Epidemiological features of human brucellosis in central Iran, 2006-2011

Objectives: Brucellosis is still one of the most challenging issues for health and the economy in many developing countries such as Iran. This study investigated the epidemiological features of brucellosis in Isfahan province in central Iran. Study design: This retrospective descriptive study was planned to determine the epidemiological features of brucellosis in central Iran, as this is one of the most endemic areas in the country. Methods: Data collection was performed using epidemiological questionnaires from the private and public sectors over a 4-year period (2006-2009). Results: In total, 1996 cases of brucellosis were reported. The incidence of brucellosis decreased from 17.1/100,000 in 2006 to 8.2/100,000 in 2009. The male: female ratio was 2.1, and the disease was most common in individuals aged 15-20 years. Sixty-eight percent of cases were from rural areas, and the animal contact rate was 81% in rural cases and 61% in urban cases. Raw milk was the most commonly consumed dairy product, consumed by 37% of cases. Conclusions: Health-related interventions need to empower communities at risk, especially young men and adult women in the western districts of Isfahan province. Public health promotion is needed for control of risk factors in these areas. (C) 2012 The Royal Society for Public Health. Published by Elsevier Ltd. All rights reserved

The Molecular Basis of COVID-19 Pathogenesis, Conventional and Nanomedicine Therapy

In late 2019, a new member of the Coronaviridae family, officially designated as “severe acute respiratory syndrome coronavirus 2” (SARS-CoV-2), emerged and spread rapidly. The Coronavirus Disease-19 (COVID-19) outbreak was accompanied by a high rate of morbidity and mortality worldwide and was declared a pandemic by the World Health Organization in March 2020. Within the Coronaviridae family, SARS-CoV-2 is considered to be the third most highly pathogenic virus that infects humans, following the severe acute respiratory syndrome coronavirus (SARS-CoV) and the Middle East respiratory syndrome coronavirus (MERS-CoV). Four major mechanisms are thought to be involved in COVID-19 pathogenesis, including the activation of the renin-angiotensin system (RAS) signaling pathway, oxidative stress and cell death, cytokine storm, and endothelial dysfunction. Following virus entry and RAS activation, acute respiratory distress syndrome develops with an oxidative/nitrosative burst. The DNA damage induced by oxidative stress activates poly ADP-ribose polymerase-1 (PARP-1), viral macrodomain of non-structural protein 3, poly (ADP-ribose) glycohydrolase (PARG), and transient receptor potential melastatin type 2 (TRPM2) channel in a sequential manner which results in cell apoptosis or necrosis. In this review, blockers of angiotensin II receptor and/or PARP, PARG, and TRPM2, including vitamin D3, trehalose, tannins, flufenamic and mefenamic acid, and losartan, have been investigated for inhibiting RAS activation and quenching oxidative burst. Moreover, the application of organic and inorganic nanoparticles, including liposomes, dendrimers, quantum dots, and iron oxides, as therapeutic agents for SARS-CoV-2 were fully reviewed. In the present review, the clinical manifestations of COVID-19 are explained by focusing on molecular mechanisms. Potential therapeutic targets, including the RAS signaling pathway, PARP, PARG, and TRPM2, are also discussed in depth