Gene delivery by negatively charged ternary complexes of DNA, cationic liposomes and transferrin or fusigenic peptides

Potential problems with the use of viral vectors for gene therapy necessitate the development of efficient nonviral vectors. The association of transferrin, or the pH-sensitive peptide GALA, with cationic liposomes composed of 1,2-dioleoyl-3-(trimethylammonium) propane and its equimolar mixture with dioleoylphosphatidylethanolamine, under conditions where the liposome/DNA complex is negatively charged, drastically increased luciferase expression from pCMVluc. The percentage of cells transfected, measured by β-galactosidase expression, was also increased by about 10-fold. The zeta potential of the ternary complexes was lower than that of the liposome/DNA complexes. Transfection activity of positively charged complexes was also enhanced by association with transferrin, GALA or the influenza hemagglutinin N terminal peptide HA-2, but to a smaller extent compared with the negatively charged complexes. The enhancement of gene delivery by transferrin or GALA was not affected significantly by the presence of serum and did not cause significant cytotoxicity. Our results indicate that negatively charged ternary complexes of cationic liposomes, DNA and transferrin, or fusigenic peptides, can facilitate efficient transfection of cultured cells, and that they may alleviate the drawbacks of the use of highly positively charged complexes for gene delivery in vivo

Padronização da codificação programas em Borland Delphi: experiência no projeto SIGI.

Regras gerais para formatação de código fonte: idioma dos nomes; identação; margens; comentários. Linguagem Object Pascal. Tratamento de exceções estruturado. Classes: nomenclatura; atributos; propriedades. Arquivos: arquivos de projeto (project); Arquivos de formulários (Forms); Arquivos de units; Units de formulários; Units dos módulos de dados (data module); Units de propósito geral. Formulários (Forms) e Módulo de dados (Data Modules).bitstream/CNPTIA/9195/1/COMNICADOTEC17_0.pdfAcesso em: 29 maio 2008

Impactos da crise do coronavírus nas cadeias produtivas de frutas e hortaliças brasileiras.

Informações sobre o que vem ocorrendo em vários subsetores da economia brasileira. A Revista de Economia e Agronegócios optou por refletir sobre os principais problemas decorrentes da atual crise relacionados com um importante subsetor da economia agrícola brasileira, o de frutas e hortaliças, objeto de pesquisa dos autores do presente documento. Dessa forma, o que é aqui apresentado é resultado da interpretação conjunta dos autores sobre as informações coletadas em bases de dados das Centrais de Abastecimento e em entrevistas realizadas junto aos diversos agentes das cadeias produtivas de frutas e hortaliças sobre a crise em questão

O uso do lúdico no ensino de química para deficientes visuais ou baixa visão: utilização da tabela FD14

Anais do II Seminário Seminário Estadual PIBID do Paraná: tecendo saberes / organizado por Dulcyene Maria Ribeiro e Catarina Costa Fernandes — Foz do Iguaçu: Unioeste; Unila, 2014A tabela Periódica organizada e elaborada com características puramente visuais como formato, distribuição, cor, símbolos, informações numéricas e propriedades com seguimentos distintos, acaba por excluir de uma vez o deficiente visual. A tabela FD14 ® apresenta uma proposta onde o aluno, poderá coletar todas as informações contidas em uma tabela periódica em formato de jogo, sendo a mesma, o principal instrumento na aprendizagem da química. O modelo de ferramenta proposto foi utilizado por um aluno do 1o ano do Ensino Médio de 15 anos, deficiente visual desde seu nascimento e inserido no Ensino Médio regular no Col. Est. Eurídes Brandão. Para êxito da proposta, “João” conseguiu identificar sem qualquer tipo de auxílio todas as informações que um aluno visual teria acesso, num processo realmente inclusiv

Fusion activity and inactivation of influenza virus: Kinetics of low pH-induced fusion with cultured cells

The kinetics of fusion of influenza virus (A/PR/8/34) with human promyelocytic leukaemia (HL-60), human T lymphocytic leukaemia (CEM) and murine lymphoma (S49) cells were investigated. Fusion was demonstrated by electron microscopy, and monitored by fluorescence dequenching of octadecylrhodamine incorporated in the virus membrane. Rapid fusion was induced upon mild acidification of the medium. At pH 5, all virus particles were capable of fusing with the cells. The initial rate and the extent of fusion were maximal between pH 4.9 and 5.2 and declined sharply below and above this range. The rate constants of adhesion of influenza virus to cells or erythrocyte ghosts were large, indicating a diffusion-controlled process. The rate constants of fusion of the virus with cells were smaller than those found previously for fusion with various liposomes. Although preincubation of the virus at acidic pH in the absence of target membranes almost completely inactivated the virus in its ability to fuse with erythrocyte ghosts, it reduced the extent of fusion with cultured cells by only 20 to 40%. Kinetic analysis of fusion revealed a mode of inactivation of the virus bound to erythrocyte ghosts or suspension cells, below pH 5.4, different from that of the virus preincubated at low pH without target membranes

MicroRNA deregulation and chemotaxis and phagocytosis impairment in Alzheimer's disease

AbstractIntroductionMononuclear phagocytes play a critical role during Alzheimer's disease (AD) pathogenesis due to their contribution to innate immune responses and amyloid beta (Aβ) clearance mechanisms.MethodsBlood-derived monocytes (BDMs) and monocyte-derived macrophages (MDMs) were isolated from blood of AD, mild cognitive impairment (MCI) patients, and age-matched healthy controls for molecular and phenotypic comparisons.ResultsThe chemokine/chemokine receptor CCL2/CCR2 axis was impaired in BDMs from AD and MCI patients, causing a deficit in cell migration. Changes were also observed in MDM-mediated phagocytosis of Aβ fibrils, correlating with alterations in the expression and processing of the triggering receptor expressed on myeloid cells 2 (TREM2). Finally, immune-related microRNAs (miRNAs), including miR-155, -154, -200b, -27b, and -128, were found to be differentially expressed in these cells.DiscussionThis work provides evidence that chemotaxis and phagocytosis, two crucial innate immune functions, are impaired in AD and MCI patients. Correlations with miRNA levels suggest an epigenetic contribution to systemic immune dysfunction in AD