Ras-mediated phosphorylation of a conserved threonine residue enhances the transactivation activities of c-Ets1 and c-Ets2

The Ras oncogene products regulate the expression of genes in transformed cells, and members of the Ets family of transcription factors have been implicated in this process. To determine which Ets factors are the targets of Ras signaling pathways, the abilities of several Ets factors to activate Ras-responsive enhancer (RRE) reporters in the presence of oncogenic Ras were examined. In transient transfection assay, reporters containing RREs composed of Ets-AP-1 binding sites could be activated 30-fold in NIH 3T3 fibroblasts and 80-fold in the macrophage-like line RAW264 by the combination of Ets1 or Ets2 and Ras but not by several other Ets factors that were tested in the assay. Ets2 and Ras also superactivated an RRE composed of Ets-Ets binding sites, but the Ets-responsive promoter of the c-fms gene was not superactivated. Mutation of a threonine residue to alanine in the conserved amino-terminal regions of Ets1 and Ets2 (threonine 38 and threonine 72, respectively) abrogated the ability of each of these proteins to superactivate reporter gene expression. Phosphoamino acid analysis of radiolabeled Ets2 revealed that Ras induced normally absent threonine-specific phosphorylation of the protein. The Ras-dependent increase in threonine phosphorylation was not observed in Ets2 proteins that had the conserved threonine 72 residue mutated to alanine or serine. These data indicate that Ets1 and Ets2 are specific nuclear targets of Ras signaling events and that phosphorylation of a conserved threonine residue is a necessary molecular component of Ras-mediated activation of these transcription factors

X-ray Emission Properties of Large Scale Jets, Hotspots and Lobes in Active Galactic Nuclei

We examine a systematic comparison of jet-knots, hotspots and radio lobes recently observed with Chandra and ASCA. This report will discuss the origin of their X-ray emissions and investigate the dynamics of the jets. The data was compiled at well sampled radio (5GHz) and X-ray frequencies (1keV) for more than 40 radio galaxies. We examined three models for the X-ray production: synchrotron (SYN), synchrotron self-Compton (SSC) and external Compton on CMB photons (EC). For the SYN sources -- mostly jet-knots in nearby low-luminosity radio galaxies -- X-ray photons are produced by ultrarelativistic electrons with energies 10-100 TeV that must be accelerated in situ. For the other objects, conservatively classified as SSC or EC sources, a simple formulation of calculating the ``expected'' X-ray fluxes under an equipartition hypothesis is presented. We confirmed that the observed X-ray fluxes are close to the expected ones for non-relativistic emitting plasma velocities in the case of radio lobes and majority of hotspots, whereas considerable fraction of jet-knots is too bright at X-rays to be explained in this way. We examined two possibilities to account for the discrepancy in a framework of the inverse-Compton model: (1) magnetic field is much smaller than the equipartition value, and (2) the jets are highly relativistic on kpc/Mpc scales. We concluded, that if the inverse-Compton model is the case, the X-ray bright jet-knots are most likely far from the minimum-power condition. We also briefly discuss the other possibility, namely that the observed X-ray emission from all of the jet-knots is synchrotron in origin.Comment: 20 pages, 10 figures, accepted for publication in the Astrophysical Journal, vol.62

Cosmic ray diffusive acceleration at shock waves with finite upstream and downstream escape boundaries

In the present paper we discuss the modifications introduced into the first-order Fermi shock acceleration process due to a finite extent of diffusive regions near the shock or due to boundary conditions leading to an increased particle escape upstream and/or downstream the shock. In the considered simple example of the planar shock wave we idealize the escape phenomenon by imposing a particle escape boundary at some distance from the shock. Presence of such a boundary (or boundaries) leads to coupled steepening of the accelerated particle spectrum and decreasing of the acceleration time scale. It allows for a semi-quantitative evaluation and, in some specific cases, also for modelling of the observed steep particle spectra as a result of the first-order Fermi shock acceleration. We also note that the particles close to the upper energy cut-off are younger than the estimate based on the respective acceleration time scale. In Appendix A we present a new time-dependent solution for infinite diffusive regions near the shock allowing for different constant diffusion coefficients upstream and downstream the shock.Comment: LaTeX, 14 pages, 4 postscript figures; Solar Physics (accepted

Multi-Organizational Strategies: An Analytic Framework and Case Illustrations

Last November the Management and Technology Area had its Status Report on the results of its research activities during 1976. This Research Memorandum is the report that the internal "Organizations Group" of Management and Technology presented. Two different case studies are reported. The first one is concerned with the impacts of oil development off-shore of the North East coast of Scotland. While presenting some of the findings specific to Scotland, our main intention with this part of the paper is to convey the methodology we have developed to study the organizational dimension of large development programs. This methodology is used in the second part, the Bratsk-Ilimsk Territorial Production Complex case study, to do the analysis of a particular policy issue today under the attention of Soviet policy makers, namely the need for new management mechanisms to support the present evolution of territorial production complexes

The Electron Energy Distribution in the Hotspots of Cygnus A: Filling the Gap with the Spitzer Space Telescope

Here we present Spitzer Space Telescope imaging of Cyg A with the Infrared Array Camera, resulting in the detection of the high-energy tails or cut-offs in the synchrotron spectra for all four hotspots of this archetype radio galaxy. When combined with the other data collected from the literature, our observations allow for detailed modeling of the broad-band emission for the brightest spots A and D. We confirm that the X-ray flux detected previously from these features is consistent with the synchrotron self-Compton radiation for the magnetic field intensity 170 muG in spot A, and 270 muG in spot D. We also find that the energy density of the emitting electrons is most likely larger by a factor of a few than the energy density of the hotspots' magnetic field. We construct energy spectra of the radiating ultrarelativistic electrons. We find that for both hotspots A and D these spectra are consistent with a broken power-law extending from at least 100 MeV up to 100 GeV, and that the spectral break corresponds almost exactly to the proton rest energy of 1 GeV. We argue that the shape of the electron continuum reflects two different regimes of the electron acceleration process at mildly relativistic shocks, rather than resulting from radiative cooling and/or absorption effects. In this picture the protons' inertia defines the critical energy for the hotspot electrons above which Fermi-type acceleration processes may play a major role, but below which the operating acceleration mechanism has to be of a different type. At energies >100 GeV, the electron spectra cut-off/steepen again, most likely as a result of spectral aging due to radiative loss effects. We discuss several implications of the presented analysis for the physics of extragalactic jets.Comment: 29 pages, 8 figures and 2 tables included. Accepted for publication in Ap

High Energy gamma-rays From FR I Jets

Thanks to Hubble and Chandra telescopes, some of the large scale jets in extragalactic radio sources are now being observed at optical and X-ray frequencies. For the FR I objects the synchrotron nature of this emission is surely established, although a lot of uncertainties - connected for example with the particle acceleration processes involved - remain. In this paper we study production of high energy gamma-rays in FR I kiloparsec-scale jets by inverse-Compton emission of the synchrotron-emitting electrons. We consider different origin of seed photons contributing to the inverse-Compton scattering, including nuclear jet radiation as well as ambient, stellar and circumstellar emission of the host galaxies. We discuss how future detections or non-detections of the evaluated gamma-ray fluxes can provide constraints on the unknown large scale jet parameters, i.e. the magnetic field intensity and the jet Doppler factor. For the nearby sources Centaurus A and M 87, we find measurable fluxes of TeV photons resulting from synchrotron self-Compton process and from comptonisation of the galactic photon fields, respectively. In the case of Centaurus A, we also find a relatively strong emission component due to comptonisation of the nuclear blazar photons, which could be easily observed by GLAST at energy ~10 GeV, providing important test for the unification of FR I sources with BL Lac objects.Comment: 39 pages, 6 figures included. Modified version, accepted for publication in Astrophysical Journa

Spectroscopic Survey of {\gamma} Doradus Stars I. Comprehensive atmospheric parameters and abundance analysis of {\gamma} Doradus stars

We present a spectroscopic survey of known and candidate $\gamma$\,Doradus stars. The high-resolution, high signal-to-noise spectra of 52 objects were collected by five different spectrographs. The spectral classification, atmospheric parameters (\teff, $\log g$, $\xi$), $v\sin i$ and chemical composition of the stars were derived. The stellar spectral and luminosity classes were found between G0-A7 and IV-V, respectively. The initial values for \teff\ and \logg\ were determined from the photometric indices and spectral energy distribution. Those parameters were improved by the analysis of hydrogen lines. The final values of \teff, \logg\ and $\xi$ were derived from the iron lines analysis. The \teff\ values were found between 6000\,K and 7900\,K, while \logg\,values range from 3.8 to 4.5\,dex. Chemical abundances and $v\sin i$ values were derived by the spectrum synthesis method. The $v\sin i$ values were found between 5 and 240\,km\,s$^{-1}$. The chemical abundance pattern of $\gamma$\,Doradus stars were compared with the pattern of non-pulsating stars. It turned out that there is no significant difference in abundance patterns between these two groups. Additionally, the relations between the atmospheric parameters and the pulsation quantities were checked. A strong correlation between the $v\sin i$ and the pulsation periods of $\gamma$\,Doradus variables was obtained. The accurate positions of the analysed stars in the H-R diagram have been shown. Most of our objects are located inside or close to the blue edge of the theoretical instability strip of $\gamma$\,Doradus.Comment: 18 pages, 13 figure

First presentation of LPIN1 acute rhabdomyolysis in adolescence and adulthood

LPIN1 mutations are a known common cause of autosomal recessive, recurrent and life-threatening acute rhabdomyolysis of childhood-onset. The first episode of rhabdomyolysis usually happens in nearly all cases before the age of 5 and death is observed in 1/3 of patients. Here we present two cases of acute rhabdomyolysis with a milder phenotype caused by LPIN1 mutation presenting in adolescence (11 years old) and adulthood (40 years old) after Parvovirus infection and metabolic stress, respectively. In our opinion, the mutation types, epigenetic factors, the environment exposition to triggers or the existence of proteins with a similar structure of LPIN1, may have a role in modulating the onset of rhabdomyolysis. LPIN1 should be included on a panel of genes analysed in the investigation of adult individuals with rhabdomyolysis. Metabolic and viral stressors should be included in the list of possible rhabdomyolysis precipitant

Particle Diffusion and Acceleration by Shock Wave in Magnetized Filamentary Turbulence

We expand the off-resonant scattering theory for particle diffusion in magnetized current filaments that can be typically compared to astrophysical jets, including active galactic nucleus jets. In a high plasma beta region where the directional bulk flow is a free-energy source for establishing turbulent magnetic fields via current filamentation instabilities, a novel version of quasi-linear theory to describe the diffusion of test particles is proposed. The theory relies on the proviso that the injected energetic particles are not trapped in the small-scale structure of magnetic fields wrapping around and permeating a filament but deflected by the filaments, to open a new regime of the energy hierarchy mediated by a transition compared to the particle injection. The diffusion coefficient derived from a quasi-linear type equation is applied to estimating the timescale for the stochastic acceleration of particles by the shock wave propagating through the jet. The generic scalings of the achievable highest energy of an accelerated ion and electron, as well as of the characteristic time for conceivable energy restrictions, are systematically presented. We also discuss a feasible method of verifying the theoretical predictions. The strong, anisotropic turbulence reflecting cosmic filaments might be the key to the problem of the acceleration mechanism of the highest energy cosmic rays exceeding 100 EeV (10^{20} eV), detected in recent air shower experiments.Comment: 39 pages, 2 figures, accepted for publication in Ap