1,491 research outputs found
High Spectral and Spatial Resolution Observations of the PDR Emission in the NGC2023 Reflection Nebula with SOFIA and APEX
We have mapped the NGC 2023 reflection nebula in [CII] and CO(11--10) with
the heterodyne receiver GREAT on SOFIA and obtained slightly smaller maps in
13CO(3--2), CO(3--2), CO(4--3), CO(6--5), and CO(7--6) with APEX in Chile. We
use these data to probe the morphology, kinematics, and physical conditions of
the C II region, which is ionized by FUV radiation from the B2 star HD37903.
The [CII] emission traces an ellipsoidal shell-like region at a position angle
of ~ -50 deg, and is surrounded by a hot molecular shell. In the southeast,
where the C II region expands into a dense, clumpy molecular cloud ridge, we
see narrow and strong line emission from high-J CO lines, which comes from a
thin, hot molecular shell surrounding the [CII] emission. The [CII] lines are
broader and show photo evaporating gas flowing into the C II region. Based on
the strength of the [13CII] F=2--1 line, the [CII] line appears to be somewhat
optically thick over most of the nebula with an optical depth of a few. We
model the physical conditions of the surrounding molecular cloud and the PDR
emission using both RADEX and simple PDR models. The temperature of the CO
emitting PDR shell is ~ 90 -- 120 K, with densities of 10^5 -- 10^6 cm^-3, as
deduced from RADEX modeling. Our PDR modeling indicates that the PDR layer
where [CII] emission dominates has somewhat lower densities, 10^4 to a few
times 10^5 cm^-3Comment: Accepted by A&
Giant Angiolymphoid Hyperplasia with Eosinophilia on the Chest
Angiolymphoid hyperplasia with eosinophilia is a rare vascular proliferation characterized by single or multiple purplish, brownish papules and subcutaneous nodules, sometimes associated with pain or pruritus. This rare benign process occurs with a female predominance. Approximately 85% of the lesions occur in the skin of the head and neck; most of them are around the ear or on the forehead or scalp. Whether angiolymphoid hyperplasia with eosinophilia represents a benign neoplasm or an unusual reaction to varied stimuli, including trauma, the etiology remains unclear. Histopathologically, the lesions consist of a proliferation of blood vessels of variable size lined by large epithelioid endothelial cells and a variable inflammatory infiltrate of lymphocytes and eosinophils, sometimes with lymphoid follicle formation. The lesion is benign but may be persistent and is difficult to eradicate. We report on a case of a 58-year-old Caucasian man who presented a purplish pink dome-shaped tumor of size up to 8 cm in diameter located on the chest. We emphasize this case considering the unusual dimensions of the lesion (8 cm diameter) and the atypical location on the chest
Disentangling the excitation conditions of the dense gas in M17 SW
We probe the chemical and energetic conditions in dense gas created by
radiative feedback through observations of multiple CO, HCN and HCO
transitions toward the dense core of M17 SW. We used the dual band receiver
GREAT on board the SOFIA airborne telescope to obtain maps of the ,
, and transitions of CO. We compare these maps with
corresponding APEX and IRAM 30m telescope data for low- and mid- CO, HCN and
HCO emission lines, including maps of the HCN and HCO
transitions. The excitation conditions of CO, HCO and HCN are
estimated with a two-phase non-LTE radiative transfer model of the line
spectral energy distributions (LSEDs) at four selected positions. The energy
balance at these positions is also studied. We obtained extensive LSEDs for the
CO, HCN and HCO molecules toward M17 SW. The LSED shape, particularly the
high- tail of the CO lines observed with SOFIA/GREAT, is distinctive for the
underlying excitation conditions. The critical magnetic field criterion implies
that the cold cloudlets at two positions are partially controlled by processes
that create and dissipate internal motions. Supersonic but sub-Alfv\'enic
velocities in the cold component at most selected positions indicates that
internal motions are likely MHD waves. Magnetic pressure dominates thermal
pressure in both gas components at all selected positions, assuming random
orientation of the magnetic field. The magnetic pressure of a constant magnetic
field throughout all the gas phases can support the total internal pressure of
the cold components, but it cannot support the internal pressure of the warm
components. If the magnetic field scales as , then the
evolution of the cold cloudlets at two selected positions, and the warm
cloudlets at all selected positions, will be determined by ambipolar diffusion.Comment: 26 pages, 13 figures, A&A accepte
Radiative and mechanical feedback into the molecular gas of NGC 253
Starburst galaxies are undergoing intense periods of star formation.
Understanding the heating and cooling mechanisms in these galaxies can give us
insight to the driving mechanisms that fuel the starburst. Molecular emission
lines play a crucial role in the cooling of the excited gas. With SPIRE on the
Herschel Space Observatory we have observed the rich molecular spectrum towards
the central region of NGC 253. CO transitions from J=4-3 to 13-12 are observed
and together with low-J line fluxes from ground based observations, these lines
trace the excitation of CO. By studying the CO excitation ladder and comparing
the intensities to models, we investigate whether the gas is excited by UV
radiation, X-rays, cosmic rays, or turbulent heating. Comparing the CO
and CO observations to large velocity gradient models and PDR models we
find three main ISM phases. We estimate the density, temperature,and masses of
these ISM phases. By adding CO, HCN, and HNC line intensities, we are
able to constrain these degeneracies and determine the heating sources. The
first ISM phase responsible for the low-J CO lines is excited by PDRs, but the
second and third phases, responsible for the mid to high-J CO transitions,
require an additional heating source. We find three possible combinations of
models that can reproduce our observed molecular emission. Although we cannot
determine which of these are preferable, we can conclude that mechanical
heating is necessary to reproduce the observed molecular emission and cosmic
ray heating is a negligible heating source. We then estimate the mass of each
ISM phase; M for phase 1 (low-J CO lines), M for phase 2 (mid-J CO lines), and M for
phase 3 (high-J CO lines) for a total system mass of M
The thermal state of molecular clouds in the Galactic Center: evidence for non-photon-driven heating
We used the Atacama Pathfinder Experiment (APEX) 12 m telescope to observe
the J_KaKc=3_03-2_02, 3_22-2_21, and 3_21-2_20 transitions of para-H_2CO at 218
GHz simultaneously to determine kinetic temperatures of the dense gas in the
central molecular zone (CMZ) of our Galaxy. The map extends over approximately
40 arcmin x 8 arcmin (~100x20 pc^2) along the Galactic plane with a linear
resolution of 1.2 pc. The strongest of the three lines, the H_2CO (3_03-2_02)
transition, is found to be widespread, and its emission shows a spatial
distribution similar to ammonia. The relative abundance of para-H_2CO is
0.5-1.2 10^{-9}, which is consistent with results from lower frequency H_2CO
absorption lines. Derived gas kinetic temperatures for individual molecular
clouds range from 50 K to values in excess of 100 K. While a systematic trend
toward (decreasing) kinetic temperature versus (increasing) angular distance
from the Galactic center (GC) is not found, the clouds with highest temperature
(T_kin > 100 K) are all located near the nucleus. For the molecular gas outside
the dense clouds, the average kinetic temperature is 65+/-10 K. The high
temperatures of molecular clouds on large scales in the GC region may be driven
by turbulent energy dissipation and/or cosmic-rays instead of photons. Such a
non-photon-driven thermal state of the molecular gas provides an excellent
template for the more distant vigorous starbursts found in ultraluminous
infrared galaxies (ULIRGs).Comment: 23 pages, 11 figures, A&A in pres
Abundant Z-cyanomethanimine in the interstellar medium: paving the way to the synthesis of adenine
We report the first detection in the interstellar medium of the Z-isomer of
cyanomethanimine (HNCHCN), an HCN dimer proposed as precursor of adenine. We
identified six transitions of Z-cyanomethanimine, along with five transitions
of E-cyanomethanimine, using IRAM 30m observations towards the Galactic Center
quiescent molecular cloud G+0.693. The Z-isomer has a column density of
(2.00.6)10 cm and an abundance of
1.510. The relative abundance ratio between the isomers is
[Z/E]6. This value cannot be explained by the two chemical formation
routes previously proposed (gas-phase and grain surface), which predicts
abundances ratios between 0.9 and 1.5. The observed [Z/E] ratio is in good
agreement with thermodynamic equilibrium at the gas kinetic temperature
(130210 K). Since isomerization is not possible in the ISM, the two species
may be formed at high temperature. New chemical models, including surface
chemistry on dust grains and gas-phase reactions, should be explored to explain
our findings. Whatever the formation mechanism, the high abundance of Z-HNCHCN
shows that precursors of adenine are efficiently formed in the ISM.Comment: Accepted in Monthly Notices of the Royal Astronomical Society Letter
Complex organic molecules in the Galactic Centre: the N-bearing family
We present an unbiased spectral line survey toward the Galactic Centre (GC)
quiescent giant molecular cloud (QGMC), G+0.693 using the GBT and IRAM 30
telescopes. Our study highlights an extremely rich organic inventory of
abundant amounts of nitrogen (N)-bearing species in a source without signatures
of star formation. We report the detection of 17 N-bearing species in this
source, of which 8 are complex organic molecules (COMs). A comparison of the
derived abundances relative to H is made across various galactic and
extragalactic environments. We conclude that the unique chemistry in this
source is likely to be dominated by low-velocity shocks with X-rays/cosmic rays
also playing an important role in the chemistry. Like previous findings
obtained for O-bearing molecules, our results for N-bearing species suggest a
more efficient hydrogenation of these species on dust grains in G+0.693 than in
hot cores in the Galactic disk, as a consequence of the low dust temperatures
coupled with energetic processing by X-ray/cosmic ray radiation in the GC.Comment: 24 pages, 23 figures, 7 tables, accepted for publication in MNRA
In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system
<p>Abstract</p> <p>Background</p> <p>Due to its extremely high strength, the interaction between biotin and (strept)avidin has been exploited for a large number of biotechnological applications. Site-specific biotinylation of proteins <it>in vivo </it>can be achieved by co-expressing in mammalian cells the protein of interest fused to a 15 amino acid long Biotin Acceptor Peptide (BAP) and the bacterial biotin-protein ligase BirA, which specifically recognizes and attaches a biotin to the single lysine residue of the BAP sequence. However, this system is mainly based on the contemporaneous use of two different plasmids or on induction of expression of two proteins through an IRES-driven mechanism.</p> <p>Results</p> <p>We developed a single bigenic plasmid that contains two independent transcriptional units for the co-expression of both the protein tagged with BAP and an engineered version of the BirA enzyme. Upstream of the cDNA encoding BirA, a signal secretion leader sequence was added to allow translocation of the enzyme to the secretory pathway. Three different recombinant antibodies in the scFv format, a membrane bound and secretory truncated IgE Fc fragment and a soluble version of the human IgE high affinity receptor were shown to be efficiently biotinylated and to maintain their binding properties in immunofluorescence microscopy, flow cytometry and ELISA assays.</p> <p>Conclusion</p> <p>The present study shows the universal applicability to both secretory and membrane bound proteins of a single bigenic plasmid to induce the site-specific <it>in vivo </it>biotinylation of target molecules tagged with a short acceptor peptide. These molecules could be easily obtained from supernatants or extracts of mammalian cells and used for a wide range of biological applications.</p
Chemical Features in the Circumnuclear Disk of the Galactic Center
The circumnuclear disk (CND) of the Galactic Center is exposed to many
energetic phenomena coming from the supermassive black hole Sgr A* and stellar
activities. These energetic activities can affect the chemical composition in
the CND by the interaction with UV-photons, cosmic-rays, X-rays, and shock
waves. We aim to constrain the physical conditions present in the CND by
chemical modeling of observed molecular species detected towards it. We
analyzed a selected set of molecular line data taken toward a position in the
southwest lobe of the CND with the IRAM 30m and APEX 12-meter telescopes and
derived the column density of each molecule using a large velocity gradient
(LVG) analysis. The determined chemical composition is compared with a
time-dependent gas-grain chemical model based on the UCL\_CHEM code that
includes the effects of shock waves with varying physical parameters. Molecules
such as CO, HCN, HCO, HNC, CS, SO, SiO, NO, CN, HCO, HCN,
NH and HO are detected and their column densities are obtained.
Total hydrogen densities obtained from LVG analysis range between and cm and most species indicate values around
several cm, which are lower than values corresponding to
the Roche limit, which shows that the CND is tidally unstable. The chemical
models show good agreement with the observations in cases where the density is
cm, the cosmic-ray ionization rate is high, s, or shocks with velocities km s have occurred.
Comparison of models and observations favors a scenario where the cosmic-ray
ionization rate in the CND is high, but precise effects of other factors such
as shocks, density structures, UV-photons and X-rays from the Sgr A* must be
examined with higher spatial resolution data.Comment: 17 Pages, 13 figures, accepted for publication in A&
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