Regulation of Acetylated Low Density Lipoprotein Uptake in Macrophages by Pertussis Toxin-Sensitive G Proteins

Class A scavenger receptors (SR-A) mediate the uptake of modified low density lipoprotein (LDL) by macrophages. Although not typically associated with the activation of intracellular signaling cascades, results with peritoneal macrophages indicate that the SR-A ligand acetylated LDL (AcLDL) promotes activation of cytosolic kinases and phospholipases. These signaling responses were blocked by the treatment of cells with pertussis toxin (PTX) indicating that SR-A activates Gi/o-linked signaling pathways. The functional significance of SR-A-mediated Gi/o activation is not clear. In this study, we investigated the potential role of Gi/o activation in regulating SR-A-mediated lipoprotein uptake. Treatment of mouse peritoneal macrophages with PTX decreased association of fluorescently labeled AcLDL with cells. This inhibition was dependent on the catalytic activity of the toxin confirming that the decrease in AcLDL uptake involved inhibiting Gi/o activation. In contrast to the inhibitory effect on AcLDL uptake, PTX treatment did not alter β-VLDL-induced cholesterol esterification or deposition of cholesterol. The ability of polyinosine to completely inhibit AcLDL uptake, and the lack of PTX effect on β-VLDL uptake, demonstrated that the inhibitory effect is specific for SR-A and not the result of non-specific effects on lipoprotein metabolism. Despite having an effect on an SR-A-mediated lipoprotein uptake, there was no change in the relative abundance of SR-A protein after PTX treatment. These results demonstrate that activation of a PTX-sensitive G protein is involved in a feedback process that positively regulates SR-A function

Initial Results from a Stacked Ring Apparatus for Simulation of a Soil Profile

A stack of 48 rings, lined with a latex membrane, is used to confine a column of soil 12 inches high by 12 inches in diameter (300 mm x 300 mm). Both dry and saturated columns of fine sand are shaken at their base, at a centrifugal acceleration of 35.5 g. Measurements of the settlement of the surface, horizontal displacement and pore pressures show that the columns of soil are behaving essentially (although not exactly) as one-dimensional shear beams

A Richness Study of 14 Distant X-ray Clusters From the 160 Square Degree Survey

We have measured the surface density of galaxies toward 14 X-ray-selected cluster candidates at redshifts greater than z=0.46, and we show that they are associated with rich galaxy concentrations. We find that the clusters range between Abell richness classes 0-2, and have a most probable richness class of one. We compare the richness distribution of our distant clusters to those for three samples of nearby clusters with similar X-ray luminosities. We find that the nearby and distant samples have similar richness distributions, which shows that clusters have apparently not evolved substantially in richness since redshift z =0.5. We compare the distribution of distant X-ray clusters in the L_x--richness plane to the distribution of optically-selected clusters from the Palomar Distant Cluster Survey. The optically-selected clusters appear overly rich for their X-ray luminosities when compared to X-ray-selected clusters. Apparently, X-ray and optical surveys do not necessarily sample identical mass concentrations at large redshifts. This may indicate the existence of a population of optically rich clusters with anomalously low X-ray emission. More likely, however, it reflects the tendency for optical surveys to select unvirialized mass concentrations, as might be expected when peering along large-scale filaments.Comment: The abstract has been abridged. Accepted for publication in the Astrophysical Journa

Atomic Scale Memory at a Silicon Surface

The limits of pushing storage density to the atomic scale are explored with a memory that stores a bit by the presence or absence of one silicon atom. These atoms are positioned at lattice sites along self-assembled tracks with a pitch of 5 atom rows. The writing process involves removal of Si atoms with the tip of a scanning tunneling microscope. The memory can be reformatted by controlled deposition of silicon. The constraints on speed and reliability are compared with data storage in magnetic hard disks and DNA.Comment: 13 pages, 5 figures, accepted by Nanotechnolog

Speech Communication

Contains research objectives and three research projects.U. S. Air Force (Electronic Systems Division) under Contract AF 19(604)-6102National Science Foundation (Grant G-16526)National Institutes of Health (Grant MH-04737-02

Asymptotic Structure of Symmetry Reduced General Relativity

Gravitational waves with a space-translation Killing field are considered. In this case, the 4-dimensional Einstein vacuum equations are equivalent to the 3-dimensional Einstein equations with certain matter sources. This interplay between 4- and 3- dimensional general relativity can be exploited effectively to analyze issues pertaining to 4 dimensions in terms of the 3-dimensional structures. An example is provided by the asymptotic structure at null infinity: While these space-times fail to be asymptotically flat in 4 dimensions, they can admit a regular completion at null infinity in 3 dimensions. This completion is used to analyze the asymptotic symmetries, introduce the analog of the 4-dimensional Bondi energy-momentum and write down a flux formula. The analysis is also of interest from a purely 3-dimensional perspective because it pertains to a diffeomorphism invariant 3-dimensional field theory with {\it local} degrees of freedom, i.e., to a midi-superspace. Furthermore, due to certain peculiarities of 3 dimensions, the description of null infinity does have a number of features that are quite surprising because they do not arise in the Bondi-Penrose description in 4 dimensions.Comment: 39 Pages, REVTEX, CGPG-96/5-

Characterization of the JWST Pathfinder Mirror Dynamics Using the Center of Curvature Optical Assembly (CoCOA)

The JWST (James Webb Space Telescope) Optical Telescope Element (OTE) consists of a 6.6 meter clear aperture, 18-segment primary mirror, all-reflective, three-mirror anastigmat operating at cryogenic temperatures. To verify performance of the primary mirror, a full aperture center of curvature optical null test is performed under cryogenic conditions in Chamber A at NASA Johnson Space Center using an instantaneous phase measuring interferometer. After phasing the mirrors during the JWST Pathfinder testing, the interferometer is utilized to characterize the mirror relative piston and tilt dynamics under different facility configurations. The correlation between the motions seen on detectors at the focal plane and the interferometer validates the use of the interferometer for dynamic investigations. The success of planned test hardware improvements will be characterized by the multi-wavelength interferometer (MWIF) at the Center of Curvature Optical Assembly (CoCOA)

Static charged perfect fluid spheres in general relativity

Interior perfect fluid solutions for the Reissner-Nordstrom metric are studied on the basis of a new classification scheme. General formulas are found in many cases. Explicit new global solutions are given as illustrations. Known solutions are briefly reviewed.Comment: 23 pages, Revtex (galley), journal version, to appear in Phys.Rev.