The effect of protozoa on the bacterial composition and hydrolytic activity of the roe deer rumen.

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The purpose of this study was to compare the effect of the presence of protozoa in the rumen of wild roe deer (Capreolus capreolus) on the bacteria composition and digestion rate of the main carbohydrates of forage. The research material involved rumen content and rumen fluid, which were collected in the autumn-winter season, from eight adult males of roe deer with an average body mass of 22.6 kg. The microscopic analysis demonstrated that there were only protozoa in 50% of the animals sampled. Qualitative analysis revealed the presence of protozoa belonging to the genus Entodinium. The density of protozoal population varied from 6.5 to 38.7 × 105/mL rumen fluid. The analysis of bacteria composition indicated that protozoa did not have an effect on bacterial diversity. Furthermore, the results of hydrolytic activity revealed that the fastest digestion of carbohydrates was for pectin, while the slowest was inulin. The pH and redox potential in the rumen varied from 5.9 to 6.1 and from −248.1 to −251.1 mV, respectively. In summary, the presence of protozoa in the rumen of wild roe deer does not have an effect on the bacterial population and has no effect on the digestion rate of carbohydrates in the rumen

Effect of seasonal diet composition changes on the characteristics of the gastrointestinal tract contents of the Eurasian beaver (Castor fiber).

The goal of the study was to compare chemical and physical parameters of the content of various gastrointestinal (GI) tract segments of adult beavers and to relate the obtained results to seasonal changes in the composition of natural diet. The study was conducted on 36 adult beavers originated from the natural environment that were captured in winter, summer and autumn. The obtained results demonstrated that pH, redox potential (Eh), dry matter (DM), short-chain fatty acids (SCFA) and distribution of food particles varied along the beaver GI tract during the year. The lowest value of pH was observed in stomach whereas the highest in the small intestine and cecum. The reverse pattern of changes was found for the value of redox potential. The research indicated that anaerobic conditions were predominant in the cecum and so contributed to development of microbial population. Also, the increase in the SCFA contents in the cecum and colon indicated that beavers belong both to hindgut fermenters as well as to the mixed moderate type animals. In summary, the obtained results indicated that seasonal changes in the diet composition affected physical and chemical parameters as well as microbial activity of the GI tract of beaver

Effect of Entodinium caudatum on starch intake and glycogen formation by Eudiplodinium maggii in the rumen and reticulum.

This study aimed to quantify the engulfed starch and reserve α-glucans (glycogen) in the cells of the ciliates Eudiplodinium maggii, as well the α-glucans in defaunated and selectively faunated sheep. The content of starch inside the cell of ciliates varied from 21 to 183 mg/g protozoal DM relative to the rumen fauna composition whereas, the glycogen fluctuated between 17 and 126 mg/g dry matter (DM) of this ciliate species. Establishment of the population Entodinium caudatum in the rumen of sheep already faunated with E. maggii caused a drop in both types of quantified carbohydrates. The content of α-glucans in the rumen of defaunated sheep varied from 4.4 to 19.9 mg/g DM and increased to 7.4–29.9 or 11.8–33.9 mg/g DM of rumen contents in the presence of only E. maggii or E. maggii and E. caudatum, respectively. The lowest content of the carbohydrates was always found just before feeding and the highest at 4 h thereafter. The α-glucans in the reticulum varied 7.5–40.1, 14.3–76.8 or 21.9–106.1 mg/g DM of reticulum content for defaunated, monofaunated or bifaunated sheep, respectively. The results indicated that both ciliate species engulf starch granules and convert the digestion products to the glycogen, diminishing the pool of starch available for amylolytic bacteria

Influence of ingested forage on the composition of rumen microfauna in fallow deer

The aim of the study was to determine the correlation between ingested forage and composition of microfauna in the rumen of fallow deer. Rumen content of six specimens was obtained from the Bolimowska Forest during 2011/12 hunting season. Protozoa of the Entodinium and Epidinium genera belonging to the Ophryoscoleciade family were identified in analysed samples. Density of Entodinium population was positively correlated with percentage share of forage rich in starch. It was also reversely and equally strongly correlated to the share of forage rich in cellulose (p>0.05). Share of volume and biomass of Epidinium population was correlated negatively with the share of acorns in the forage (p>0.1). Differences in number and share of protozoa of the Entodinium and Epidinium genera in the rumen of fallow deer coexisting in the same place and time indicate feeding opportunism of this ruminant

The influence of surface and speed on biomechanical external loads obtained from wearable devices in rearfoot strike runners

External load variables such as peak tibial acceleration (PTA), peak vertical ground reaction forces (GRF) and its instantaneous vertical loading rate (IVLR) may contribute to running injuries although evidence is conflicting given the influence of training load and tissue health on injuries. These variables are influenced by footwear, speed, surface and foot strike pattern during running. The purpose of this study was to assess the influence of four surfaces and two running speeds on external load variables in rearfoot strike (RFS) runners. Twelve RFS runners (confirmed with sagittal foot contact angle) completed a 2-min running bout on a treadmill and 50-m running bouts over the three surfaces (pavement, rubber track and grass) in standardised shoes at their preferred speed and 20% faster. PTA and vertical GRFs were collected using inertial measurement units and in-shoe force insoles. No interaction or surface effects were observed (p \u3e 0.017). The faster speed produced greater axial PTA (+19.2%; p \u3c 0.001), resultant PTA (+20.7%; p \u3c 0.001), peak vertical GRF (+6.6%; p = 0.002) and IVLR (+16.5%; p \u3c 0.001). These findings suggest that surface type does not influence PTA, peak vertical GRF and IVLR but that running faster increases the magnitude of these external loads regardless of surface type in RFS runners

Are Ophryoscolex caudatus and Ophryoscolex purkynjei two different species of rumen ciliates or only two different forms of the same species?

Item does not contain fulltext4th Symposium on Gut Microbiology, 21 juni 200

Potato Ingestion as an Effective Race Fuel Alternative to Improve Cycling Performance in Trained Cyclists

Carbohydrate (CHO) ingestion is an established strategy to improve endurance performance. Race fuels should not only sustain performance, but also be readily digested and absorbed and replenish electrolytes. Potatoes are a cost-effective option that fulfills these criteria; however, their impact on endurance performance remains unexamined. PURPOSE: Compare the effects of potato purée (POT) ingestion during endurance cycling on subsequent performance versus commercial CHO gel (GEL) or a control (water, CTL). METHODS: Twelve trained cyclists (31±9y; 71±8kg; VO2max: 61±9mL/kg/min) consumed a standardized breakfast then performed a 2h cycling challenge (60-85%VO2max) followed by a time trial (6kJ/kg body mass) while consuming POT, GEL, or CTL in a randomized-crossover design. POT, GEL and CTL were administered with U-[13C6]glucose for an indirect estimate of gastric emptying rate. Repeated blood samples were collected. RESULTS: Time trial performance significantly improved (P=0.03) with POT (33.0±4.5min) and GEL (33.0±4.2min) versus CTL condition (39.5±7.9min); while POT and GEL conditions (P=1.00) had no difference. Post-challenge, blood glucose concentrations were lower (P0.05). CONCLUSION: Potatoes served as a viable alternative to commercial gels by sustaining performance and blood glucose concentrations during endurance cycling events in trained cyclists