Quantification of gliadin levels to the picogram level by flow cytometry

Celiac disease is a widely prevalent enteropathy caused by intolerance to gliadin, one of the gluten proteins. We developed two methods for the analysis of gliadin levels. Both methods use flow cytometry and rat antibodies against a 16-residue peptide of gliadin. The peptide is common to the alpha-, beta-, gamma-, and omega-gliadins

The World Formula One Championship and North America: The Development of a Globalized Industrial Sports Complex?

This research seeks to recognize the World Formula One Championship as a global industrial sports complex through both its direct and indirect connections with North America. The study analyses the visibly linked and imperceptible separate commercial, cultural, political, and technological inputs from data retrieved from several accepted disciplines that can be prejudiced based on the sport’s respective nationalism. The study examines the entangled but corresponding economic and social engines that constitute the global framework of the World Formula One Championship. It does this by examining those commercial, cultural, political, and technological dimensions that form this autonomous industrial sports complex. This thesis presents research that scrutinizes how the World Formula One Championship cannot be understood as strictly a European enterprise and discovers intricate global relationships, particularly North American contributions, which are often disregarded, despite its historical existence on the continent and the more recent presence of the sport in the United States. These connections include a vast array of shared relationships that reveals how the World Formula One Championship is a globalized and evolving industrial sports complex of its own significance. Competition from other major league sports, lack of media coverage, and a small, isolated audience has caused the World Formula One Championship to be misunderstood in much of North America. The verified associations that materialize in this study demonstrate its importance and influence as an all-encompassing business and worldwide sport, in fact, one of three Mega Sport

Microbiota–Liver Diseases Interactions

: Gut microbiota regulates essential processes of host metabolism and physiology: synthesis of vitamins, digestion of foods non-digestible by the host (such as fibers), and-most important-protects the digestive tract from pathogens. In this study, we focus on the CRISPR/Cas9 technology, which is extensively used to correct multiple diseases, including liver diseases. Then, we discuss the non-alcoholic fatty liver disease (NAFLD), affecting more than 25% of the global population; colorectal cancer (CRC) is second in mortality. We give space to rarely discussed topics, such as pathobionts and multiple mutations. Pathobionts help to understand the origin and complexity of the microbiota. Since several types of cancers have as target the gut, it is vital extending the research of multiple mutations to the type of cancers affecting the gut-liver axis

Antimicrobial peptides from plants: stabilization of the core of a tomato defensin by intramolecular disulfide bond

Cysteine-containing antimicrobial peptides of diverse phylogeny share a common structural signature, the γ core, characterized by a strong polarization of charges in two antiparallel β sheets. In this work, we analyzed peptides derived from the tomato defensin SolyC07g007760 corresponding to the protein γ core and demonstrated that cyclization of the peptides, which results in segregation of positive charges to the turn region, produces peptides very active against Gram negative bacteria, such as Salmonella enterica and Helicobacter pylori. Interestingly, these peptides show very low hemolytic activity and thus represent a scaffold for the design of new antimicrobial peptides

Big data managing in a landslide early warning system: Experience from a ground-based interferometric radar application

A big challenge in terms or landslide risk mitigation is represented by increasing the resiliency of society exposed to the risk. Among the possible strategies with which to reach this goal, there is the implementation of early warning systems. This paper describes a procedure to improve early warning activities in areas affected by high landslide risk, such as those classified as critical infrastructures for their central role in society. This research is part of the project LEWIS (Landslides Early Warning Integrated System): An Integrated System for Landslide Monitoring, Early Warning and Risk Mitigation along Lifelines. LEWIS is composed of a susceptibility assessment methodology providing information for single points and areal monitoring systems, a data transmission network and a data collecting and processing center (DCPC), where readings from all monitoring systems and mathematical models converge and which sets the basis for warning and intervention activities. The aim of this paper is to show how logistic issues linked to advanced monitoring techniques, such as big data transfer and storing, can be dealt with compatibly with an early warning system. Therefore, we focus on the interaction between an areal monitoring tool (a ground-based interferometric radar) and the DCPC. By converting complex data into ASCII strings and through appropriate data cropping and average, and by implementing an algorithm for line-of-sight correction, we managed to reduce the data daily output without compromising the capability for performing

Barrier and mechanical properties of clay-reinforced polymeric nanocomposites

In this work, clay-based nanocomposites films were prepared by addition of clay-Na+ natural montmorillonite in pectin and hydroxypropyl methylcellulose (HPMC) matrices. Mechanical (tensile strength, elastic modulus, and elongation) and barrier (Water Vapor Permeability (WVP), and Oxygen permeability (O2P)) properties were investigated. From results, it was observed that the WVP and O2P decreased when nanoclay was included into the HPMC and pectin matrix films. Additionally, the incorporation of nanoclay in the films significantly improved the mechanical properties because the reinforcing effect of clay from its high aspect ratio and its enormous surface area. These results are very important in packaging area.CNPqFINEPFAPES