193 research outputs found

    A flume experiment on the effect of constriction shape on the formation of forced pools

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    A series of 18 flume runs were conducted in a 6-m long, 0.5-m wide recirculating flume with a bed gradient of 0.8% to determine the influence of obstruction shape on the formation and characteristics of forced pools. Six different-shaped obstructions were added to the flume with the maximum width of the obstruction held constant at 20 cm, which equaled a 40% constriction of flow. The obstruction shapes used included a square, a rectangle, a right triangle with the hypotenuse-facing upstream, a right triangle with the hypotenuse-facing downstream, a combination of a square and triangle with the hypotenuse-facing upstream, and a rectangle and semi-circle shape. Three flume runs were conducted with each obstruction shape. A profile of the flume bed was taken after each experiment and a grid measurement of bed elevations for the last run were conducted to create topographic maps of the flume bed to compare pool-riffle morphologies. ANOVA results indicate pool depth, pool location, and the distance between the pool center and the riffle crest all vary with the obstruction shape. Obstructions with a more blunt upstream face created deeper pools, more total scour and longer pool-riffle sequence lengths than pools formed by obstructions with a more gradual narrowing of flow. The increased volume of scour associated with obstructions that rapidly narrow flow also creates larger volume riffles that cover a greater extent of the channel bed

    A Budding-Defective M2 Mutant Exhibits Reduced Membrane Interaction, Insensitivity To Cholesterol, And Perturbed Interdomain Coupling

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    Influenza A M2 is a membrane-associated protein with a C-terminal amphipathic helix that plays a cholesterol-dependent role in viral budding. An M2 mutant with alanine substitutions in the C-terminal amphipathic helix is deficient in viral scission. With the goal of providing atomic-level understanding of how the wild-type protein functions, we used a multipronged site-directed spin labeling electron paramagnetic resonance spectroscopy (SDSL-EPR) approach to characterize the conformational properties of the alanine mutant. We spin-labeled sites in the transmembrane (TM) domain and the C-terminal amphipathic helix (AH) of wild-type (WT) and mutant M2, and collected information on line shapes, relaxation rates, membrane topology, and distances within the homotetramer in membranes with and without cholesterol. Our results identify marked differences in the conformation and dynamics between the WT and the alanine mutant. Compared to WT, the dominant population of the mutant AH is more dynamic, shallower in the membrane, and has altered quaternary arrangement of the C-terminal domain. While the AH becomes more dynamic, the dominant population of the TM domain of the mutant is immobilized. The presence of cholesterol changes the conformation and dynamics of the WT protein, while the alanine mutant is insensitive to cholesterol. These findings provide new insight into how M2 may facilitate budding. We propose the AH–membrane interaction modulates the arrangement of the TM helices, effectively stabilizing a conformational state that enables M2 to facilitate viral budding. Antagonizing the properties of the AH that enable interdomain coupling within M2 may therefore present a novel strategy for anti-influenza drug design

    Magnetic field dependence of the internal quality factor and noise performance of lumped-element kinetic inductance detectors

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    We present a technique for increasing the internal quality factor of kinetic inductance detectors (KIDs) by nulling ambient magnetic fields with a properly applied magnetic field. The KIDs used in this study are made from thin-film aluminum, they are mounted inside a light-tight package made from bulk aluminum, and they are operated near 150 mK150 \, \mathrm{mK}. Since the thin-film aluminum has a slightly elevated critical temperature (Tc=1.4 KT_\mathrm{c} = 1.4 \, \mathrm{K}), it therefore transitions before the package (Tc=1.2 KT_\mathrm{c} = 1.2 \, \mathrm{K}), which also serves as a magnetic shield. On cooldown, ambient magnetic fields as small as approximately 30 μT30 \, \mathrm{\mu T} can produce vortices in the thin-film aluminum as it transitions because the bulk aluminum package has not yet transitioned and therefore is not yet shielding. These vortices become trapped inside the aluminum package below 1.2 K1.2 \, \mathrm{K} and ultimately produce low internal quality factors in the thin-film superconducting resonators. We show that by controlling the strength of the magnetic field present when the thin film transitions, we can control the internal quality factor of the resonators. We also compare the noise performance with and without vortices present, and find no evidence for excess noise beyond the increase in amplifier noise, which is expected with increasing loss.Comment: 5 pages, 4 figure

    A Titanium Nitride Absorber for Controlling Optical Crosstalk in Horn-Coupled Aluminum LEKID Arrays for Millimeter Wavelengths

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    We discuss the design and measured performance of a titanium nitride (TiN) mesh absorber we are developing for controlling optical crosstalk in horn-coupled lumped-element kinetic inductance detector arrays for millimeter-wavelengths. This absorber was added to the fused silica anti-reflection coating attached to previously-characterized, 20-element prototype arrays of LEKIDs fabricated from thin-film aluminum on silicon substrates. To test the TiN crosstalk absorber, we compared the measured response and noise properties of LEKID arrays with and without the TiN mesh. For this test, the LEKIDs were illuminated with an adjustable, incoherent electronic millimeter-wave source. Our measurements show that the optical crosstalk in the LEKID array with the TiN absorber is reduced by 66\% on average, so the approach is effective and a viable candidate for future kilo-pixel arrays.Comment: 7 pages, 5 figures, accepted for publication in the Journal of Low Temperature Physic

    Horn-Coupled, Commercially-Fabricated Aluminum Lumped-Element Kinetic Inductance Detectors for Millimeter Wavelengths

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    We discuss the design, fabrication, and testing of prototype horn-coupled, lumped-element kinetic inductance detectors (LEKIDs) designed for cosmic microwave background (CMB) studies. The LEKIDs are made from a thin aluminum film deposited on a silicon wafer and patterned using standard photolithographic techniques at STAR Cryoelectronics, a commercial device foundry. We fabricated twenty-element arrays, optimized for a spectral band centered on 150 GHz, to test the sensitivity and yield of the devices as well as the multiplexing scheme. We characterized the detectors in two configurations. First, the detectors were tested in a dark environment with the horn apertures covered, and second, the horn apertures were pointed towards a beam-filling cryogenic blackbody load. These tests show that the multiplexing scheme is robust and scalable, the yield across multiple LEKID arrays is 91%, and the noise-equivalent temperatures (NET) for a 4 K optical load are in the range 26\thinspace\pm6 \thinspace \mu \mbox{K} \sqrt{\mbox{s}}

    A millimeter-wave kinetic inductance detector camera for long-range imaging through optical obscurants

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    Millimeter-wave imaging provides a promising option for long-range target detection through optical obscurants such as fog, which often occur in marine environments. Given this motivation, we are currently developing a 150 GHz polarization-sensitive imager using a relatively new type of superconducting pair-breaking detector, the kinetic inductance detector (KID). This imager will be paired with a 1.5 m telescope to obtain an angular resolution of 0.09° over a 3.5° field of view using 3,840 KIDs. We have fully characterized a prototype KID array, which shows excellent performance with noise strongly limited by the irreducible fluctuations from the ambient temperature background. Full-scale KID arrays are now being fabricated and characterized for a planned demonstration in a maritime environment later this year

    A LEKID-based CMB instrument design for large-scale observations in Greenland

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    We present the results of a feasibility study, which examined deployment of a ground-based millimeter-wave polarimeter, tailored for observing the cosmic microwave background (CMB), to Isi Station in Greenland. The instrument for this study is based on lumped-element kinetic inductance detectors (LEKIDs) and an F/2.4 catoptric, crossed-Dragone telescope with a 500 mm aperture. The telescope is mounted inside the receiver and cooled to < 4<\,4 K by a closed-cycle 4^4He refrigerator to reduce background loading on the detectors. Linearly polarized signals from the sky are modulated with a metal-mesh half-wave plate that is rotated at the aperture stop of the telescope with a hollow-shaft motor based on a superconducting magnetic bearing. The modular detector array design includes at least 2300 LEKIDs, and it can be configured for spectral bands centered on 150~GHz or greater. Our study considered configurations for observing in spectral bands centered on 150, 210 and 267~GHz. The entire polarimeter is mounted on a commercial precision rotary air bearing, which allows fast azimuth scan speeds with negligible vibration and mechanical wear over time. A slip ring provides power to the instrument, enabling circular scans (360 degrees of continuous rotation). This mount, when combined with sky rotation and the latitude of the observation site, produces a hypotrochoid scan pattern, which yields excellent cross-linking and enables 34\% of the sky to be observed using a range of constant elevation scans. This scan pattern and sky coverage combined with the beam size (15~arcmin at 150~GHz) makes the instrument sensitive to 5<ℓ<10005 < \ell < 1000 in the angular power spectra

    The Detector System for the Stratospheric Kinetic Inductance Polarimeter (SKIP)

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    The Stratospheric Kinetic Inductance Polarimeter (SKIP) is a proposed balloon-borne experiment designed to study the cosmic microwave background, the cosmic infrared background and Galactic dust emission by observing 1133 square degrees of sky in the Northern Hemisphere with launches from Kiruna, Sweden. The instrument contains 2317 single-polarization, horn-coupled, aluminum lumped-element kinetic inductance detectors (LEKID). The LEKIDs will be maintained at 100 mK with an adiabatic demagnetization refrigerator. The polarimeter operates in two configurations, one sensitive to a spectral band centered on 150 GHz and the other sensitive to 260 and 350 GHz bands. The detector readout system is based on the ROACH-1 board, and the detectors will be biased below 300 MHz. The detector array is fed by an F/2.4 crossed-Dragone telescope with a 500 mm aperture yielding a 15 arcmin FWHM beam at 150 GHz. To minimize detector loading and maximize sensitivity, the entire optical system will be cooled to 1 K. Linearly polarized sky signals will be modulated with a metal-mesh half-wave plate that is mounted at the telescope aperture and rotated by a superconducting magnetic bearing. The observation program consists of at least two, five-day flights beginning with the 150 GHz observations.Comment: J Low Temp Phys DOI 10.1007/s10909-013-1014-3 The final publication is available at link.springer.co
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