown. A maturation process, occurring at the end of the stimulation, is hypothesised to precisely restore the pools of synaptic vesicles. Using confocal time-lapse microscopy of FM1-43-labeled nerve terminals at the amphibian neuromuscular junction, we confirm that bulk endocytosis is initiated during a sustained tetanic stimulation and reveal that shortly after the end of the stimulation, nerve terminals undergo a maturation process. This includes a transient bulging of the plasma membrane, followed by the development of large intraterminal FM1-43-positive donut-like structures comprising large bulk membrane cisternae surrounded by recycling vesicles. The degree of bulging increased with stimulation frequency and the plasmalemma surface retrieved following the transient bulging correlated with the surface membrane internalized in bulk cisternae and recycling vesicles. Dyngo-4a, a potent dynamin inhibitor, did not block the initiation, but prevented the maturation of bulk endocytosis. In contrast, cytochalasin D, an inhibitor of actin polymerization, hindered both the initiation and maturation processes. Both inhibitors hampered the functional recovery of neurotransmission after synaptic depletion. Our data confirm that initiation of bulk endocytosis occurs during stimulation and demonstrates that a delayed maturation process controlled by actin and dynamin underpins the coupling between exocytosis and bulk endocytosis
Gas source isotope ratio mass spectrometry (IRMS)
Gas source isotope ratio mass spectrometry is usually referred to as isotope ratio mass spectrometry (IRMS) or stable-isotope ratio mass spectrometry (SIRMS). IRMS is a conventional method for measuring isotope ratios and has benefited from more than 65 years of research and development. Modern mass spectrometers are all based on gas source isotope ratio mass spectrometry field mass separators. More recently, the development of high-resolution sector field devices has added a new dimension to IRMS. Modern instruments achieve a high sample throughput, which is a prerequisite, e.g., for ecosystem studies where usually a large number of samples needs to be analysed and high precision is required. IRMS is used specifically for the measurement of stable-isotope ratios of a limited number of elements (C, H, N, O and S) after transfer into a gaseous species. Si, Cl, Br and Se can be added to the list even though their applications are limited compared to the other isotope systems. A concise overview of the technical background is given here as well as numerous applications of this technique in earth and geosciences, paleoclimate research, cosmochemistry, environmental sciences and life sciences
Entry-Level Competencies and Attributes Needed by Interior Design Graduates: A Survey of Top Interior Design Firms
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