An Allosteric Transcription Factor DNA-Binding Electrochemical Biosensor for Progesterone

We describe an electrochemical strategy to transduce allosteric transcription factor (aTF) binding affinity to sense steroid hormones. Our approach utilizes square wave voltammetry to monitor changes in current output as a progesterone (PRG)-specific aTF (SRTF1) unbinds from the cognate DNA sequence in the presence of PRG. The sensor detects PRG in artificial urine samples with sufficient sensitivity suitable for clinical applications. Our results highlight the capability of using aTFs as the biorecognition elements to develop electrochemical point-of-care biosensors for the detection of small-molecule biomarkers and analytes

Microvesicles transfer mitochondria and increase mitochondrial function in brain endothelial cells

We have demonstrated, for the first time that microvesicles, a sub-type of extracellular vesicles (EVs) derived from hCMEC/D3: a human brain endothelial cell (BEC) line transfer polarized mitochondria to recipient BECs in culture and to neurons in mice acute brain cortical and hippocampal slices. This mitochondrial transfer increased ATP levels by 100 to 200-fold (relative to untreated cells) in the recipient BECs exposed to oxygen-glucose deprivation, an in vitro model of cerebral ischemia. We have also demonstrated that transfer of microvesicles, the larger EV fraction, but not exosomes resulted in increased mitochondrial function in hypoxic endothelial cultures. Gene ontology and pathway enrichment analysis of EVs revealed a very high association to glycolysis-related processes. In comparison to heterotypic macrophage-derived EVs, BEC-derived EVs demonstrated a greater selectivity to transfer mitochondria and increase endothelial cell survival under ischemic conditions

Microvesicles Transfer Mitochondria and Increase Mitochondrial Function in Brain Endothelial Cells

AbstractWe have demonstrated, for the first time that microvesicles, a sub-type of extracellular vesicles (EVs) derived from hCMEC/D3: a human brain endothelial cell (BEC) line transfer polarized mitochondria to recipient BECs in culture and to neurons in mice acute brain cortical and hippocampal slices. This mitochondrial transfer increased ATP levels by 100 to 200-fold (relative to untreated cells) in the recipient BECs exposed to oxygen-glucose deprivation, an in vitro model of cerebral ischemia. We have also demonstrated that transfer of microvesicles, the larger EV fraction, but not exosomes resulted in increased mitochondrial function in hypoxic endothelial cultures. Gene ontology and pathway enrichment analysis of EVs revealed a very high association to glycolysis-related processes. In comparison to heterotypic macrophage- derived EVs, BEC-derived EVs demonstrated a greater selectivity to transfer mitochondria and increase endothelial cell survival under ischemic conditions.HighlightsMicrovesicles transfer mitochondria to endothelial cells and brain slice neuronsMitochondrial transfer increased ATP in ischemic brain endothelial cells (BECs)Transfer of microvesicles increased mitochondrial function in hypoxic BECsTransfer of exosomes did not affect mitochondrial function in hypoxic BECsHomotypic BEC-derived EVs result in greater ATP levels in the recipient BECs</jats:sec

Merely a transit country? Examining the role of Uganda in the transnational illegal ivory trade

This is the final text version of the article, it may contain minor differences from the publisher’s pdf version.Uganda is repeatedly implicated in the illegal ivory trade as a transit territory for ivory destined for Asia. Interviews with law enforcement officers reveal that the size of seizures and means of concealment and transportation are varied, showing diversity in the trade’s level of organization and sophistication. Arguably, considerable processing takes place within Uganda in terms of stockpiling, repacking and organization of exports but investigations rarely lead to prosecution and conviction of those responsible. Findings demonstrate the intricate role and responsibility of transit countries and illustrate how the transnational ivory trade operates locally