688 research outputs found
The aerodynamics of electric arcs in axial flow
This thesis investigates the behaviour of a d.c. electric
arc under the influence of a number of different axial gas flow
conditions. The work is directed towards obtaining a greater
understanding of the electric arcs produced within gas blast
circuit breakers. In order to eliminate as many variables as
possible, detailed experiments have been carried out in constant
pressure, subsonic nitrogen flow provided by a low Mach number
shock tube. This facility enables the flow velocity and pressure
to be varied independently, thus allowing their effect on arc
behaviour to be studied separately. A simplified theoretical
model of the arc in this flow field has been developed which gives
excellent agreement with the shock tube experiments. The solution
of the theoretical model is obtained in non-dimensional terms as a
universal characteristic for the constant pressure axial flow arc
The Role of Peripheral Resistance in Controlling Cardiac Output During Exercise
journal articleBiomedical Informatic
Regulation of Cardiac Output During Transition From Rest to Exercise
Conference PaperBiomedical Informatic
The K186E amino acid substitution in the canine influenza virus H3N8 NS1 protein restores its ability to inhibit host gene expression
Canine influenza viruses (CIVs) are the causative agents of canine influenza, a contagious respiratory disease in dogs, and include the equine-origin H3N8 and the avian-origin H3N2. Influenza A virus (IAV) non-structural protein 1 (NS1) is a virulence factor essential for counteracting the innate immune response. Here, we evaluated the ability of H3N8 CIV NS1 to inhibit host innate immune responses. We found that H3N8 CIV NS1 was able to efficiently counteract interferon (IFN) responses but was unable to block general gene expression in human or canine cells. Such ability was restored by a single amino acid substitution in position 186 (K186E) that resulted in NS1 binding to the 30-kDa subunit of the cleavage and polyadenylation specificity factor (CPSF30), a cellular protein involved in pre-mRNA processing. We also examined the frequency distribution of K186 and E186 among H3N8 CIVs and equine influenza viruses (EIVs), the ancestors of H3N8 CIV, and experimentally determined the impact of amino acid 186 in the ability of different CIV and EIV NS1s to inhibit general gene expression. In all cases, the presence of E186 was responsible for the control of host gene expression. Contrastingly, the NS1 protein of H3N2 CIV harbors E186 and blocks general gene expression in canine cells. Altogether, our results confirm previous studies on the strain-dependent ability of NS1 to block general gene expression. Moreover, the observed polymorphism on amino acid 186 between H3N8 and H3N2 CIVs might be the result of adaptive changes acquired during long-term circulation of avian-origin IAVs in mammals.
IMPORTANCE: Canine influenza is a respiratory disease of dogs caused by two CIV subtypes, the H3N8 and H3N2 viruses of equine and avian origin, respectively. Influenza NS1 is the main viral factor responsible for the control of host innate immune responses and changes in NS1 can play an important role in host adaptation. Here we assessed the ability of H3N8 CIV NS1 to inhibit host innate immune responses and gene expression. The H3N8 CIV NS1 did not block host gene expression but this activity was restored by a single amino acid substitution (K186E), which was responsible for NS1 binding to the host factor CPSF30. In contrast, the H3N2 CIV NS1, that contains E186, blocks general gene expression. Our results suggest that the ability to block host gene expression is not required for influenza replication in mammals but might be important in the long-term adaptation of avian-origin influenza viruses to mammals
Introduction
Cambridge Studies in Nineteenth-Century Literature and Culture No. 45Copyright © 2004 Cambridge University PressFor the Victorian reading public, periodicals played a far greater role than books in shaping their understanding of new discoveries and theories in science, technology and medicine. Such understandings were formed not merely by serious scientific articles, but also by glancing asides in political reports, fictional representations, or humorous attacks in comic magazines. Ranging across diverse forms of periodicals, from top-selling religious and juvenile magazines through to popular fiction-based periodicals, and from the campaigning 'new journalism' of the late century to the comic satire of Punch, this book explores the ways in which scientific ideas and developments were presented to a variety of Victorian audiences. In addition, it offers three case studies of the representation of particular areas of science: 'baby science', scientific biography, and electricity. This intriguing collaborative volume sheds light on issues relating to history and history of science, literature, book history, and cultural and media studies
Magnetic susceptibility of alkali-TCNQ salts and extended Hubbard models with bond order and charge density wave phases
The molar spin susceptibilities of Na-TCNQ, K-TCNQ and Rb-TCNQ(II)
are fit quantitatively to 450 K in terms of half-filled bands of three
one-dimensional Hubbard models with extended interactions using exact results
for finite systems. All three models have bond order wave (BOW) and charge
density wave (CDW) phases with boundary for nearest-neighbor
interaction and on-site repulsion . At high , all three salts have
regular stacks of anion radicals. The fits place Na and
K in the CDW phase and Rb(II) in the BOW phase with . The Na and
K salts have dimerized stacks at while Rb(II) has regular stacks at
100K. The analysis extends to dimerized stacks and to dimerization
fluctuations in Rb(II). The three models yield consistent values of ,
and transfer integrals for closely related stacks. Model
parameters based on are smaller than those from optical data that in
turn are considerably reduced by electronic polarization from quantum chemical
calculation of , and on adjacent ions. The
analysis shows that fully relaxed states have reduced model parameters compared
to optical or vibration spectra of dimerized or regular stacks.Comment: 9 pages and 5 figure
A temperature sensitive live-attenuated canine influenza virus H3N8 vaccine
Canine influenza is a respiratory disease of dogs caused by canine influenza virus (CIV). CIV subtypes responsible for influenza in dogs include H3N8, which originated from the transfer of H3N8 equine influenza virus to dogs; and the H3N2 CIV, which is an avian-origin virus that adapted to infect dogs. Influenza infections are most effectively prevented through vaccination to reduce transmission and future infection. Currently, only inactivated influenza vaccines (IIVs) are available for the prevention of CIV in dogs. However, the efficacy of IIVs is suboptimal, and novel approaches are necessary for the prevention of disease caused by this canine respiratory pathogen. Using reverse genetics techniques, we have developed a live-attenuated CIV vaccine (LACIV) for the prevention of H3N8 CIV. The H3N8 LACIV replicates efficiently in canine cells at 33°C but is impaired at temperatures of 37 to 39°C and was attenuated compared to wild-type H3N8 CIV in vivo and ex vivo. The LACIV was able to induce protection against H3N8 CIV challenge with a single intranasal inoculation in mice. Immunogenicity and protection efficacy were better than that observed with a commercial CIV H3N8 IIV but provided limited cross-reactive immunity and heterologous protection against H3N2 CIV. These results demonstrate the feasibility of implementing a LAIV approach for the prevention and control of H3N8 CIV in dogs and suggest the need for a new LAIV for the control of H3N2 CIV.
Importance: Two influenza A virus subtypes has been reported in dogs in the last 16 years: the canine influenza viruses (CIV) H3N8 and H3N2 of equine and avian origins, respectively. To date, only inactivated influenza vaccines (IIVs) are available to prevent CIV infections. Here, we report the generation of a recombinant, temperature-sensitive H3N8 CIV as a live-attenuated influenza vaccine (LAIV), which was attenuated in mice and dog tracheal, explants compared to CIV H3N8 wild type. A single dose of H3N8 LACIV showed immunogenicity and protection against a homologous challenge that was better than that conferred with an H3N8 IIV, demonstrating the feasibility of implementing a LAIV approach for the improved control of H3N8 CIV infections in dogs
Hydrogen bonding in acrylamide and its role in the scattering behavior of acrylamide-based block copolymers
Hydrogen bonding plays a role in the microphase separation behavior of many block copolymers, such as those used in lithography, where the stronger interactions due to H-bonding can lead to a smaller period for the self-assembled structures, allowing the production of higher resolution templates. However, current statistical thermodynamic models used in descriptions of microphase separation, such as the Flory-Huggins approach, do not take into account some important properties of hydrogen bonding, such as site specificity and cooperativity. In this combined theoretical and experimental study, a step is taken toward the development of a more complete theory of hydrogen bonding in polymers, using polyacrylamide as a model system. We begin by developing a set of association models to describe hydrogen bonding in amides. Both models with one association constant and two association constants are considered. This theory is used to fit IR spectroscopy data from acrylamide solutions in chloroform, thereby determining the model parameters. These parameters are then employed to calculate the scattering function of the disordered state of a diblock copolymer with one polyacrylamide block and one non-hydrogen-bonding block in the random phase approximation. It is then shown that the expression for the inverse scattering function with hydrogen bonding is the same as that without hydrogen bonding, but with the Flory-Huggins parameter χ replaced by an effective value χeff=χ+δχHB(f), where the hydrogen-bonding contribution δχHB depends on the volume fraction f of the hydrogen-bonding block. We find that models with two constants give better predictions of bond energy in the acrylamide dimer and more realistic asymptotic behavior of the association constants and δχHB in the limit of high temperatures
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