2,614 research outputs found
Neural locus of color afterimages.
After fixating on a colored pattern, observers see a similar pattern in complementary colors when the stimulus is removed. Afterimages were important in disproving the theory that visual rays emanate from the eye[1], in demonstrating inter-ocular interactions[2], and in revealing the independence of binocular-vision from eye-movements[3]. Afterimages also prove invaluable in exploring selective attention[4], filling-in[5], and consciousness[6]. Proposed physiological mechanisms for color afterimages range from bleaching of cone photo-pigments[7] to cortical adaptation[4–6, 8, 9], but direct neural measurements have not been reported. We introduce a time-varying method for evoking after-images, which provides precise measurements of adaptation and a direct link between visual percepts and neural responses[10]. We then use in vivo electrophysiological recordings to show that all three classes of primate retinal ganglion cells exhibit subtractive adaptation to prolonged stimuli, with much slower time-constants than those expected of photoreceptors. At the cessation of the stimulus, ganglion cells generate rebound responses that can provide afterimage signals for later neurons. Our results indicate that afterimage signals are generated in the retina, but may be modified like other retinal signals by cortical processes[4–6], so that evidence presented for cortical generation of color afterimages[8, 9] is explainable by spatio-temporal factors that apply to all signals
The peritoneal tumour microenvironment of high-grade serous ovarian cancer
High-grade serous ovarian cancer (HGSC) disseminates early and extensively throughout the peritoneal space, causing multiple lesions that are a major clinical problem. The aim of this study was to investigate the cellular composition of peritoneal tumour deposits in patient biopsies and their evolution in mouse models using immunohistochemistry, intravital microscopy, confocal microscopy, and 3D modelling. Tumour deposits from the omentum of HGSC patients contained a prominent leukocyte infiltrate of CD3(+) T cells and CD68(+) macrophages, with occasional neutrophils. Alpha-smooth muscle actin(+) (α-SMA(+) ) pericytes and/or fibroblasts surrounded these well-vascularized tumour deposits. Using the murine bowel mesentery as an accessible mouse peritoneal tissue that could be easily imaged, and two different transplantable models, we found multiple microscopic tumour deposits after i.p. injection of malignant cells. Attachment to the peritoneal surface was rapid (6-48 h) with an extensive CD45(+) leukocyte infiltrate visible by 48 h. This infiltrate persisted until end point and in the syngeneic murine ID8 model, it primarily consisted of CD3(+) T lymphocytes and CD68(+) macrophages with α-SMA(+) cells also involved from the earliest stages. A majority of tumour deposits developed above existing mesenteric blood vessels, but in avascular spaces new blood vessels tracked towards the tumour deposits by 2-3 weeks in the IGROV-1 xenografts and 6 weeks in the ID8 syngeneic model; a vigorous convoluted blood supply was established by end point. Inhibition of tumour cell cytokine production by stable expression of shRNA to CXCR4 in IGROV-1 cells did not influence the attachment of cells to the mesentery but delayed neovascularization and reduced tumour deposit size. We conclude that the multiple peritoneal tumour deposits found in HGSC patients can be modelled in the mouse. The techniques described here may be useful for assessing treatments that target the disseminated stage of this disease
MYSTIC: Michigan Young STar Imager at CHARA
We present the design for MYSTIC, the Michigan Young STar Imager at CHARA.
MYSTIC will be a K-band, cryogenic, 6-beam combiner for the Georgia State
University CHARA telescope array. The design follows the image-plane
combination scheme of the MIRC instrument where single-mode fibers bring
starlight into a non-redundant fringe pattern to feed a spectrograph. Beams
will be injected in polarization-maintaining fibers outside the cryogenic dewar
and then be transported through a vacuum feedthrough into the ~220K cold volume
where combination is achieved and the light is dispersed. We will use a C-RED
One camera (First Light Imaging) based on the eAPD SAPHIRA detector to allow
for near-photon-counting performance. We also intend to support a 4-telescope
mode using a leftover integrated optics component designed for the VLTI-GRAVITY
experiment, allowing better sensitivity for the faintest targets. Our primary
science driver motivation is to image disks around young stars in order to
better understand planet formation and how forming planets might influence disk
structures.Comment: Presented at the 2018 SPIE Astronomical Telescopes + Instrumentation,
Austin, Texas, US
The Behavior of Granular Materials under Cyclic Shear
The design and development of a parallel plate shear cell for the study of
large scale shear flows in granular materials is presented. The parallel plate
geometry allows for shear studies without the effects of curvature found in the
more common Couette experiments. A system of independently movable slats
creates a well with side walls that deform in response to the motions of grains
within the pack. This allows for true parallel plate shear with minimal
interference from the containing geometry. The motions of the side walls also
allow for a direct measurement of the velocity profile across the granular
pack. Results are presented for applying this system to the study of transients
in granular shear and for shear-induced crystallization. Initial shear profiles
are found to vary from packing to packing, ranging from a linear profile across
the entire system to an exponential decay with a width of approximately 6 bead
diameters. As the system is sheared, the velocity profile becomes much sharper,
resembling an exponential decay with a width of roughly 3 bead diameters.
Further shearing produces velocity profiles which can no longer be fit to an
exponential decay, but are better represented as a Gaussian decay or error
function profile. Cyclic shear is found to produce large scale ordering of the
granular pack, which has a profound impact on the shear profile. There exist
periods of time in which there is slipping between layers as well as periods of
time in which the layered particles lock together resulting in very little
relative motion.Comment: 10 pages including 12 figure
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Threats to the validity of the Collegiate Learning Assessment (CLA+) as a measure of critical thinking skills and implications for Learning Gain
The University of Reading Learning Gain project is a three-year longitudinal project to test and evaluate a range of available methodologies and to draw conclusions on what might be the right combination of instruments for the measurement of Learning Gain in higher education. This paper analyses the validity of a measure of critical thinking skills, the Collegiate Learning Assessment (CLA+) and the implications of using this standardised test as a proxy for Learning Gain. The paper reviews five inferences regarding the interpretations and use of test scores: construct representation, scoring, generalisation, extrapolation and decision-making. Each section reviews some of the available evidence in support of the claims the CLA+ makes and the threats to their validity. The possible impact of these issues on Learning Gain in the UK is considered
Experienced tutors' deployment of thinking skills and what might be entailed in enhancing such skills
In the context of research that reports weaknesses in adults' critical thinking skills, the primary aim was to examine adults' use of critical thinking skills that are described in taxonomies and to identify areas for development. Position papers written by an opportunity sample of 32 experienced adult educators formed the data for a descriptive sample survey design intended to reveal participants' use of critical thinking skills. Each 6000-word paper was written during a development programme that supported such skills. A content analysis of the papers revealed that when participants drew on personal and published ideas about learning to derive their proposals for change, they accepted the ideas uncritically, thereby implying that they might find it difficult to help learners to examine ideas critically. The evidence supports research that implies that critical thinking skills are unlikely to develop unless overall course design privileges the development of epistemological understanding (King and Kitchener 1994, Kuhn 1999). A fundamental assumption underlying the study is that this understanding influences effective citizenship and personal development, as well as employability. A proposition that merits attention in future research is that the development of epistemological understanding is largely neglected in current curricula in formal post-16 education
Dirac R-matrix calculations for the electron-impact excitation of neutral tungsten providing noninvasive diagnostics for magnetic confinement fusion
Neutral tungsten is the primary candidate as a wall material in the divertor
region of the International Thermonuclear Experimental Reactor (ITER). The
efficient operation of ITER depends heavily on precise atomic physics
calculations for the determination of reliable erosion diagnostics, helping to
characterise the influx of tungsten impurities into the core plasma. The
following paper presents detailed calculations of the atomic structure of
neutral tungsten using the multiconfigurational Dirac-Fock method, drawing
comparisons with experimental measurements where available, and includes a
critical assessment of existing atomic structure data. We investigate the
electron-impact excitation of neutral tungsten using the Dirac R-matrix method
and, by employing collisional-radiative models, we benchmark our results with
recent Compact Toroidal Hybrid measurements. The resulting comparisons
highlight alternative diagnostic lines to the widely used 400.88nm line.Comment: 10 pages, 6 figure
Coherence Imaging Spectroscopy systems on Wendelstein 7-X for studies of island divertor plasma behavior
Freshly Formed Dust in the Cassiopeia A Supernova Remnant as Revealed by the Spitzer Space Telescope
We performed Spitzer Infrared Spectrograph mapping observations covering
nearly the entire extent of the Cassiopeia A supernova remnant (SNR), producing
mid-infrared (5.5-35 micron) spectra every 5-10". Gas lines of Ar, Ne, O, Si, S
and Fe, and dust continua were strong for most positions. We identify three
distinct ejecta dust populations based on their continuum shapes. The dominant
dust continuum shape exhibits a strong peak at 21 micron. A line-free map of 21
micron-peak dust made from the 19-23 micron range closely resembles the [Ar
II], [O IV], and [Ne II] ejecta-line maps implying that dust is freshly formed
in the ejecta. Spectral fitting implies the presence of SiO2, Mg
protosilicates, and FeO grains in these regions. The second dust type exhibits
a rising continuum up to 21 micron and then flattens thereafter. This ``weak 21
micron'' dust is likely composed of Al2O3 and C grains. The third dust
continuum shape is featureless with a gently rising spectrum and is likely
composed of MgSiO3 and either Al2O3 or Fe grains. Using the least massive
composition for each of the three dust classes yields a total mass of 0.02
Msun. Using the most-massive composition yields a total mass of 0.054 Msun. The
primary uncertainty in the total dust mass stems from the selection of the dust
composition necessary for fitting the featureless dust as well as 70 micron
flux. The freshly formed dust mass derived from Cas A is sufficient from SNe to
explain the lower limit on the dust masses in high redshift galaxies.Comment: 8 figures: Accepted for the publication in Ap
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