402 research outputs found
Importance Of Recruitment To Accurately Predict The Impacts Of Human-Caused Mortality On Wolf Populations
Reliable analyses can help wildlife managers make good decisions, which are particularly critical for controversial decisions such as wolf (Canis lupus) harvest. Creel and Rotella (2010) recently predicted substantial population declines in Montana wolf populations due to harvest, in contrast to predictions made by Montana Fish, Wildlife and Parks (MFWP). Here we replicate their analyses considering only those years in which field monitoring was consistent, and we consider the effect of annual variation in recruitment on wolf population growth. We also use model selection to evaluate models of recruitment and human-caused mortality rates in wolf populations in the Northern Rocky Mountains. Using data from 27 area-years of intensive wolf monitoring, we show that variation in both recruitment and human-caused mortality affect annual wolf population growth rates and that human-caused mortality rates have increased with the sizes of wolf populations. We also show that either recruitment rates have decreased with population sizes or that the ability of current field resources to document recruitment rates has recently become less successful as the number of wolves in the region has increased. Predictions of wolf population growth in Montana from our top models are consistent with field observations and estimates previously made by MFWP. Familiarity with limitations of raw data helps generate more reliable inferences and conclusions in analyses of publicly-available datasets. Additionally, development of efficient monitoring methods for wolves is a pressing need, so that analyses such as ours will be possible in future years when fewer resources will be available for monitoring
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The contribution of melt ponds to enhanced Arctic sea-ice melt during the Last Interglacial
The Hadley Centre Global Environment Model version 3 (HadGEM3) is the first coupled climate model to simulate an ice-free Arctic during the Last Interglacial (LIG), 127 000 years ago. This simulation appears to yield accurate Arctic surface temperatures during the summer season. Here, we investigate the causes and impacts of this extreme simulated ice loss. We find that the summer ice melt was predominantly driven by thermodynamic processes: atmospheric and ocean circulation changes did not significantly contribute to the ice loss. We demonstrate these thermodynamic processes were significantly impacted by melt ponds, which formed on average 8 d earlier during the LIG than during the pre-industrial control (PI) simulation. This relatively small difference significantly changed the LIG surface energy balance and impacted the albedo feedback. Compared to the PI simulation: in mid-June, of the absorbed flux at the surface over ice-covered cells (sea-ice concentration > 0.15), ponds accounted for 45 %–50 %, open water 35 %–45 %, and bare ice and snow 5 %–10 %. We show that the simulated ice loss led to large Arctic sea surface salinity and temperature changes. The sea surface temperature and salinity signals we identify here provide a means to verify, in marine observations, if and when an ice-free Arctic occurred during the LIG. Strong LIG correlations between spring melt pond and summer ice area indicate that, as Arctic ice continues to thin in future, the spring melt pond area will likely become an increasingly reliable predictor of the September sea-ice area. Finally, we note that models with explicitly modelled melt ponds seem to simulate particularly low LIG sea-ice area. These results show that models with explicit (as opposed to parameterised) melt ponds can simulate very different sea-ice behaviour under forcings other than the present day. This is of concern for future projections of sea-ice loss
The coupled system response to 250 years of freshwater forcing: Last Interglacial CMIP6–PMIP4 HadGEM3 simulations
The lig127k-H11 simulation of the Paleoclimate Modelling Intercomparison Project (PMIP4) is run using the HadGEM3-GC3.1 model. We focus on the coupled system response to the applied meltwater forcing. We show here that the coupling between the atmosphere and the ocean is altered in the hosing experiment compared to a Last Interglacial simulation with no meltwater forcing applied. Two aspects in particular of the atmosphere–ocean coupling are found to be affected: Northern Hemisphere (NH) gyre heat transport and Antarctic sea ice area. We apply 0.2 Sv of meltwater forcing across the North Atlantic during a 250-year-long simulation. We find that the strength of the Atlantic Meridional Overturning Circulation (AMOC) is reduced by 60 % after 150 years of meltwater forcing, with an associated decrease of 0.2 to 0.4 PW in meridional ocean heat transport at all latitudes. The changes in ocean heat transport affect surface temperatures. The largest increase in the meridional surface temperature gradient occurs between 40–50∘ N. This increase is associated with a strengthening of 20 % in 850 hPa winds. The jet stream intensification in the Northern Hemisphere in return alters the temperature structure of the ocean by increasing the gyre circulation at the mid-latitudes and the associated heat transport by +0.1–0.2 PW, and it decreases the gyre circulation at high latitudes with a decrease of ocean heat transport of −0.2 PW. The changes in meridional surface temperature and pressure gradients cause the Intertropical Convergence Zone (ITCZ) to move southward, leading to stronger westerlies and a more positive Southern Annual Mode (SAM) in the Southern Hemisphere (SH). The positive SAM influences sea ice formation, leading to an increase in Antarctic sea ice.</p
Sexual orientation and the integrated motivational-volitional model of suicidal behavior : results from a cross-sectional study of young adults in the United Kingdom
Sexual orientation minority (i.e., lesbian, gay, bisexual, queer and other; LGBQ+) persons represent a vulnerable population with respect to suicide-related behavior. An emerging theory of suicide, the Integrated Motivational-Volitional Model of Suicide (IMV; O’Connor, 2011; O’Connor & Kirtley, 2018), is utilized in the present study to examine sexual orientation, as well as a number of other IMV-defined pre-motivational factors (i.e., demographics, psychological distress and personality), as they impact the IMV motivational factors of defeat, entrapment, and suicidal ideation/intent. The present investigation featured a cross-sectional online survey of young adults (ages 18 to 34; n = 418; 27% identified as LGBTQ+) across the United Kingdom. The key findings included: (1) high rates of 12-month suicidal ideation prevalence (54.5%) and willingness to enact a future suicide attempt (60.8%); (2) bisexual and other (e.g., pansexual)-identifying sexual minority persons reported higher levels of IMV-related outcomes (e.g., internal entrapment, defeat); (3) sexual orientation accounted for significant variance in predicting motivational constructs controlling for a number of other pre-motivational factors; (4) other sexual minority status, compared to heterosexual identity, predicted all motivational outcomes, and; (5) extraversion, agreeableness, and emotional stability emerged as pre-motivational protective factors for varying motivational outcomes. Findings are discussed with respect to the suicide and sexual minority theories, as well as tailored suicide prevention efforts and future research
Ethical and methodological issues in engaging young people living in poverty with participatory research methods
This paper discusses the methodological and ethical issues arising from a project that focused on conducting a qualitative study using participatory techniques with children and young people living in disadvantage. The main aim of the study was to explore the impact of poverty on children and young people's access to public and private services. The paper is based on the author's perspective of the first stage of the fieldwork from the project. It discusses the ethical implications of involving children and young people in the research process, in particular issues relating to access and recruitment, the role of young people's advisory groups, use of visual data and collection of data in young people's homes. The paper also identifies some strategies for addressing the difficulties encountered in relation to each of these aspects and it considers the benefits of adopting participatory methods when conducting research with children and young people
Captive breeding of Margaritifera auricularia (Spengler, 1793) and its conservation importance
Margaritifera auricularia is one of the most endangered freshwater mussels (Bivalvia, Unionida) in the world. Since 2013, the abundance of this species in the Ebro River basin (Spain) has sharply declined, driving the species to the verge of regional extinction. Therefore, any management measures that might facilitate the recovery of this species would be essential for its conservation.
During 2014–2016, captive breeding of M. auricularia allowed the production of >106 juveniles, out of which 95% were released into the natural environment, and 5% were grown in the laboratory under controlled conditions. The aim of this experimental work was to establish the best culture conditions for the survival and growth of M. auricularia juveniles in the laboratory.
The experiment was divided into two phases: phase I, in which juveniles recently detached from fish gills were cultured in detritus boxes until they reached a shell length of 1 mm; and phase II, in which these specimens were transferred to larger aquaria to grow up to 3–4 mm.
The best experimental conditions for juvenile survival and growth corresponded to treatments in glass containers at a density of 0.2 ind. L−1, using river water, with added substrate and detritus, enriched with phytoplankton, and avoiding extra aeration. The highest survival and growth rates attained, respectively, values of c. 60% at 100 days and 2.56 mm in shell length at 30–32 weeks.
This is the first study to report on the long‐term survival and growth of juvenile M. auricularia in the laboratory, providing essential information in order to implement future conservation measures addressed at reinforcing the natural populations of this highly threatened species in European water bodies.This project was funded by the Government of Aragón, Department of Rural Development and Sustainability and carried out by the Environmental Service Department of SARGA. Special thanks go to Manuel Alcántara, Miguel Ángel Muñoz, Ester Ginés, Carlos Catalá, and Juan Pablo de la Roche, who were involved in the project. The authors appreciate the work of the reviewer and editor who improved the quality of the manuscript. The Aragón's forest rangers are thanked for their assistance during fieldwork
Growth factor restriction impedes progression of wound healing following cataract surgery: identification of VEGF as a putative therapeutic target
Secondary visual loss occurs in millions of patients due to a wound-healing response, known as posterior capsule opacification (PCO), following cataract surgery. An intraocular lens (IOL) is implanted into residual lens tissue, known as the capsular bag, following cataract removal. Standard IOLs allow the anterior and posterior capsules to become physically connected. This places pressure on the IOL and improves contact with the underlying posterior capsule. New open bag IOL designs separate the anterior capsule and posterior capsules and further reduce PCO incidence. It is hypothesised that this results from reduced cytokine availability due to greater irrigation of the bag. We therefore explored the role of growth factor restriction on PCO using human lens cell and tissue culture models. We demonstrate that cytokine dilution, by increasing medium volume, significantly reduced cell coverage in both closed and open capsular bag models. This coincided with reduced cell density and myofibroblast formation. A screen of 27 cytokines identified nine candidates whose expression profile correlated with growth. In particular, VEGF was found to regulate cell survival, growth and myofibroblast formation. VEGF provides a therapeutic target to further manage PCO development and will yield best results when used in conjunction with open bag IOL designs
Development of resistance to type II JAK2 inhibitors in MPN depends on AXL kinase and is targetable.
PURPOSE
Myeloproliferative neoplasms (MPN) dysregulate JAK2 signaling. Since clinical JAK2 inhibitors have limited disease-modifying effects, type II JAK2 inhibitors such as CHZ868 stabilizing inactive JAK2 and reducing MPN clones, gain interest. We studied whether MPN cells escape from type ll inhibition.
METHODS
MPN cells were continuously exposed to CHZ868. We used phosphoproteomic analyses and ATAC-/RNA-sequencing to characterize acquired resistance to type II JAK2 inhibition, and targeted candidate mediators in MPN cells and mice.
RESULTS
MPN cells showed increased IC50 and reduced apoptosis upon CHZ868 reflecting acquired resistance to JAK2 inhibition. Among >2500 differential phospho-sites, MAPK pathway activation was most prominent, while JAK2-STAT3/5 remained suppressed. Altered histone occupancy promoting AP-1/GATA binding motif exposure associated with upregulated AXL kinase and enriched RAS target gene profiles. AXL knockdown resensitized MPN cells and combined JAK2/AXL inhibition using bemcentinib or gilteritinib reduced IC50 to levels of sensitive cells. While resistant cells induced tumor growth in NSG mice despite JAK2 inhibition, JAK2/AXL inhibition largely prevented tumor progression. Since inhibitors of MAPK pathway kinases such as MEK are clinically used in other malignancies, we evaluated JAK2/MAPK inhibition with trametinib to interfere with AXL-MAPK-induced resistance. Tumor growth was halted similarly to JAK2/AXL inhibition and in a systemic cell line-derived mouse model, marrow infiltration was decreased supporting dependency on AXL-MAPK.
CONCLUSIONS
We report on a novel mechanism of AXL-MAPK-driven escape from type II JAK2 inhibition, which is targetable at different nodes. This highlights AXL as mediator of acquired resistance warranting inhibition to enhance sustainability of JAK2 inhibition in MPN
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