Introducing Programming In The AIS Course: Creating And Coding An Expert System Using Visual Basic.NET

The primary purpose of this paper is to provide a stand-alone, introductory tutorial exercise that can be used to give AIS students a very basic understanding of Visual Basic.NET.  The tutorial demonstrates Visual Basic.NET in the context of developing a simple expert system to evaluate loans, thus giving the student exposure to expert systems concepts and design as well.  This paper provides a discussion of the potential reasons for including an introduction to programming in the AIS course, gives more detail about the tutorial and provides guidance for its use in the classroom

Formin-based control of the actin cytoskeleton during cytokinesis

Cytokinesis, the terminal event in the canonical cell cycle, physically separates daughter cells following mitosis. For cleavage to occur in many eukaryotes, a cytokinetic ring must assemble and constrict between divided genomes. Although dozens of different molecules localize to and participate within the cytokinetic ring, the core machinery comprises linear actin filaments. Accordingly, formins, which nucleate and elongate F-actin (filamentous actin) for the cytokinetic ring, are required for cytokinesis in diverse species. In the present article, we discuss specific modes of formin-based actin regulation during cell division and highlight emerging mechanisms and questions on this topic. © 2013 Biochemical Society

The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament capping protein gated by profilin

Cytokinesis in most eukaryotes requires the assembly and contraction of a ring of actin filaments and myosin II. The fission yeast Schizosaccharomyces pombe requires the formin Cdc12p and profilin (Cdc3p) early in the assembly of the contractile ring. The proline-rich formin homology (FH) 1 domain binds profilin, and the FH2 domain binds actin. Expression of a construct consisting of the Cdc12 FH1 and FH2 domains complements a conditional mutant of Cdc12 at the restrictive temperature, but arrests cells at the permissive temperature. Cells overexpressing Cdc12(FH1FH2)p stop growing with excessive actin cables but no contractile rings. Like capping protein, purified Cdc12(FH1FH2)p caps the barbed end of actin filaments, preventing subunit addition and dissociation, inhibits end to end annealing of filaments, and nucleates filaments that grow exclusively from their pointed ends. The maximum yield is one filament pointed end per six formin polypeptides. Profilins that bind both actin and poly-l-proline inhibit nucleation by Cdc12(FH1FH2)p, but polymerization of monomeric actin is faster, because the filaments grow from their barbed ends at the same rate as uncapped filaments. On the other hand, Cdc12(FH1FH2)p blocks annealing even in the presence of profilin. Thus, formins are profilin-gated barbed end capping proteins with the ability to initiate actin filaments from actin monomers bound to profilin. These properties explain why contractile ring assembly requires both formin and profilin and why viability depends on the ability of profilin to bind both actin and poly-l-proline

Effects of Grazing Management on Selected Stream Bank Characteristics and Stream Bank Erosion

Six 30-acre cool-season grass pastures, containing predominantly smooth bromegrass and bisected by a 642- foot stream segment, were grouped into 2 blocks and assigned one of three treatments: continuous stocking - unrestricted stream access (CSU), continuous stocking - restricted stream access (CSR), and rotational stocking (RS). Stream bank condition and surface roughness and stream morphology were evaluated pre-, mid-, and post-grazing from 2005 to 2007. Stream bank erosion was monitored monthly from May through November over the same three years. Stream banks in CSU pastures had greater vegetative cover, stability, and condition scores than did the CSR or RS pastures implying that the stream banks in pastures in which cattle had unrestricted access were more susceptible to erosion than stream banks in pastures in which cattle access to stream banks was restricted or controlled. However, no effect of grazing management on the rate of change of stream cross sectional area, net stream bank erosion, or erosion deposition activity was observed in any of the three years

Effects of Grazing Management on Selected Stream Bank Characteristics and Stream Bank Erosion

Six 30-acre cool-season grass pastures, containing predominantly smooth bromegrass and bisected by a 642- foot stream segment were grouped into two blocks and assigned one of three treatments: continuous stocking - unrestricted stream access (CSU), continuous stocking - restricted stream access (CSR), and rotational stocking (RS). Stream bank condition and surface roughness and stream morphology were evaluated pre-, mid-, and postgrazing over a two-year period. Stream bank erosion was monitored monthly from May through November over the same two-year period. Stream banks in CSU pastures had greater vegetative cover, stability, and condition scores than did the CSR or RS pastures, implying that the stream banks in pastures in which cattle had unlimited access were more susceptible to erosion than stream banks in pastures in which cattle access to stream banks was restricted or controlled. However, no effect of grazing management on net stream bank erosion was observed for either grazing season

C/EBPβ-1 promotes transformation and chemoresistance in Ewing sarcoma cells.

CEBPB copy number gain in Ewing sarcoma was previously shown to be associated with worse clinical outcome compared to tumors with normal CEBPB copy number, although the mechanism was not characterized. We employed gene knockdown and rescue assays to explore the consequences of altered CEBPB gene expression in Ewing sarcoma cell lines. Knockdown of EWS-FLI1 expression led to a decrease in expression of all three C/EBPβ isoforms while re-expression of EWS-FLI1 rescued C/EBPβ expression. Overexpression of C/EBPβ-1, the largest of the three C/EBPβ isoforms, led to a significant increase in colony formation when cells were grown in soft agar compared to empty vector transduced cells. In addition, depletion of C/EBPβ decreased colony formation, and re-expression of either C/EBPβ-1 or C/EBPβ-2 rescued the phenotype. We identified the cancer stem cell marker ALDH1A1 as a target of C/EBPβ in Ewing sarcoma. Furthermore, increased expression of C/EBPβ led to resistance to chemotherapeutic agents. In summary, we have identified CEBPB as an oncogene in Ewing sarcoma. Overexpression of C/EBPβ-1 increases transformation, upregulates expression of the cancer stem cell marker ALDH1A1, and leads to chemoresistance

Phosphorus source--sink relationships of stream sediments in the Rathbun Lake watershed in southern Iowa, USA

The surface waters of Rathbun Lake watershed in southern Iowa are impacted by agricultural sources of sediments and nutrients, including phosphorus (P). Because stream sediments often play an important role in regulating P concentrations in stream water, we investigated sediment-water column P relationships in four creeks within the watershed and then evaluated the relationship between sediment properties and indicators of the risk of P loss. Based on Mehlich-3-extractable P (17 to 68 mg kg -1) and degree of P saturation (2 to 12 %), stream bank and bed sediments at the four sites were unlikely to serve as major sources of P. However, equilibrium P concentrations, which ranged from 0.02 to 0.12 mg L-1, indicated that bed sediments could release P to the water column depending on dissolved P (DP) concentrations in the stream water and the time of year. The likelihood of P desorption from the sediments increased with increasing pH (r=0.92, p \u3c 0.01) and sand content (r = 0.78, p \u3c 0.05), but decreased with clay content (r = −0.72, p \u3c 0.05) and iron (Fe) (r = −0.93, p \u3c 0.001) associated with organic matter. From these results, we speculate that changes in land use within the riparian areas may, at least initially, have little effect on P concentrations in the streams. Low concentrations of DP relative to total P (TP) in these streams, however, suggest that P loads to Rathbun Lake can be reduced if P inputs from eroded bank sediments are controlled

Psychosocial Contexts of Diabetes and Older Adulthood: Reciprocal Effects

The present study was conducted to assess the reciprocal effects between the psychosocial contexts of diabetes and older adulthood. Data were collected from 191 community-dwelling adults over the age of 60 with non-insulin-dependent diabetes mellitus. Results indicate that older adults with diabetes reported higher rates of selected chronic illnesses, lower self-rated physical health, and higher levels of depression than did comparison samples of older adults without diabetes. Compared with younger adults with NIDDM, the present sample of older adults perceived fewer impacts of diabetes, including fewer symptoms of poor metabolic control, less emotional impact, fewer barriers to adherence, and less complex regimens. Overall levels of social support and regimen adherence were high. Older adults in this sample reported wanting minimal help from their family and friends with self-management activities and receiving more help than desired with following a meal plan and taking medications. Implications of the unique context of older adulthood for diabetes self-management are discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/69058/2/10.1177_014572179101700507.pd