Estudos estruturais das enzimas envolvidas com o mecanismo de produção de ácidos orgânicos da bactéria G. Diazotrophicus utilizando métodos computacionais.

Ga5DH é uma enzima que exerce um papel fundamental na regulação do fluxo das fontes de carbono e energia nas bactérias, e na produção de Ácidos Orgânicos, entre eles o 5-Ceto-D-Gluconato (5KGA) [1,2]. Devido à importância deste ácido na indústria química [3], há um grande interesse em sua estrutura. Isto nos leva a apresentar a estrutura teórica de Ga5DH de Gluconacetobacter diazotrophicus, obtida com Modelagem por Homologia. Dez modelos foram gerados no programa MODELLER [4], baseados na estrutura de Gluconate 5- dehydrogenase de Streptococcus suis (PDB 3cxr), a única proteína Ga5DH resolvida experimentalmente até então.Trabalho apresentado na V Mostra de Trabalhos de Estagiários e Bolsistas, Campinas, out. 2009

EVALUATION OF THE EFFECTS OF ILLUMINATION, TEMPERATURE AND PAINTING FINISH IN PSP TECHNIQUE

This paper reports the results of global pressure measurements on a NACA0012 profile, obtained with the Pressure Sensitive Paint technique (PSP), and compares them with conventional pressure tap measurements with the purpose of analyzing the influence of parameters as illumination efficiency and temperature variation of the air flow in the accuracy of the PSP experimental results. The experimental measurements were conducted in the Pilot Transonic Wind Tunnel (TTP) of the Instituto de Aeronáutica e Espaço (IAE) for Mach number values of 0.4, 0.6 and 0.8. The experiments showed that in a carefully prepared experiment, under appropriated operational conditions, and rigorous adjustments of the PSP components, the obtained results can be accurate. Moreover, even when the influence of temperature is significant and the illumination condition is not ideal, it was possible to estimate well the impact caused in the pressure distribution over the model surface

Emerging biotechnologies: bioinformatics services applied to agriculture.

Abstract - Bioinformatics is an emergent biotechnological field of study marked by interdisciplinarity and complexity. It involves the application and development of computational tools to biological data in order to process, generate, and disseminate biological knowledge. Bioinformatics is characterized by an intense generation of data and information (configured as a context of big data and e-science), associated with the need for computational resources with high processing and storage capacities and highly qualified and interdisciplinary staff, often found only in academia. The objective of this paper is to describe the organizational model and collaborative innovation activities of the Bioinformatics Multi-user Laboratory (LMB, in the acronym in Portuguese). The LMB is a facility located at the Brazilian Agricultural Research Corporation (Embrapa), the main Brazilian agricultural research public institute, formed by 46 Research and Service Centers distributed throughout Brazil and by several laboratories and business offices abroad, in America, Africa, Asia and Europe. Its mission involves to contribute to the advance of the frontier of knowledge in bioinformatics by: incorporating new technologies and enabling efficient solutions to the demands related to this field; providing access to high performance computing infrastructure and developing human skills. Considering the importance of biotechnology in the context of agricultural research, Embrapa implemented the LMB in 2011, with the purpose of increasing the efficiency of the use of computational, human and technological resources of Embrapa by providing access to bioinformatics computational resources, offering research collaboration possibilities and consultation on project design and biological data analysis. A case-study was conducted based on documentary research and interviews. The main findings of this research are: the description of the organizational model of LMB, the management team and roles; theservices it provides; its access policies and procedures of customer service.Altec 2015

Antimicrobial and plant growth-promoting properties of the cacao endophyte Bacillus subtilis ALB629.

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Genética da resistência de planta adulta à ferrugem da folha em trigo - cultivar Toropi.

Orientadora: Sandra Patussi Brammer

Structural and optical characterization of Mg-doped GaAs nanowires grown on GaAs and Si substrates

We report an investigation on the morphological, structural, and optical properties of large size wurtzite GaAs nanowires, low doped with Mg, grown on GaAs(111) B and Si(111) substrates. A higher density of vertical nanowires was observed when grown upon GaAs(111) B. Very thin zinc-blende segments are observed along the axis of the nanowires with a slightly higher linear density being found on the nanowires grown on Si(111). Low temperature cathodoluminescence and photoluminescence measurements reveal an emission in the range 1.40-1.52 eV related with the spatial localization of the charge carriers at the interfaces of the two crystalline phases. Mg related emission is evidenced by cathodoluminescence performed on the GaAs epilayer. However, no direct evidence for a Mg related emission is found for the nanowires. The excitation power dependency on both peak energy and intensity of the photoluminescence gives a clear evidence for the type II nature of the radiative transitions. From the temperature dependence on the photoluminescence intensity, non-radiative de-excitation channels with different activation energies were found. The fact that the estimated energies for the escape of the electron are higher in the nanowires grown on Si(111) suggests the presence of wider zinc-blende segments. (C) 2013 AIP Publishing LLC