144 research outputs found
Observation of Feshbach-like resonances in collisions between ultracold molecules
We observe magnetically tuned collision resonances for ultracold Cs2
molecules stored in a CO2-laser trap. By magnetically levitating the molecules
against gravity, we precisely measure their magnetic moment. We find an avoided
level crossing which allows us to transfer the molecules into another state. In
the new state, two Feshbach-like collision resonances show up as strong
inelastic loss features. We interpret these resonances as being induced by Cs4
bound states near the molecular scattering continuum. The tunability of the
interactions between molecules opens up novel applications such as controlled
chemical reactions and synthesis of ultracold complex molecules
Experimental Evidence for Efimov Quantum States
Three interacting particles form a system which is well known for its complex
physical behavior. A landmark theoretical result in few-body quantum physics is
Efimov's prediction of a universal set of weakly bound trimer states appearing
for three identical bosons with a resonant two-body interaction. Surprisingly,
these states even exist in the absence of a corresponding two-body bound state
and their precise nature is largely independent of the particular type of the
two-body interaction potential. Efimov's scenario has attracted great interest
in many areas of physics; an experimental test however has not been achieved.
We report the observation of an Efimov resonance in an ultracold thermal gas of
cesium atoms. The resonance occurs in the range of large negative two-body
scattering lengths and arises from the coupling of three free atoms to an
Efimov trimer. We observe its signature as a giant three-body recombination
loss when the strength of the two-body interaction is varied near a Feshbach
resonance. This resonance develops into a continuum resonance at non-zero
collision energies, and we observe a shift of the resonance position as a
function of temperature. We also report on a minimum in the recombination loss
for positive scattering lengths, indicating destructive interference of decay
pathways. Our results confirm central theoretical predictions of Efimov physics
and represent a starting point from which to explore the universal properties
of resonantly interacting few-body systems.Comment: 8 pages, 4 figures, Proceedings of ICAP-2006 (Innsbruck
`St\"uckelberg interferometry' with ultracold molecules
We report on the realization of a time-domain `St\"uckelberg interferometer',
which is based on the internal state structure of ultracold Feshbach molecules.
Two subsequent passages through a weak avoided crossing between two different
orbital angular momentum states in combination with a variable hold time lead
to high-contrast population oscillations. This allows for a precise
determination of the energy difference between the two molecular states. We
demonstrate a high degree of control over the interferometer dynamics. The
interferometric scheme provides new possibilities for precision measurements
with ultracold molecules.Comment: 4 pages, 5 figure
Evidence for Efimov quantum states in an ultracold gas of cesium atoms
Systems of three interacting particles are notorious for their complex
physical behavior. A landmark theoretical result in few-body quantum physics is
Efimov's prediction of a universal set of bound trimer states appearing for
three identical bosons with a resonant two-body interaction.
Counterintuitively, these states even exist in the absence of a corresponding
two-body bound state. Since the formulation of Efimov's problem in the context
of nuclear physics 35 years ago, it has attracted great interest in many areas
of physics. However, the observation of Efimov quantum states has remained an
elusive goal. Here we report the observation of an Efimov resonance in an
ultracold gas of cesium atoms. The resonance occurs in the range of large
negative two-body scattering lengths, arising from the coupling of three free
atoms to an Efimov trimer. Experimentally, we observe its signature as a giant
three-body recombination loss when the strength of the two-body interaction is
varied. We also detect a minimum in the recombination loss for positive
scattering lengths, indicating destructive interference of decay pathways. Our
results confirm central theoretical predictions of Efimov physics and represent
a starting point with which to explore the universal properties of resonantly
interacting few-body systems. While Feshbach resonances have provided the key
to control quantum-mechanical interactions on the two-body level, Efimov
resonances connect ultracold matter to the world of few-body quantum phenomena.Comment: 18 pages, 3 figure
Does gamma-aminobutyric acid (GABA) influence the development of chronic inflammation in rheumatoid arthritis?
<p>Abstract</p> <p>Background</p> <p>Recent studies have demonstrated a role for spinal p38 MAP kinase (MAPK) in the development of chronic inflammation and peripheral arthritis and a role for GABA in the inhibition of p38 MAPK mediated effects. Integrating these data suggests that GABA may play a role in downregulating mechanisms that lead to the production of proinflammatory agents such as interleukin-1, interleukin-6, and matrix metalloproteinase 3 – agents implicated in the pathogenesis of rheumatoid arthritis (RA). Genetic studies have also associated RA with members of the p38 MAPK pathway.</p> <p>Hypothesis</p> <p>We propose a hypothesis for an inefficient GABA signaling system that results in unchecked proinflammatory cytokine production via the p38 MAPK pathway. This model also supports the need for increasing research in the integration of immunology and neuroscience.</p
Future therapeutic targets in rheumatoid arthritis?
Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by persistent joint inflammation. Without adequate treatment, patients with RA will develop joint deformity and progressive functional impairment. With the implementation of treat-to-target strategies and availability of biologic therapies, the outcomes for patients with RA have significantly improved. However, the unmet need in the treatment of RA remains high as some patients do not respond sufficiently to the currently available agents, remission is not always achieved and refractory disease is not uncommon. With better understanding of the pathophysiology of RA, new therapeutic approaches are emerging. Apart from more selective Janus kinase inhibition, there is a great interest in the granulocyte macrophage-colony stimulating factor pathway, Bruton's tyrosine kinase pathway, phosphoinositide-3-kinase pathway, neural stimulation and dendritic cell-based therapeutics. In this review, we will discuss the therapeutic potential of these novel approaches
The Transcription Factor AmrZ Utilizes Multiple DNA Binding Modes to Recognize Activator and Repressor Sequences of Pseudomonas aeruginosa Virulence Genes
AmrZ, a member of the Ribbon-Helix-Helix family of DNA binding proteins, functions as both a transcriptional activator and repressor of multiple genes encoding Pseudomonas aeruginosa virulence factors. The expression of these virulence factors leads to chronic and sustained infections associated with worsening prognosis. In this study, we present the X-ray crystal structure of AmrZ in complex with DNA containing the repressor site, amrZ1. Binding of AmrZ to this site leads to auto-repression. AmrZ binds this DNA sequence as a dimer-of-dimers, and makes specific base contacts to two half sites, separated by a five base pair linker region. Analysis of the linker region shows a narrowing of the minor groove, causing significant distortions. AmrZ binding assays utilizing sequences containing variations in this linker region reveals that secondary structure of the DNA, conferred by the sequence of this region, is an important determinant in binding affinity. The results from these experiments allow for the creation of a model where both intrinsic structure of the DNA and specific nucleotide recognition are absolutely necessary for binding of the protein. We also examined AmrZ binding to the algD promoter, which results in activation of the alginate exopolysaccharide biosynthetic operon, and found the protein utilizes different interactions with this site. Finally, we tested the in vivo effects of this differential binding by switching the AmrZ binding site at algD, where it acts as an activator, for a repressor binding sequence and show that differences in binding alone do not affect transcriptional regulation
Feedback Inhibition in the PhoQ/PhoP Signaling System by a Membrane Peptide
The PhoQ/PhoP signaling system responds to low magnesium and the presence of certain cationic antimicrobial peptides. It regulates genes important for growth under these conditions, as well as additional genes important for virulence in many gram-negative pathogens. PhoQ is a sensor kinase that phosphorylates and activates the transcription factor PhoP. Since feedback inhibition is a common theme in stress-response circuits, we hypothesized that some members of the PhoP regulon may play such a role in the PhoQ/PhoP pathway. We therefore screened for PhoP-regulated genes that mediate feedback in this system. We found that deletion of mgrB (yobG), which encodes a 47 amino acid peptide, results in a potent increase in PhoP-regulated transcription. In addition, over-expression of mgrB decreased transcription at both high and low concentrations of magnesium. Localization and bacterial two-hybrid studies suggest that MgrB resides in the inner-membrane and interacts directly with PhoQ. We further show that MgrB homologs from Salmonella typhimurium and Yersinia pestis also repress PhoP-regulated transcription in these organisms. In cell regulatory circuits, feedback has been associated with modulating the induction kinetics and/or the cell-to-cell variability in response to stimulus. Interestingly, we found that elimination of MgrB-mediated feedback did not have a significant effect on the kinetics of reporter protein production and did not decrease the variability in expression among cells. Our results indicate MgrB is a broadly conserved membrane peptide that is a critical mediator of negative feedback in the PhoQ/PhoP circuit. This new regulator may function as a point of control that integrates additional input signals to modulate the activity of this important signaling system
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